EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7244

Acad10 acyl-Coenzyme A dehydrogenase family, member 10 ( MGI:1919235)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004318_01

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7244_wholemount_possible.wlz

7244_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7244_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5132
Entity Detected:	Acad10, acyl-Coenzyme A dehydrogenase family, member 10 ( MGI:1919235)
Sequence:	sense strand is shown >T5132 CCACGCGTCCGGTCTGGACAGGGTCCGAGGAGCGTGCGCAGAGCACGCAAAGACGCCTGGCTCCTATTTT GCGTTTGACCCCTCTCTACGTTTGTCCTCCTTAGTTTTCCCGCAGGGCTCACCGAGAGTCTATGGGTCCG CAGAGACTCTGAGGCTTCCTGGGTCTTCGACCTCAGGGTTGTAACTTTCTGCAGCCTCCATCTCACCATG TTGGTAAGAAGACTTTTCCAGCCTTCCACTCTGCACTGGGCATGGAGGACCACGGCCCTGAACCATCCCC TGGGCAGGCATCAGGGAGGCCTCCGCTGGACACATTCTGGAGGCCGAAGCTACAGAGCAGTCATTTTTGA CACGGGCGGAGTTCTCGTGCCTTCTCCAGGGACAGTGGCTGTGGGCTGGGAGGTGCAGAATCATGTCCCT TCTGGAACTATAGTGAAGGCTTTCATCAGAGGTGGCGACAGTGGGCCCTGGATAAGGTTTATAAAAGGAG AGATAACGACCGAGCACTTCTTGGAAGAATTCGGGAGACTGTGCTCTGAAATCGCAAAGACTTCCGTGCC CGTGAGCTCCTATTTCTCTCTGCTGACCAGTGAGCAGGTGACAAAGCAGTTCCCGGTGATGACTCAGGCC ATCTCTCAGATCAGAGCAAAAGGCCTCCAGACTGCAGTCCTCACCAACAACTTTCATCTTTCCAGCGGGG AAAGCTTCCTGCCCCTGGACCGGAAACAGTTTGATGTGGTGGTTGAGTCCTGCCTGGAAGGCATCTGTAA GCCAGATCCCAGGATATTCCAGCTGTGCCTGCAGAGGCTGAGCCTGCAGCCCTCAGAGGCTATTTTTCTT GATGATCTGGGATCCAATCTAAAAGTGGCTGCCAGCCTGGGCATTCACACCATCAAGGTTGACAGGCCCG AGACAGCAGTGAAGGAGCTGGAGGCTCTACTGGGTTTCCCACTGCACTTGGGCGTTCCCAACACCCGGCC TGTGAGGAAGACCATGGCGATTCCACAAGATGCCTTGGAGAAGTACCTGAAGGGCTTGCTGGGGACCCAC TCCACCGGCCCCATGGAGCTCCTTCAGTTTGATCATGGACAGTCAAACCCAACGTACTACATCAGGCTGG CTGACCGTCAGCTGGTCCTGAGGAAGAAACCCTCGGGGACCCTCCTCCCCTCTGCCCATGCCATAGAAAG GGAATTCAGGATCATGAAAGCCCTTGCAAATGCAGGAGTCCCTGTCCCTACTGTTCTTGACCTCTGTGAA GACTCAAGCATCATCGGCACCCCCTTCTACTTGATGGAGTACTGCCCGGGCATCATCTACAAAGACCCCT CCCTGCCCGGCTTGGAGCCCAGCCGGCGGGAAGCCATATACACTGCCATGAACCAAGTCCTGTGCAGAAT TCACAGTGTGGACCTGCAGGCCACCAGCCTGGACAGCTTCGGCAAGCAAGGGGACTATATTCCTCGACAG GTGCAAACCTGGACCAAGCAGTACCGAGCAGCAGAAACCAGCAGCATCCCAGCAATGGAGAGGCTTATCC AGTGGCTGCCACTGCACCTTCCCAGGCAACAGAGAACCACGCTGGTGCATGGTGACTTCAGGCTGGACAA CCTGATATTCCATCCTGAAAAGGCGGAAGTGCTCGCTGTCCTGGACTGGGAACTTTCCACCTTAGGAGAC CCCTTTGCTGATGTGGCCTACAGCTGCCTGGCCTACTACCTGCCATCCAGCTTTCCCATTCTGAGAGGGT