EMAGE mouse gene expression spatial database

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EMAGE:7269

Zfp828 zinc finger protein 828 ( MGI:1196398)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004361. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004361_01	euxassay_004361_02	euxassay_004361_03	euxassay_004361_04

euxassay_004361_05	euxassay_004361_06	euxassay_004361_07	euxassay_004361_08	euxassay_004361_09

euxassay_004361_10	euxassay_004361_11	euxassay_004361_12	euxassay_004361_14	euxassay_004361_15

euxassay_004361_16	euxassay_004361_17	euxassay_004361_18	euxassay_004361_19	euxassay_004361_20

euxassay_004361_21	euxassay_004361_22	euxassay_004361_23	euxassay_004361_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7269_wholemount_strong.wlz

7269_wholemount_moderate.wlz

7269_wholemount_weak.wlz

7269_wholemount_possible.wlz

7269_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7269_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5218
Entity Detected:	Zfp828, zinc finger protein 828 ( MGI:1196398)
Sequence:	sense strand is shown >T5218 CCGCCATTCCCGAGGCCGCACAGCCAGCCCAGAGTTTCCTGTGGGCGTTTCCTCCTCTTCACTTCAGGAC GCCTCGCCGGATGCACTGTTGAAGTCTTTCAAGTGCAGTTCTACGGCGTGTGACAGAAGATGGAAGTGTG TCAGGAATTGAGGAAACCAGCGCTAAGCCTGGAGTGTGGGCACTGCAGTTTCAGAGGCACCGATTATGAG AATGTGCAGCTCCACATGGGCTCCATTCATCCTGAGTTCTGTGATGATATGGATGCCGGGGGCCTGGGCA AGCTCATCTTTTACCAGAAGAGTGCAAAGCTCTTCCATTGCCATAAGTGCTTCTTCACCAGCAAGCTGTA CGCCAATGTGTACTATCACATCACGGCCAGACACGCAGCCTCGGACAAGTGGAGTGAGCAGCCGAAAGAG CAGCCGAGCAAAGACACCGAGTCTGGGAAAAGCCCTTCCCCTCCTGAGCGGCAGAACCCAGCCTTTGACC CAGCAGAGGCGAGGCCCACTCCAGCCCTCCCCATGGAAGCACAGAAAACGAGCCCGAGCTTGTGTCCAGA GTCACAGGCTTCTGGTCCTCCAGTTCTGGAGCCTCAGGGCGCTGGTCCTCTTATTTCGCCCGAGCCCCAA GCCCCTTGTCTTCCTGCTGAGGCCTCAAAAGCTGCTCCTGTTCCTTGCCCTGAGCGTGTAGACCCACCCT GTGAGCTTCCTGAGCTGGAGAAGCCTGAGCGAGGCCCTTCTCCTGAGTCAGTCAAGTCTGCTCTTGTCAG CTCCAAACCCCCAAAGCACTCTTCCTTTGCAGACACAGGGGCTGCCCCTTCAGCTTTGTCTCCAGAGTCA CCAGTACTGGCCACTTCCCCTGAACCTTGGGGACCATCCCTCAGTGCATCTCCAGAGTCTCGGAAGCCAG CGAGGACTGCCTCCCCTGAGCCAAGGAAGCCATCCCCAGCAGAGTCTCCTGAACTTTGGAAGCCATTCCC TGCCATCGCTTCAGAGCCTCGGAGACCAACCCCAGCAGTGTCGCCAGGTTCCTGGAAGCCAGGGCCGCCT