EMAGE mouse gene expression spatial database

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EMAGE:7212

Gpr137b G protein-coupled receptor 137B ( MGI:1891463)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002728. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002728_01	euxassay_002728_02	euxassay_002728_03	euxassay_002728_04

euxassay_002728_05	euxassay_002728_06	euxassay_002728_07	euxassay_002728_08	euxassay_002728_09

euxassay_002728_10	euxassay_002728_11	euxassay_002728_12	euxassay_002728_13	euxassay_002728_14

euxassay_002728_15	euxassay_002728_16	euxassay_002728_17	euxassay_002728_18	euxassay_002728_19

euxassay_002728_20	euxassay_002728_21	euxassay_002728_22	euxassay_002728_23	euxassay_002728_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7212_wholemount_strong.wlz

7212_wholemount_moderate.wlz

7212_wholemount_weak.wlz

7212_wholemount_possible.wlz

7212_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7212_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lens

moderate

regional

moderate expression: see section 01 02

mandible

moderate

single cell

moderate expression: see section 05 06 07 08 09 10 11 12 13 18 19 20 21 22 23

maxilla

moderate

single cell

moderate expression: see section 07 08 09 10 11 13 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5897
Entity Detected:	Gpr137b, G protein-coupled receptor 137B ( MGI:1891463)
Sequence:	sense strand is shown >T5897 GCGGCGGGAGGAAGTGCGGCTTGTTTTCCCGGCTAGGCTCTGGAGCGGCGGGCGCGGCGCGATGCGCGGG TACCCGGGAGCGAACGGCTGCGAGCCCTGATGAAGCTCGAGCAGCCCCAGCCTGATGGAGGCGCCTCCGT GGGAGCCGGTGCGCAATGACTCCCTGCCTCCCACGCTGAGCCCCGCGGTGCCGCCCTACGTGAAGCTCGG CCTCACCGCGGTCTACACCGTCTTCTACGCGCTCCTCTTCGTGTTCATCTATGCGCAGCTCTGGCTGGTG CTGCGCTACCGTCACAAGCGGCTCAGCTACCAGAGCGTCTTCCTCTTCCTTTGCCTCTTCTGGGCCTCGC TGCGTACCGTGCTCTTCTCCTTTTACTTCCGAGACTTCGTGGCAGCCAACTCGTTCAGCCCCTTCGTCTT CTGGCTGCTCTACTGCTTCCCCGTGTGTCTACAGTTCTTCACCCTCACGCTCATGAACTTGTACTTCACG CAGGTGATTTTCAAGGCCAAGTCAAAATATTCTCCAGAGCTACTCAAATACCGGTTACCCCTCTACCTGG CCTCACTTTTCATCAGCCTCGTTTTCCTGTTGGTGAATCTGACCTGTGCTGTGCTGGTGAAGACGGGAGA CTGGGACAGGAAGGTTATCGTCTCTGTGAGAGTGGCCATCAATGACACACTCTTTGTGCTGTGTGCTATC TCTCTCTCCATCTGCCTCTACAAAATCTCCAAGATGTCCCTGGCCAACATCTACTTGGAGTCAAAGGGCT CATCAGTGTGTCAGGTAACTGCCATTGGTGTCACCGTCATCTTGCTCTACACCTCTCGGGCCTGCTACAA CCTGTTCATCTTGTCATTTTCTCAGATCAAGAACGTCCATTCCTTTGATTATGACTGGTACAATGTATCC GACCAGGCAGATCTGAAGAGCCAGCTGGGTGACGCCGGCTACGTAGTGTTTGGCGTGGTGCTTTTCGTGT GGGAGCTCCTACCCACCACCTTGGTGGTTTATTTCTTCCGAGTCAGAAATCCCACGAAGGATCTTACCAA TCCTGGGATGGTCCCCAGCCATGGATTCAGTCCCAGATCTTACTTCTTTGACAACCCCCGAAGATATGAC AGTGATGATGACCTTGCCTGGAACATTGCCCCTCAGGGACTTCAGGGAAGTTTTGCTCCAGACTACTGTG ATTGGGGACAACAAAATAACAGCTTCCTGGCACAAGCAGGAACTTTGCATCAAGACTCCACTTTGGATCC AGACAAAGCAAGCCAAGGGTAGCAGCAGCTGACACAGCCCTATGGAAGAGTTCTCTGTTGAAAGCCTTCA GCCAGACAGACCGGATGACAGCTGAGTTGCTAAGGCAGTTTTCCTTAGGAAACAGAACTCTAGTTTTTGC TATAGCTTTCTCATGGCTCCACAGGGCTAAGCAATAATTTAGAGCAATAAACTCTTTAGTACTAGCAGAG AATCTGGCTATTTCAGTGGGTATAATTTAAACTTATAAAAGAGGTTCTGTACTTTTATAAAGATGTATTT TATATAACTTAAATACTAATGCTAAAGTATACTAGGTTTTTCCTTGATTGTTAATTGCAACGTATGTTGT AGTTTGCACAGACTTTCATGCATAATTCACTTTAAAACGTATAGAATACGTGGTCTAATAGTTTAAAGCT TTGGGGAAAGTTTCCACAAATCTTACCTCTGAAGGTCCCTCTTGTGAGTGCCACGTGGTGGGCTCCTTTC ACCGCCACTCAAGCATTCCAAGTTCAGGAGAAGCAGAGTACCATGGTCTGTACGTAACAGGCTCAACAGC AGCAGCAGCAGCAGCAGCAGCAGCAGCAATTTCTCGTAAACTCTGTCCTAAGCCTGGTCCTTCTTCATCT GAAAGCACTACTACAAGCACTCCAGTAACAAGTGGATACTGTTAAGATGTAGTTGCTGACACTATTAAAC CTCCTCTGCTGTGTGTGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3157256 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3157256 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7211 same embryo
	EMAGE:7213 same embryo
	EMAGE:7209 same embryo
	EMAGE:7208 same embryo
	EMAGE:7210 same embryo
	EurExpress:euxassay_002728 same experiment
	MGI:4825175 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source