EMAGE mouse gene expression spatial database

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EMAGE:7246

Prkd3 protein kinase D3 ( MGI:1922542)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004341. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004341_01	euxassay_004341_02	euxassay_004341_03	euxassay_004341_04

euxassay_004341_05	euxassay_004341_06	euxassay_004341_07	euxassay_004341_08	euxassay_004341_09

euxassay_004341_10	euxassay_004341_11	euxassay_004341_12	euxassay_004341_13	euxassay_004341_14

euxassay_004341_15	euxassay_004341_16	euxassay_004341_17	euxassay_004341_18	euxassay_004341_19

euxassay_004341_20	euxassay_004341_21	euxassay_004341_22	euxassay_004341_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7246_wholemount_strong.wlz

7246_wholemount_moderate.wlz

7246_wholemount_weak.wlz

7246_wholemount_possible.wlz

7246_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7246_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5123
Entity Detected:	Prkd3, protein kinase D3 ( MGI:1922542)
Sequence:	sense strand is shown >T5123 CCACGCGTCCGTCTTGATACAGATCAACTATTCTATTTGTTTTTCTGAAATGAAAAGCAAAAGACAAGAA CAGATTTAGAAGAAGTGAAGATGTCTTGTAAATATTCAGCATGAACTCTGTTCTTCCGACGATGATGGTG ATGTGAAGTGGGGGACAGATCTGGGTGTGGTCCTAAAGACAAGATTCTTGATGGGACTGATACCGACAGC AATAGGATCTTTTGAGTATTCAACTCGGAGTAACAGAATTTGTCATCAACGGCATGAATGGCTCAGCCGA GGAAATAGAGCAGCTTTCATCTAAATCCAAGCGTGTCGTCATTTTTTCACTGCTATTATTTTGAAAACTT CTTCAAAACCATTTAAAAAGCATTGACAACTAATTTTATAGATCAAAGAAAGGCAGATGTCTGCAAATAA TTCCCCTCCATCAGCCCAGAAATCTGTGTTCCCTGCAACTGTTTCTGCTGTGCTTCCAGCTCCTTCTCCG TGTTCAAGTCCCAAAACAGGACTCTCAGCTCGGCTCTCTAATGGCAGCTTCAGTGCCCCGTCACTCACAA ACTCTAGAGGCTCAGTGCACACAGTTTCATTTCTACTTCAGATTGGCCTCACACGAGAGAGTGTTACCAT CGAGGCCCAGGAACTGTCTCTCTCTGCTGTCAAAGACCTCGTGTGCTCCATTGTTTATCAGAAGTTTCCA GAGTGTGGATTCTTTGGCATGTATGATAAAATTCTTCTCTTTCGCCACGACATGAACTCAGAAAACATTT TGCAGCTGATTACCTCAGCAGATGAAATACATGAAGGGGACCTGGTAGAAGTTGTCCTGTCAGCTTTGGC CACAGTGGAAGACTTCCAGATCCGCCCTCACGCTCTCTATGTACATTCTTACAAAGCACCTACTTTTTGT GATTACTGTGGTGAAATGCTCTGGGGACTGGTACGTCAAGGATTAAAATGTGAAGGCTGTGGATTAAATT ATCATAAACGATGTGCCTTCAAGATTCCAAATAACTGCAGTGGAGTAAGAAAGAGACGTCTGTCAAATGT ATCTCTGCCAGGACCTGGCCTCTCAGTTCCAAGACCCCTGCAGCCTGAGTGTGTCCCCCTTCTCAGTGAA GAGTCACATACTCACCAAGAGCCCAGTAAGAGAATCCCGTCGTGGAGTGGTCGTCCGATCTGGATGGAAA AGATGGTAATGTGCAGGGTCAAAGTCCCTCACACATTTGCTGTCCACTCTTATGGCCGCCCGACCATATG CCAGTACTGCAAGCGCCTGCTGAAAGGCCTCTTCCGCCAAGGGATGCAGTGTAAAGATTGCAAATTCAAC TGCCATAAACGCTGTGCATCCAAAGTACCAAGAGACTGCCTTGGCGAAGTTACTTTCAATGGAGAGCCCT GCAGCGTGGGGACTGATGCAGACATGCCCATGGATATTGACAGTAGTGATGTGAACAGTGACGGCAGTAG GGGCTTGGATGACTCAGAGGAGCCGTCCCCGCCCGAAGACAAGATGTTCTTCCTCGACCCGACTGATCTC GACGTGGAGCGAGATGAGGAGACTGTTAAGACAATCAGTCCATCCACCAGCAATAATATCCCGCTGATGA GGGTGGTGCAGTCCATCAAGCACACAAAGAGGAGGAGCAGCACAGTTGTGAAGGAAGGGTGGATGGTCCA CTACACCAGCAGGGACAACCTGAGAAAGAGACATTATTGGAGACTTGACAGTAAATGTCTAACATTGTTT CAAAATGAATCTGGATCAAAGTATTATAAGGAAATTCCACTTTCAGAAATTCTCCGTGTATCTTCACCGC