EMAGE mouse gene expression spatial database

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EMAGE:7211

Kars lysyl-tRNA synthetase ( MGI:1934754)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002719. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002719_01	euxassay_002719_02	euxassay_002719_03	euxassay_002719_04

euxassay_002719_05	euxassay_002719_06	euxassay_002719_07	euxassay_002719_08	euxassay_002719_09

euxassay_002719_10	euxassay_002719_11	euxassay_002719_12	euxassay_002719_13	euxassay_002719_14

euxassay_002719_15	euxassay_002719_16	euxassay_002719_17	euxassay_002719_18	euxassay_002719_19

euxassay_002719_20	euxassay_002719_21	euxassay_002719_22	euxassay_002719_23	euxassay_002719_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7211_wholemount_strong.wlz

7211_wholemount_moderate.wlz

7211_wholemount_weak.wlz

7211_wholemount_possible.wlz

7211_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7211_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 06 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5899
Entity Detected:	Kars, lysyl-tRNA synthetase ( MGI:1934754)
Sequence:	sense strand is shown >T5899 TCTAGTTCTACGCTAGTCATAATTTTTTTTTTTCTGGGTCGGGCCAGTGGGGAAGATGGCCACGCTGCAG GAGAGTGAAGTCAAAGTGGATGGCGAGCAGAAACTAAGCAAGAATGAGCTGAAGAGGCGTCTGAAAGCTG AGAAGAAACTGGCAGAGAAGGAGGCCAAGCAGAAAGAGCTGAGTGAGAAACAGCTAAACCAGACTGCTTC CGCTCCCAACCACACGGCTGACAATGGCGTGGGTGCTGAGGAGGAGACTCTGGACCCAAATCAATACTAC AAGATCCGAAGTCAAGCCGTCCAGCAGCTGAAGGTCACTGGGGAGGATCCGTACCCACACAAGTTCCACG TGGACATCTCACTCACTCAGTTCATCCAAGAATATAGTCACCTGCAGCCTGGGGACCACCTGACTGATGT CACCCTCAAAGTGGCAGGCCGCATCCACGCCAAGAGGGCCTCTGGAGGGAAGCTCATCTTCTATGACCTG CGAGGAGAGGGGGTCAAGTTACAAGTCATGGCCAACTCCAGGAATTACAAATCAGAGGAGGAATTTGTTC ATATCAATAACAAACTGCGCCGGGGAGACATAATTGGAGTTGAGGGCAATCCCGGGAAAACCAAGAAGGG CGAGCTGAGCATCATCCCCCAGGAGATCACGCTGCTGTCCCCCTGCTTGCACATGCTGCCTCATCTTCAC TTTGGCCTCAAAGACAAGGAAACACGGTATCGTCAAAGATACTTGGACTTGATCCTGAACGACTTTGTGA GGCAGAAATTTATCGTCCGCTCTAAGATCATCACATACATAAGAAGTTTCTTGGATGAGCTGGGCTTCCT AGAGATCGAAACTCCCATGATGAACATCATTCCCGGGGGAGCTGTGGCCAAGCCTTTCATCACCTATCAC AATGAGCTGGACATGAACTTGTATATGAGAATTGCTCCAGAACTCTACCACAAGATGCTGGTGGTTGGTG GCATTGACCGGGTTTATGAAATTGGGCGCCAGTTCCGGAACGAAGGGATTGATTTGACTCACAATCCTGA GTTCACCACCTGTGAGTTCTACATGGCCTATGCAGACTATCATGACCTCATGGAGATCACAGAGAAGATG CTGTCAGGGATGGTGAAGAGCATTACAGGCAGTTACAAGATCACCTACCACCCAGATGGGCCGGAAGGCC AAGCCTACGAGGTCGACTTCACCCCACCCTTCCGAAGAATCAGCATGGTAGAAGAGCTTGAGAAAGCCCT AGGTGTGAAGCTGCCAGAAACCAGTCTCTTTGAAACTGAAGAAACTCGGAAAATTCTTGATGATATTTGT GTTGCAAAAGCTGTTGAATGCCCCCCACCTCGGACCACAGCCAGGCTCCTTGATAAGCTCGTTGGCGAGT TCCTCGAAGTCACATGCATCAGCCCTACCTTCATCTGTGATCACCCACAGATCATGAGTCCTTTGGCCAA ATGGCACCGCTCCAAAGAGGGTCTCACGGAGCGCTTTGAGCTGTTTGTCATGAAGAAGGAGATATGCAAT GCCTATACTGAGCTGAATGACCCCGTGCGGCAGAGGCAGCTGTTTGAGGAGCAGGCCAAGGCCAAGGCTG CCGGTGATGACGAGGCCATGTTCATAGACGAGAACTTCTGTACTGCCCTGGAATACGGGCTGCCTCCCAC AGCGGGCTGGGGCATGGGCATTGATCGGCTCACCATGTTTCTCACCGATTCCAACAATATCAAGGAAGTA CTTCTGTTTCCTGCCATGAAGCCAGAGGACAAGAAGGAAACTGCAGCGACCACTGAAACCCCAGAGAGCA CAGAGGCCAGCCCCTCTGTCTAGAAGGCACATGCAACTATGGTGTCTGCATCTCAGCAGATCTGGTCTGA GGGAGCGATTCCTGTGTGGCGCCGTCCATCTGGCCACTGCAGTCCAACCTCCACAAGAGAGAAGAACTAA AGAAGTTCTTTTTATTCCTCGTTACCAAATAAATCATTTCTCTCTTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3157706 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3157706 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7213 same embryo
	EMAGE:7209 same embryo
	EMAGE:7212 same embryo
	EMAGE:7208 same embryo
	EMAGE:7210 same embryo
	EurExpress:euxassay_002719 same experiment
	MGI:4825689 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source