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EMAGE:7121

Psmb2 proteasome (prosome, macropain) subunit, beta type 2 ( MGI:1347045)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7121 EMAGE:7121 EMAGE:7121 EMAGE:7121 EMAGE:7121
"Pseudo-wholemount" of euxassay_004563. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004563_01 euxassay_004563_02 euxassay_004563_03 euxassay_004563_04
EMAGE:7121 EMAGE:7121 EMAGE:7121 EMAGE:7121 EMAGE:7121
euxassay_004563_05 euxassay_004563_06 euxassay_004563_07 euxassay_004563_08 euxassay_004563_09
EMAGE:7121 EMAGE:7121 EMAGE:7121 EMAGE:7121 EMAGE:7121
euxassay_004563_10 euxassay_004563_11 euxassay_004563_12 euxassay_004563_13 euxassay_004563_14
EMAGE:7121 EMAGE:7121 EMAGE:7121 EMAGE:7121 EMAGE:7121
euxassay_004563_15 euxassay_004563_16 euxassay_004563_17 euxassay_004563_18 euxassay_004563_19
EMAGE:7121 EMAGE:7121 EMAGE:7121
euxassay_004563_20 euxassay_004563_21 euxassay_004563_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7121Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7121_wholemount_strong.wlz
7121_wholemount_moderate.wlz
7121_wholemount_weak.wlz
7121_wholemount_possible.wlz
7121_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7121_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
nucleus pulposus
strong strong
regionalstrong expression: see section 11
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5801
Entity Detected:Psmb2, proteasome (prosome, macropain) subunit, beta type 2 ( MGI:1347045)
Sequence:sense strand is shown

>T5801
CCTGTCCGTGGCCTCTGCAGCCTCCCGCTTCCCGGCACCATGGAGTACCTCATTGGCATCCAGGGCCCAG
ACTATGTCCTCGTCGCTTCCGACCGAGTGGCCGCCAGCAATATTGTCCAGATGAAGGACGATCATGACAA
GATGTTTAAGATGAGTGAAAAAATCTTACTCCTATGTGTTGGAGAGGCTGGAGACACTGTACAGTTTGCA
GAATATATCCAGAAAAACGTGCAACTTTATAAAATGCGGAATGGATATGAATTGTCTCCCACAGCAGCAG
CTAACTTCACACGGCGGAACCTGGCTGACTGTCTTCGGAGTCGGACCCCATATCATGTGAACCTCCTCCT
GGCTGGCTATGACGAGCATGAAGGGCCAGCCCTTTACTACATGGACTACCTGGCAGCCTTGGCTAAGGCT
CCTTTTGCAGCTCATGGCTATGGTGCCTTTCTGACTCTCAGCATCCTTGACCGATACTACACACCTACCA
TCTCACGTGAGAGGGCAGTGGAGCTTCTTAGGAAGTGTCTGGAGGAGCTCCAGAAGCGCTTCATCTTGAA
TCTGCCCACCTTCAGTGTCCGGGTCATTGACAAAGATGGCATCCACAATCTGGAGAACATTGCCTTCCCT
AAGCGGGACTCCTAACGTCACATCCTTCCCTCCCACTCACCAGGGAACTTTTTTGATGGGCTCATTTTTT
TTTTTTTTCTACTCTTTTGACGTTCACTCTTGATAGATGGTTAATTCAAAATAAAGCTGAATATAGCTAA
AAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:2811010 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2811010 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7126 same embryo
 EMAGE:7125 same embryo
 EMAGE:7123 same embryo
 EMAGE:7124 same embryo
 EMAGE:7122 same embryo
 EurExpress:euxassay_004563 same experiment
 MGI:4827475 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS