EMAGE mouse gene expression spatial database

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EMAGE:7271

Erlin2 ER lipid raft associated 2 ( MGI:2387215)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004373. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004373_01	euxassay_004373_02	euxassay_004373_03	euxassay_004373_04

euxassay_004373_05	euxassay_004373_06	euxassay_004373_07	euxassay_004373_08	euxassay_004373_09

euxassay_004373_10	euxassay_004373_11	euxassay_004373_12	euxassay_004373_13	euxassay_004373_14

euxassay_004373_15	euxassay_004373_16	euxassay_004373_17	euxassay_004373_18	euxassay_004373_19

euxassay_004373_20	euxassay_004373_21	euxassay_004373_22	euxassay_004373_23	euxassay_004373_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7271_wholemount_strong.wlz

7271_wholemount_moderate.wlz

7271_wholemount_weak.wlz

7271_wholemount_possible.wlz

7271_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7271_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5211
Entity Detected:	Erlin2, ER lipid raft associated 2 ( MGI:2387215)
Sequence:	sense strand is shown >T5211 GTTGCACGGGGGTTCGGCTGTGTACGGAGCGCCTGGAGGGACAGCCTGGATACAGGTTCACTGATGGCTC AGTTGGGAGCTGTTGTGGCCGTGGCTTCCAGTTTCTTTTGTGCATCTCTCTTCTCAGCTGTGCACAAGAT AGAAGAGGGACATATTGGAGTATATTACAGAGGTGGTGCCCTGCTGACCTCCACCAGTGGCCCGGGTTTC CATCTCATGCTCCCGTTCATCACATCCTATAAGTCTGTACAGACCACTCTCCAAACTGATGAAGTGAAGA ACGTACCATGTGGAACCAGTGGTGGTGTGATGATCTACTTTGACAGAATTGAAGTGGTGAACTTCCTGGT CCCAAATGCAGTGTATGATATAGTGAAGAACTATACTGCAGACTATGACAAGGCCCTCATCTTCAACAAG ATCCATCATGAGCTTAACCAGTTCTGCAGCGTTCATACTCTTCAGGAAGTCTATATCGAGCTGTTTGATC AAATTGATGAAAACCTCAAGTTGGCTTTGCAGCAGGACCTGACTTCCATGGCCCCTGGGCTGGTTATCCA AGCTGTGCGAGTGACAAAGCCCAATATACCTGAGGCAATCCGCAGGAACTATGAGCTGATGGAAAGCGAG AAGACGAAGCTTCTCATTGCAGCCCAGAAGCAGAAGGTGGTGGAAAAGGAGGCAGAAACAGAGAGGAAGA AGGCCCTCATTGAGGCAGAAAAAGTGGCACAGGTTGCAGAAATCACCTATGGGCAAAAGGTGATGGAGAA GGAGACAGAGAAGAAGATCTCAGAAATTGAAGATGCTGCGTTCCTGGCCCGGGAGAAGGCGAAGGCCGAC GCTGAGTGCTACACAGCGCTGAAGATCGCAGAAGCAAATAAGCTCAAGCTGACTCCAGAATACCTGCAGC TGATGAAGTACAAGGCCATTGCTTCCAACAGCAAGATTTACTTCGGCAAAGACATCCCCAACATGTTTAT GGATTCCGCAGGGGGGCTGGGCAAGCAGTTTGAGGGGCTGAGCGACGACAAGCTGGGCTTTGGCCTAGAA GATGAGCCCCTCGAGGCACCCACAAAGGAGAACTGAGGAAACACTGTCTGCAAGCTCTGCTCGGGCAGCT TAGAGAGAGCTGTATTCTTTAAGATGAGACAGAGCAAAGCGCTCCTCCTTTCCACACTACCTTCCTTGAC TCTTCTTACTGTGGTTAAAAAGGAAGAAATGGACACAAACTTACCCCCTTCTGGGAAGGGAGAGCAGATG GAGAGTTGTTTTTTGGGTTTATTTTTAATTCAGGTAAGTAAGTTGTATGACTTCTGAGAAGGTGTATGCA CCGTAGATTTGACCTCTGACCTGCAGACACCAACATTGTCACTTTGAAGCTGGTTTAAGTGGAGCTACTG TCAGTATGAAGAGGGAGAGTGTGTGCTGCCTCCTCGTGCTTGAATTCCTTCAGGGAAAAGTGTACTCCAC AGTTCTCTCCCTTGCCTCTAGTGTAGGCAGTGTCTGCGTGTGGGGCTCGTGACAGAAGGCCGTCTGCTGC