TCAGAGACCAGGATGTGACAAAGCTGGGCATCCCCACTGTGGAGGAATACTTCAGGATGTACTGTCTTAA CATGGGCATCCCACCCATCGACAACTGGAACTTCTACATGGCTTTCTCCTTTTTCCGGGTGGCTGCAATC CTACAGGGAGTCTACAAGCGGTCACTCACAGGCCAGGCAAGCTCAGCAACAGCCCAACAAAGTGGAAAGC TCACTGAGTCCATGGCTGAGCTGGCCTGGGATTTTGCCACCAAAGAAGGGTTCCGGGTTTTCAAAGAGAT GCCAGCCACCAAAACACTGAGCAGATCCTACCATGCCTGGGCGGGACCGAGGTCCCCGAGGACTCCCAAG GGTGTGAGGGGTCACAGCACTGTAGCAGCTGCTTCCCCTTCTCACGAGGCAAAGGGAGGCCTGGTCATCT CTCCAGAGGGCCTGTCTCCTGCGGTCAGAAAGTTGTATGAGCAGCTGGTGCAGTTTATAGAACAGAAGGT GTACCCCCTGGAGCCAGAGCTGCAGAGACACCAGGCCTCGGCCGACAGGTGGAGCCCTTCCCCGCTGATA GAAGACCTCAAGGAGAAAGCCAAGGCTGAGGGACTTTGGAACCTTTTCCTACCCTTGGAGACTGATCCTG AGAAAAAGTACGGAGCAGGGCTGACTAACGTGGAATATGCACATCTGTGTGAAGTGATGGGCATGTCTCT GTATGCTTCTGAGATATTTAATTGCTCTGCCCCGGATACGGGGAATATGGAGATCCTGGTGCGGTATGGC ACTGAGGAGCAGAAGGCCCGCTGGCTGGTGCCACTGTTGGAGGGCAGGATCCGTTCTTGCTTTGCCATGA CTGAGCCTCAGGTGGCCTCCTCAGATGCCTCTAACATCGAGGCTTCCATCAAAGAGGAAGATGGCTGCTA TGTCATAAATGGTCACAAGTGGTGGACCTCAGGCATCCTGGATCCTCGATGCAAACTCTGTGTGTTTATG GGGAAAACAGACCCACAGGCCCCAAGGCACCAGCAACAATCTATGCTCTTGGTTCCCATGGACTCGCCAG GAATCACAGTCATCCGGCCTCTGTCAGTGTTTGGTTTGGAGGATCCTCCAGGTGGCCACGGTGAAGTACG GTTCAAGGACGTGCGTGTGCCCAAGGAAAACATACTCCTGGGCCCTGGCCGAGGCTTTGAGATCGCTCAG GGCAGGCTGGGGCCTGGCCGGATTCATCACTGCATGAGGTTGATTGGGTATTCAGAGAGGGCCCTGGCAC TCATGAAGACACGGGTGATGTCCCGCACGGCCTTCGGGAAGCCCCTTGTGGAGCAGGGCACCATCCTGGC AGACATTGCCCGGTCACGTGTGGAGATCGAGCAGGCCCGGCTGCTGGTGCTGAAAGCTGCTCACCTCATG GATGTGGCCGGAAATAAGACTGCAGCTTTAGACATTGCAATGATTAAAATGGTCGTCCCTTCCATGGCCT ATCACGTAATTGACCGCGCTATTCAGGCCTTTGGAGCAGCGGGCCTCAGCAGTGACTACCCCCTGGCTCA GTTCTTTGGCTGGGCCCGAGCCCTGCGCTTCGCTGATGGCCCTGATGAAGTACACCAGCTGACAGTGGCT AAGATGGAACTGAAGAATCAGTCCAGGATGCAGGAACCAGCAGTGCCCAGAGTGTAGATGCTGAGTTTGG CATGGTCTGCTATGTGTGGTCCTCACATCCTATCCAGCAACTCAGTCTGGGACAGTTACTGTGACCTGAA CCTCCTAGGGTCTTTTCAAGAAGATATTCTCTGGGGTCTCTTCAGAGACCTGCCTGCCTCTGCTTTCCAC GTGCTGGAATTAAATACATTTGCAACTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5343012 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5343012 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7243 same embryo
	EMAGE:7247 same embryo
	EMAGE:7246 same embryo
	EMAGE:7242 same embryo
	EMAGE:7245 same embryo
	EurExpress:euxassay_004318 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source