EMAGE mouse gene expression spatial database

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EMAGE:7118

Ppef2 protein phosphatase, EF hand calcium-binding domain 2 ( MGI:1342304)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002609. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002609_01	euxassay_002609_02	euxassay_002609_03	euxassay_002609_04

euxassay_002609_05	euxassay_002609_06	euxassay_002609_07	euxassay_002609_08	euxassay_002609_09

euxassay_002609_10	euxassay_002609_11	euxassay_002609_12	euxassay_002609_13	euxassay_002609_14

euxassay_002609_15	euxassay_002609_16	euxassay_002609_17	euxassay_002609_18	euxassay_002609_19

euxassay_002609_20	euxassay_002609_21	euxassay_002609_22	euxassay_002609_23	euxassay_002609_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7118_wholemount_strong.wlz

7118_wholemount_moderate.wlz

7118_wholemount_weak.wlz

7118_wholemount_possible.wlz

7118_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7118_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5233
Entity Detected:	Ppef2, protein phosphatase, EF hand calcium-binding domain 2 ( MGI:1342304)
Sequence:	sense strand is shown >T5233 CCAGGGTGTTGCTGCCCACGGCTTGAAGGTGGACATTGTGATTGTCACATGGCACTTGAAATGCATTGCC AGGGTAGTCGGTGTCATCCTGCTCCCTGAGAAGACAACATCAGACAAAGGTTAGACCTGACCCCATCAGG CAGTTCCATCCACCAACCGTGAAGAAAGATGGTGATCAGACATGGGTTAGGTGCCTGCCTCCTCGTGTCT CAGAGTCAGCTAAGCTCCTGGAAGCAAACCCCTCCGTTCCCTCGGATCGGGATCCGCCACTGCCGTCCAT GTCCTGCAGGAGCGCTGTCCCTCAGCCATGGGAAGCAGCTCATCCACCCAGCACCACTTTGCGTTTCAGA ACGCTGAGAAAGCCTTCAAGGCAGCGGCCCTGATCCAGAGATGGTACCGGCGCTACATGGCCCGCCTAGA GATGAGGAGGCGATGTACCTGGAACATCTTCCAGTCTATAGAGTATGCTGGACAGCAAGACCAGGTCAAG CTCCATGAATTCTTCAGCTACCTTGTGGACCATTTCACTCCCAGCAGCCACCATGAGAGAGATTTCCTGA ACCGCATGTTCACCGAAGAGAGATTCGCCCAGGACGTGGAGACAGAGGAGGGTGGAGATTTTGAATCCAT AGAGGTGCCAGACAGCTACACGGGGCCACGCCTCTCCTTCCCCCTTCTTCCTGACCATGCCACTGCCCTG GTGGAAGCTTTCAGGCTGCGACAACAGCTCCATGCTCGATACGTCTTGAATCTTCTGTACGAGACCAGAA AACACCTGGCCCAGCTGCCCAACATCAACCGAGTCTCCACCTGCTACAGCGAGGAGGTCACAGTGTGTGG AGATCTACATGGCCAGTTGGATGATTTAATATTTATATTTTATAAGAACGGTCTACCATCACCAGAGAGA GCATACGTGTTCAATGGTGACTTTGTGGATCGAGGCAAGGACTCTGTAGAGGTCCTGATGGTTCTCTTTG CCTTCATGTTGGTATACCCCAAGGAGTTCCATCTTAACAGAGGAAACCATGAGGACCATCTAGTGAACCT