GGTTCTCCTAGGCCTTGGAAATCCAGTCCTTCAGCAACATCAGGACCGTGGAAGTCATCTAAACCTGTTC AACCTATGTCTCCTGGACCTTGGAAACCAATACCTTCTGTATCACCTGGGCCTTGGAAACCTGCCCCATC TATGTCCACTGCATCCTGGAAGTCTTCAGTCTCATCTGGTTCCTGGAAAACACCACCCACATCTCCAGAG TCATGGAAGTCTGGCCCACCTGAACTCCGGAAGACAGCCCTTCCCTTGTCTCCTGAGCACTGGAAGGCAG TGCCTCCCGTGTCTCCTGAGCTCCGCAGACCAGGCCCACCACTTTCCCCAGAGATCCGAAGTCCAGCAGG TTCTCCAGAGCTCAAGAAGCCTTCAAGCTCACCAGACCTTTGGAAGGTTTCTCCTGACCAGCGGAAAACT TCTCCTGCTTCACTTGATTTCCCTGAGCCCCAGAAAAGTTCTTGTGGTAGTCCTCCTGATCTCTGGAAGT CTTCCTTCATTATGGAGTCTCAGAAACCTAACGTCTTCTCTGAGACGAGGAAGCACACTGCTTCGGGCTC ATCTGAGTCACCGAAGGTAGCGTCAGACATATGGAAGCCTGTCTTGTCTATCGATGCTGAGCCTAGGAAG TCCACACTGTTTCCTGAGCCCACCAAAGCAGTCCTTCCTGCTTCTCCCGAGCCACGGAAACGCGCACTTT TCCCAGAGTCCCGGAAGCACGTGTTTCTACCTGAACTTCCCAAATCCGCTGTGTTCTCAGACGCCCAGAA GGCCCCTGAGCTTAGTGAGGAGATACAACTTGAAGCTGTGGATAATGCAAAATGCGATAGTCTGGCCCAG GAAGGGCTTCTGGCCACCCCCAAGAAACTGTTAGACGAGGCTTTATCTCCTTCTTCAAAGAAGCTCAAGA AGGACAGCCAGGAGAACTCGGATGCCGAGCTCAGTAGCAGTGAATACATCAGAGCAGATTTAGACACGTT GGACACGAAGGGCCAGGAGTCAAGCAGCGATCAGGAACAGGTGGATGTGGAATCGATTGATTTTAGCAAA GAGAACAAAATGGAGATGGGTAGTACAGAGCAAGCCAAAAATGTGCTTCAGTTCACCGAGGAGAAGGAGG CCTTCATCTCTGAGGAGGAGATCGCAAAGTACATGAAGCGTGGGAAAGGGAAATACTACTGCAAAATCTG TTGCTGTCGGGCCATGAAAAAGGGCGCTGTCTTGCACCACTTGGTTAATAAGCACAATGTCCACAGTCCA TATAAGTGCACAATTTGTGGAAAAGCGTTTCTTTTGGAGTCTCTTCTTAAAAACCACGTAGCAGCCCACG GGCAGAGTTTACTTAAATGTCCACGCTGTAATTTTGAATCAAATTTCCCAAGAGGCTTTAAGAAACACTT AACTCACTGTCAGAGCCGGCATAATGAAGAGGTGAATAAGAAGCTGATGGAAGCCCTGGAGTCGCCATTG GAGGAGCAGCAGATTTGATCTTCAGACAGTGGCCCTTGCTCTACAGTGTTGCTTGCCAGACTCTTAGGTT GTTGAGTAGCCTAGATGGACCAGTAGCCAGTATGTGTGTAGTGGTGTATCCTGGTCTGTAAGTAGTGTTA CAAAAAATAAGTACTTGGTTATTTTTTCATATGATCCGTTGTTTATGTGGATGGCTATTTTATAAGTCTT AATACATTTCTGTTATAGATTCCAGATGGATATAACTCATTTTCCTTAAGTTTTATTTTTGTTATAATAC AAGGAGTGGGGGAAAATAGGCAGGCCATTTAATAAACAAGTAAGTTTAAACCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5355869 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5355869 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7274 same embryo
	EMAGE:7272 same embryo
	EMAGE:7273 same embryo
	EMAGE:7271 same embryo
	EMAGE:7270 same embryo
	EurExpress:euxassay_004361 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source