EMAGE mouse gene expression spatial database

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EMAGE:7136

Bnip3l BCL2/adenovirus E1B interacting protein 3-like ( MGI:1332659)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002684. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002684_01	euxassay_002684_02	euxassay_002684_03	euxassay_002684_04

euxassay_002684_05	euxassay_002684_06	euxassay_002684_07	euxassay_002684_08	euxassay_002684_09

euxassay_002684_10	euxassay_002684_11	euxassay_002684_12	euxassay_002684_13	euxassay_002684_14

euxassay_002684_15	euxassay_002684_16	euxassay_002684_17	euxassay_002684_18	euxassay_002684_19

euxassay_002684_20	euxassay_002684_21	euxassay_002684_22	euxassay_002684_23	euxassay_002684_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7136_wholemount_strong.wlz

7136_wholemount_moderate.wlz

7136_wholemount_weak.wlz

7136_wholemount_possible.wlz

7136_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7136_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 11 14 weak expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5298
Entity Detected:	Bnip3l, BCL2/adenovirus E1B interacting protein 3-like ( MGI:1332659)
Sequence:	sense strand is shown >T5298 GATACTGTCGTCCTGCGGCCGTGCCGCTTCCAGTCTCCAGACCATGTCTCACTTAGTCGAGCCGCCGCCG CCCTTGCACAACAACAACAACAACTGCGAGGAAGGGGAGCAGCCCCTGCCGCCGCCCGCTGGCCTCAACA GTTCCTGGGTGGAGCTACCCATGAACAGCAGCAATGGCAATGAGAATGGAAATGGGAAGAATGGGGGGCT GGAGCACGTTCCTTCCTCGTCTTCCATCCACAATGGAGACATGGAGAAGATCCTCCTGGATGCACAGCAT GAGTCGGGACAGAGCAGCTCAAGAGGCAGTTCGCACTGTGACAGCCCTTCACCACAAGAAGATGGGCAGA TCATGTTTGATGTTGAGATGCATACCAGCAGGGACCACAGCTCTCAGTCAGAAGAAGAAGTTGTAGAAGG AGAAAAGGAAGTTGAGGCTTTGAAGAAAAGTGCAGACTGGGTATCAGACTGGTCCAGTAGACCCGAAAAC ATCCCACCCAAAGAGTTCCATTTCAGACACCCTAAACGTGCTGCGTCTCTAAGCATGAGGAAGAGTGGAG CCATGAAGAAAGGGGGCATTTTCTCTGCAGAGTTCCTAAAAGTTTTCATCCCATCTCTCTTCCTTTCTCA TGTGCTGGCCTTGGGGCTCGGCATCTATATTGGAAAACGACTGAGCACACCTTCTGCCAGCACCTACTGA GGGAGACGAAAAGCCCCTGGAAATTCGTGTGGCCTGTGAAGTGGTGTATTGTAGTTTGAATTTGAGACCT ATTGTAAGCATGATCCCTACCTACAACCCTATTTTTACATACCCAATTCCAGTAACTCTCAAATCCAGTA TTTTACTCAAACTCTGTTGGGGCATTTTACTAACCTTGTTCCCTTTTTGGCCTGTAAGACACTTTAGAGA TTCCTAACAGAGTTTACTGTTGTTTAGAAATTTGCAAGGGCTTTTTTTCAGCAATGCCACCAGCAGATTA TAATCTTGTCAACAGTTCATTGTCTCCTCTTAGTACCACCAGTCACCTGCATCATTCATGCTAGAAGCTG TGTTCTAGCCATGCAGTTACATCTCTTTCTAAATGAGGTCAGGTTTGCAAATAATAACAACAAAAAAGCT TTGTTTAGTCTTTGTTTTGTTTTCCAGGACAAAAACAAGCTTCCCTAAGCTTGAATTTATAGTTATAAAA TGAAAACAAAACCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5362586 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5362586 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7133 same embryo
	EMAGE:7137 same embryo
	EMAGE:7138 same embryo
	EMAGE:7135 same embryo
	EMAGE:7134 same embryo
	EurExpress:euxassay_002684 same experiment
	MGI:4823490 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source