ACGATATGGCTTCACCAAGGAAGTGATGCATAAATACAAGATACATGGGAAGAAAATCCTAAGGACACTT CAGGATGTCTTCTGCTGGCTTCCGTTGGCCACTCTGGTTGATGAGAAAGTCCTTGTTCTTCATGGTGGAG TCTCAGACAAGACAGACCTGGAACTTCTGGCTAAACTAGACAGGCACAAGATTGTTTCTACCATGAGGTG CAAAACAAGAAAGGAAAGTGAGAATCGAGAGGAGCAGAAGAGAAAAGATAACCAGACAAGCTCTGGACAG AAACCTACTCCGTGGTTTCTTCCTCAAAGCCGCTCTCTGCCCTCTTCGCCTTTTCACCTGGGCTCTGGCT TTAAGGCCTACAAAGCCGGCAGGTCCTGCAGCATCCCCTGTGGCTCCCCTAACAGCAAGGAGCTATCCCG GCGGGGGCAGGTGCGGCGCTCAGTGGACCTGGAACTGGAACAGTGCCGGCAGCAAGCCGGCTTCCTGGGG ATCAGAGAGAAGGGGGAGTCCTTGCCTTTGGCCCCAGATGCTGACTGTGTTGCTGATGGGGGAGGGGTGC TGGAACCCACCCCCGAGGAGTGGAAGCAGGTTGTAGATATTCTGTGGAGTGATCCCGCGGCTCAGGAGGG CTGCAAGGCCAACGCTGTCCGAGGAGGAGGCTGTTACTTTGGGCCTGACGTGACAGAACGGTTGATGGAA AAATACAAGCTGCAACTCCTGATCCGTTCGCATGAGTGCAAACCCGAGGGCTATGAGTTCTGTCACAACC GCAAGGTGTTAACCATCTTTTCTGCCTCCAACTACTATGAAGTTGGCAGCAACAGAGGAGCCTACGTCAA GCTGGGGCCAGCCCTGACTCCACATATCGTGCAGTATCAAGCTAACAAGGCGACCCACAGGCTAACCATG AGGCAAAGGATCAGCAGGGTGGAGGAGTCGGCCCTCAGAGCCCTTCGACAGAAATTATTTGCTCATTCGT CGGACCTGCTTGTTGAGTTTAGGAAGCGCGACCCGGATGAGAGCGGTGTCATCACCCTGAGTGATTGGGC GACTGCCGTGGAGTCTGTGCTGCACCTGGGACTGCCCTGGCGGATGCTGCGGCCACAACTGGTGAATAGT TCAGCGGATAACGTGTTGGAATACAGGTCCTGGTTGGACAGTTTGGCCAAAGAACAGCTGAGCCGAGAGA ATATTCAGTCGAGTTTGCTGGAAAAGCTCTATCGAAACCGATCCAACTTGGAGACCATTTTTAGGATCAT AGACAGCGATCATTCAGGATTCATCTCCCTGGATGAGTTCAGGCAGACTTGGAAGCTCTTCAGCTCACAT ATGAGCATCGACATCACAGATGACGGCATCTGTGACCTCGCCAGAAGCATCGACTTCAACAAGGATGGCC ACATCGACATCAATGAGTTCCTGGAGGCCTTCCGCCTCGTGGAGCAGTCCTGCTTAGAGGGCCACGCCTC TGCTTGCCTGCAGTCCACAGACACTGCTGAGAGTGGCCATAGCAGTCCAGGCCCATGCTGAGGATAGACT GGTCTATTACTCAAAGTGCCTAAGGCGATGCTGAGTTTATGACTCCACATGGACTTGGACTTTATCCTGA AAATTTGCCACCACAAAGCATTCAAATCAAGAGTTACCTGTTTGGACTGGAGAGATGGTTCAGTGGTTAA GAGCACTGACTGCTCTTCCAGAGGTCCTGAGTTCAATTCCCGGCAACCACATTGTGGTTCATAGTGGATC TGTCAGCCTCTTCTGGTGTGTATGAAGACAGCTACAGTGAAATCATATACATAAAATAAATAATTTAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5356685 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5356685 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7117 same embryo
	EMAGE:7120 same embryo
	EMAGE:7116 same embryo
	EMAGE:7115 same embryo
	EMAGE:7119 same embryo
	EurExpress:euxassay_002609 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source