AAGATTTCACCAGCATTTCACAAGGCAGTAACCCACACTGTTTTGAAATCATCACCGATACCGTGGTATA CTTTGTTGGTGAGAACAATGGGAGCAGCTCTCATAATCCTGTTCTCGCTGCCACTGGAGTGGGACTAGAT GTGGCGCAGAGCTGGGAAAAAGCAATTCGCCAGGCCCTCATGCCTGTCACTCCTCAAGCAAGTGTTTGCA CTTCTCCGGGGCAAGGGAAGGACCACAATCTGGCTACCAGTATCTCCGTGTCTAACTGTCAGGTTCAGGA GAATGTCGACATCAGCAGCGTTTACCAGATCTTTGCGGACGAGGTGCTTGGTTCTGGCCAGTTTGGCATT GTTTATGGAGGAAAACACAGAAAGACGGGAAGGGATGTGGCTATTAAAGTGATTGATAAAATGAGGTTCC CCACAAAGCAAGAGAGTCAGCTCCGGAACGAAGTGGCCATTTTACAGAATCTGCACCATCCTGGGATTGT AAACCTGGAGTGTATGTTTGAAACCCCAGAACGAGTCTTCGTGGTGATGGAAAAGCTCCATGGAGATATG TTGGAAATGATTCTGTCCAGTGAAAAGAGTCGACTTCCAGAACGGATTACCAAATTCATGGTCACACAGA TACTTGTTGCCTTGAGGAATTTACATTTCAAGAATATTGTGCACTGTGATTTAAAGCCCGAAAATGTGCT GCTTGCATCCGCAGAGCCATTTCCTCAGGTGAAGCTGTGTGACTTTGGGTTTGCCCGCATCATTGGTGAG AAGTCGTTCCGGAGGTCAGTGGTAGGAACTCCAGCCTACTTAGCCCCTGAGGTTCTCAGGAGCAAAGGCT ACAACCGTTCTCTAGACATGTGGTCGGTGGGCGTCATCGTTTATGTGAGCCTCAGTGGCACATTCCCATT CAATGAAGATGAAGATATAAATGACCAAATCCAAAACGCTGCATTTATGTACCCACCAAATCCATGGCGA GAAATTTCCAGTGAAGCAATTGACTTGATAAACAACTTGCTTCAAGTGAAGATGAGAAAACGTTACAGTG TTGACAAATCTCTCAGTCATCCTTGGCTACAGGATTATCAGACTTGGCTTGACCTCAGAGAATTTGAAAC TCGCATTGGAGAACGTTACATTACACACGAAAGTGATGATGCTCGCTGGGAAATACACGCATACACGCAC AATCTGGAATACCCAAAGCATTTCATTATGGCCCCCAACCCAGACGACATGGAGGAGGATCCTTAGTTGT CAGTGCGCCGACCTCAATAAGGAAGGGTTTCACTTCAAGAGCGGATACTTTGCTGCATGGCTGGTGGTCT CTGCAGGCTACCATTGCAAGGCGGCAGCACCGTGAGGAAGTAGAAGGACTTGTGGCATCAGTTCTGTAGC TCATAACGAGTAGGTACATGAAGGGAAACTGAGTAATAAAGTCAAAATGGAACTTCTATAAATTTTTATA GCATAAGCAATAACTGGTTCTGTAATTTTTCCTAATCCTTCACATTAGCACAATGTGAGTTAGCACTTAA TTTATCTCTTTTACCTCTCCTTTTCTGTTATTACATCATTTGTTTTAAAGGCCTATGACTGTCTAGTTAT TGCTTATAAGGTTGATTACTCAACAGGTTTGCAGTTAAGCAGTAACCATGAGGCTAACAGCATACAGCTT GCTGTAAGGACCTTTAGTGTTTGTCCACTGTCGGAGGCATTGCTTCCTCCTTCCTGGACCCGATAAGCAG ACCATTGTACTGCTGGCCTTCCAGTGGATGCGTCTTTCCATATGGCCTGTATGACAGAAGAGTTGTCACA TTTTCCATGTTATAACAGTCACTAGGATAATCGAACAATTGGTGTTACAAATGAATAGAAATAGCTGGTC ATCACTCAGCTCTGCCTCTGAACACGTATGTTCTCAATTGGCACCATCATGTTACAGTATGCCACAGTCG GTGGGCACAACGCTGCATTTAACTAATTTATCCTCACCTGGAGTTGAATAATGTATAGACAAAATCACAT ACTAGAGAGGGAAGCTAACACAGGTCAGTAGATGTCACTGTTATCTGTTTCCTCTTCCCTTGGCCTTTGG ATGGGGTTTCCAATACCTAGTGTTTTTAAATTAGCATTGTCGTTTGAGCAGTTTGGAGGAGTGACTGAAG TCAAACATGAAAACTTAGTTTATTGAGGTGGGACCTAATTACCAGTAGAGCGGATGGAGAAGGAGTAAGA ACGTCTTACACTTCAGCTCCAACAGCCAGCTGTTTTGTAGTAGTTATTAATACAAGCTCAGTATCCCTAT CTGAGCTGCTCTACACCAGGAAGGTTTCACTGCCAGATACTGAAATGTTTGCACACTCTACTAGTTGAGC ATCCCTGACCTACAAATCCGAAATCCTCCAAATTCAAGAAACTTTGGAGTCCTTCAGATTTCCACATTAG TGATATTCCCACCTGTGCTTTACCCAAAGAGAAAAAGATCCAGGACCATCCTCTCACAAGAAATATAGTA GCTGTTTGTTTCTTCATTCCTCACTAGTCACAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5342428 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5342428 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7244 same embryo
	EMAGE:7243 same embryo
	EMAGE:7247 same embryo
	EMAGE:7242 same embryo
	EMAGE:7245 same embryo
	EurExpress:euxassay_004341 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source