GGAACATGAGCTGCAGAGAGCGTTGGCCGGCTGGGCTTTTTGACTGAGTGGATTACTTGAGAGTTAAGCT GTCTTGAGCCCTTTTTAGGAAGAACTTGGTGCTAGGTTTTGCAAGGTTTTCTACACACTGTACTCTGCTC TAGTGTTTGTTGGCTACATCTCACCGCAGCAGGGCTTGGTCACACCACACACTCCTTTTCCGTACTTTGA CCTGATCTGTGATTTCATTTCTTCTTGAATAATCTATTCATGAGTTGCACTCAGCGTTAAGATGGGAACA AACAAGTGCTGTTAGCTGATGACGTAGCTCCTTATACCCCTTAGCACTGTGGTGCTGTGTGGCTAATTAT GCGTATGCTTTTGAGACCAAACATCTTTATCATTATGGAGATTCTTCATTGAAGAGCCCTTAACACTGTG GAGAAGGGCCCAGCCAGATGACACCCAAGTAGTAGTGCCTGTGGCCTGTGCTGGGGCTTTGTCTGACACT GATGAAGAGAGCAGGCAGCCACTTGAGAGTCGGCTCCAGTGAGTCACCCTAGGAAACTGAGAATGCGAAG AATAGATATGAGAGAAAGGGATTTCTTATCCTGAAATTGCACTGGGGGTGGGGCTCTACCATGGCCTGTG AGTGCACACAGAATGCCTCTGTGGAGGGCAGCTCTGCAGGTAATCTGCAGACATGGCAGTACCCTGTGCA ACCATGACTGGCTCTAGCTTAGGACTTGGCCTTGTTAGCTGGTCCCCTACCTCATCCTCCCCCCACACAA AGCACCTACTGTTCTCTCTTAGGTGACTACTATAAATGGTATTTTCTGGCATCAATTCCCACCTCAGTTT TGGTTTTGTAAGTCGGGCCAGTTTGCTCCTAAGTGGCACCAGACTTGTCAGGTATTTGGGAAGCATTCAG CCGACCCAAAAAGAGGCAGGGTTCACTGTGCTTACTTCAGATGTTCCCTTCTCTGTCCTGACTCCTCAGG CCACTGACCCTGGCCACACTGTACAAACTACAAAATGTTCCTGAAAAGGACATTTTAATGTGCTCAAAAG CTCTTGCAAAAGTGGGTTTTTTTCCCCAAGACCAACTCATCTTCTTCTCATTTGTTGCTGCTAACCACTT GTTGAGAGCAACGTGCTATACCCAGCATCCTCTCTTGTACGTGCACCTGAGAAAACACTACTTCAGTGGA GTCGGTGCAGGAGGGAGGGTACCCCGCCATCCAGCGCCCTCCTAGCCCGAGAGGCTCTGTAACTAGCATT CTGAGAGCTCATCCCTCCATTACAAAGAGCCACAGTAAAGTCCTGCTGCAGCTGCTCCTTCCCTGCCCCT TTAATGTCACTTCTTTAACAGAACAGAAATGTCCCCATGTCATAGCATAAATTCAGTAGCTATTGGTATC TGTCCCAGCAGTAAAATCATGGAACTCAGATGTCTTTTTAGCATGGGATGCCTAGCCCATCTGTCTTTAT GACCTTGTTTTTTGTAATACTATAAAATCTGACTTAGGCATTTGAATTCTAAACATGTAAAATGTGATAA GCCTGCAGTTTTGTAGGCAGTGAATTCATAGCTGCTATTTTTAAGTAGAACTTCTATCAAAATACGTTAA CCGTTTGTAAAATTCAGTTTTTGTAGGACTTTCCCAAGGCCCAGCCACCTTGGTAGAATGCTTCTCACTC ACTAAATGTTGCAGAAGCAATTTATATTCCATATAGGTTTTTAATCACTTTTCAATATATGGTTAGAATG TTTGTAAGGAAGCCTAAGTTTAATAATTTTTATATAACTAAAAATAGGTGTGGAGGACTCAGTGTGGGTA CTGAGGAGGAATGAAGTGCTCTGAAAAGGGAGGTGTATAAACGGCCTGTGGGGCCGTGTGTCTTGTGAAA GTGAGATAGCCGTGCTTACTGACCTGGGCTGTCGTCAGCTGGCCGTCGGTAAACTACCTGGACAATAGCC CCTCTGTCTGGGAACTTTACCTACTTCCTTGTCCTCAGTGGGCTTCTAGCCACTGTTTGTTTCCTTATAA AAGCTGTAATGGGCAATCATGTGTTTGTACTTCCATTCCTTTTTATCTCTACTTCTGTGTAAACTGGTGA TTGAATAGTTAAAGCAATTTTTTCAGTGTGCCCCAAGGGCATTAATGAGCCTTTATAACTGAGAAATGAT TCTTGTTATAGTAATTATTCCATAAATGATACCACTAGATAAATTACCTTGGGTTAATAGCTCCAGGATT TGTTTCAGACAACAAAAAAAGGTCTCAATGTGAATATACTTACATTTTGGATTTAATTTCAGTCTTGCTA AATAAAATGTTTTTGTCTTTTTTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5355446 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5355446 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7274 same embryo
	EMAGE:7269 same embryo
	EMAGE:7272 same embryo
	EMAGE:7273 same embryo
	EMAGE:7270 same embryo
	EurExpress:euxassay_004373 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source