EMAGE mouse gene expression spatial database

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EMAGE:7123

Smpdl3b sphingomyelin phosphodiesterase, acid-like 3B ( MGI:1916022)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004537. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004537_01	euxassay_004537_02	euxassay_004537_03	euxassay_004537_04

euxassay_004537_05	euxassay_004537_06	euxassay_004537_07	euxassay_004537_08	euxassay_004537_09

euxassay_004537_10	euxassay_004537_11	euxassay_004537_12	euxassay_004537_13	euxassay_004537_14

euxassay_004537_15	euxassay_004537_16	euxassay_004537_17	euxassay_004537_18	euxassay_004537_19

euxassay_004537_20	euxassay_004537_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7123_wholemount_strong.wlz

7123_wholemount_moderate.wlz

7123_wholemount_weak.wlz

7123_wholemount_possible.wlz

7123_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7123_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5799
Entity Detected:	Smpdl3b, sphingomyelin phosphodiesterase, acid-like 3B ( MGI:1916022)
Sequence:	sense strand is shown >T5799 CTTGGAACACTGGAAGCAGCCAAACGAGTAGTTCTGCCCAGACACGTGGCTGGCCACAAGGTGGGAACCC ACGGCGCAGACGGACTTGGAGGAGCTTCGGCCGCCCGCCGTGGGGGGATCCAGAGGATGACGCTGCTCGG GTGGCTGATATTCCTGGCCCCCTGGGGAGTCGCAGGGGCTCAACTAGGGAGGTTCTGGCACATCTCCGAC CTGCATCTGGACCCCAACTACACCGTATCCAAAGACCCCCTCCAGGTGTGCCCGTCGGCCGGCTCCCAGC CTGTGCTAAATGCTGGCCCCTGGGGGGACTACCTCTGCGATTCTCCTTGGGCCCTTATCAACTCGTCCTT GTATGCCATGAAGGAGATTGAACCAAAGCCTGACTTCATTCTCTGGACAGGGGACGACACACCGCACGTC CCCAATGAGAGTCTAGGAGAGGCAGCTGTGCTGGCAATTGTGGAACGCTTGACCAACCTCATCAAGGAAG TCTTTCCAGACACTAAAGTCTATGCTGCTCTGGGAAATCATGACTTCCACCCTAAGAACCAGTTCCCAGC ACAGAGCAACCGCATCTATAACCAGGTGGCAGAGCTGTGGAGACCCTGGCTTAGTAACGAATCCTACGCT CTCTTCAAAAGAGGTGCCTTCTATTCTGAGAAGTTGCCGGGTCCCAGCAGGGCGGGGCGAGTTGTGGTCC TCAACACCAATCTGTACTACAGCAACAACGAGCAGACAGCTGGCATGGCTGACCCCGGCGAGCAGTTCCG GTGGCTGGGAGATGTCCTGAGCAATGCATCTCGGGATGGGGAGATGGTGTATGTTATTGGCCACGTGCCC CCGGGGTTCTTTGAGAAGACACAGAACAAGGCCTGGTTCCGAGAGAGCTTCAATGAGGAGTATCTGAAGG TGATCCAGAAGCACCATCGGGTCATAGCGGGGCAGTTCTTTGGACACCACCATACCGACAGCTTCCGAAT GTTCTATGACAACACAGGTGCCCCCATAAACGTCATGTTTCTCACACCCGGGGTCACACCGTGGAAGACC ACATTACCTGGAGTGGTCGATGGGGCCAACAATCCAGGGATACGCATTTTCGAGTATGATCGAGCCACAC TCAACTTGAAGGACTTGGTGACTTACTTCTTGAACCTGAGGCAGGCGAATGTACAAGAGACCCCACGGTG GGAGCAGGAGTACCGCCTGACGGAGGCCTACCAGGTGCCGGATGCCAGCGTCAGCTCCATGCACACGGCG CTGACCCGCATTGCCAGTGAGCCTCACATCCTGCAACGTTATTACGTCTATAACTCGGTCAGCTACAACC ATTTGACCTGTGAGGACAGCTGCCGCATCGAGCACGTTTGTGCTATACAACACGTGGCTTTCAACACTTA TGCTACCTGCTTGCATGGTCTTGGTGCCAAGCTGGTGCCTGGTTTCCTGCTCATACTGACTCTGCTGCCA AGCCTGCACGTACTGGAGGTGTTATGACTGTAGGCACTGCCAGCCCACCCACTGACTTGGTTCATCTTTC TCCTGGTAAAATTGGGCAACGTTACCCAATGGAGAGAGATGAACTTTTGCCATCAGTCTCCCCCCGCGGA GAGTAAAGTGGGATGGGACACCTGGATTCACCAGAGAGGGCGCTAAGAAGCACAATTTCTTCCAAGTTTC ATGTTTTATCCACAAAACTTGTTTGCGGTACATGTCTTGGTGCATTATTCGCTTTAATACAAACATATCA AACCACCATGAACTTGATCACCACAGTGAAGTGAGCCGACGGGAAGATGGGCCTTGGAGATTCTGTGCTT TCGATGTAGTCCCTGCTCAAGGTCTGAACTGGGAACGTACGTGGTGTGCAAAACCCCTTAGTACTTTTAA ATAAAAGACTTTTTTGATTTGGTTTTGCAACAGTGAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2810972 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2810972 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7126 same embryo
	EMAGE:7125 same embryo
	EMAGE:7124 same embryo
	EMAGE:7122 same embryo
	EMAGE:7121 same embryo
	EurExpress:euxassay_004537 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source