EMAGE mouse gene expression spatial database

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EMAGE:7273

Ints12 integrator complex subunit 12 ( MGI:1919043)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004366. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004366_01	euxassay_004366_02	euxassay_004366_03	euxassay_004366_04

euxassay_004366_05	euxassay_004366_06	euxassay_004366_07	euxassay_004366_08	euxassay_004366_09

euxassay_004366_10	euxassay_004366_11	euxassay_004366_12	euxassay_004366_13	euxassay_004366_14

euxassay_004366_15	euxassay_004366_16	euxassay_004366_17	euxassay_004366_18	euxassay_004366_19

euxassay_004366_20	euxassay_004366_21	euxassay_004366_22	euxassay_004366_23	euxassay_004366_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7273_wholemount_strong.wlz

7273_wholemount_moderate.wlz

7273_wholemount_weak.wlz

7273_wholemount_possible.wlz

7273_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7273_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5196
Entity Detected:	Ints12, integrator complex subunit 12 ( MGI:1919043)
Sequence:	sense strand is shown >T5196 CCACGCGTCCGACCGAGTGGCGGGGCGGAGCGGTGTTTGCAATGGCTGCTACTGTGAACTTGGAACTTGA TCCCATTTTTCTGAAAGCACTAGGTTTCTTACATTCAAAGAGTAAAGATTCTGCTGAAAAGCTAAAAGCG TTGCTTGATGAGTCTTTGGCTCGGGGCATTGATTCCAGTTACCGTCCAACTCAAAAGGATGTGGAACCAC CCAAAATTTCAAGTACAAAATCACTTTCTATTAAGCAAGAGCCCAAAACATCATCTAGCCTCCCTTCCGG CAGTAGTAACGGCAAAGTCCTCACAGCTGAGAAGATAAAGAAGGAGGCTGAGAAGAGACCTGCTGATAAG ATGAAAGATGTCACTGAAGGAATTGATGTTCCAAAGAAACCTAGACTGGAGAAGCCAGAGACACGATCCT CTCCCATTACTGTCCAAACCAGCAAGGATTTGAGTATGGCTGACCTCTCCAGCTTTGAGGAGACTAGTGC TGATGACTTTGCCATGGAGATGGGGTTGGCCTGTGTTGTTTGTAGACAAATGACCGTGGCATCTGGAAAC CAACTGGTAGAATGTCAAGAGTGCCATAACCTCTACCACCAAGACTGCCATAAGCCCCAGGTGACAGACA AAGAAGTGAATGACCCTCGCCTGGTGTGGTACTGTGCCCGGTGCACTAGACAGATGAAGAGAATGGCTCA GAAAACTCAGAAGCCACCACAGAAACCAGCGCCCACTGTTGTTTCTGTGACTCCAACTGTCAAGGATCCA TTAGTCAAGAAGCCAGAAACCAAACTTAAACAAGAGACAACCTTCCTAGCATTCAAGAGAACCGAAGTTA AGCCATCCACGGTTATTTCAGGAAATTCTTCTAGTAACAACGTGTCCTCTTCAGTAACTAGTGGCCTAAC TGGATGGGCAGCTTTTGCAGCCAAAACTTCTTCAGCTGGTCCATCAACAGCAAAATTGAATTCAGCAGCA CAAAACAGTAGTGGAAAACCTGCTGCCTCATCCTCTAACCAGAAGCCTGTGGGTTTGACTGGTCTGGCAA CATCATCCAAAGGTGGAATAGGTTCCAAAATAGGTTCTGGTAACAGCACTAGCCCCTCTGTCCCGTTGAA ACCGCTTCCACCACTAACCTTAGGCAAGACTGGCCTGAGTCGCTCAGTGAGTTGTGATAACGTCAGCAAA GTGGGTCTTCCTAGTCCTAGTAGTTTAGTTCCTGGAGGCAGCAGCCAATTGAGTGGGAATGGAAATAGTG CTACAACGGGGCCCAGTGGAAGTACCACCAGCAAAGCCACATCAGAGACCAGCAGCAGTACCTCAGCATC TCTTAAAGGTCCAACTTCTCAAGAATCACAGCTCAACGCAATGAAGCGATTACAGATGGTCAAGAAGAAA GCTGCCCAAAAGAAACTCAAGAAGTAATATGGCCAGATAGATTTTTATATCATATTACTCTAAAACTTAA AAAACAAAACAAAACAAACAAAATAAAACCCTTACTGGGACAAAAGTTGTAATGTACTGTACCTTAATAA AAATCTTGATCAAATCTCTATTGTTTGTTACATTAAAATGCTAATATTATTTAGCCAAACTTGGATCTAT TTATTTCAGTCTGTTTAGCATTTTTAATTTCCATAGTGTGTGAAAATAGTGATTATAATGACTGATGCAT GACAAGAGCTTCAAGAAATTGATGGCGCATACAGATGAAAAGAAATGGCTCACCTGGTACCTTATGGGTG GCAGGGACAACTCAATAGAAAGTGTTCCAGCGTTTTATAAAATCTGAAATGTTCAGTGTCAGTCATTGTC CTCATGTCAGCTAGTATCTCTCTCCAATTACCTCCTCTTTTATCAAAATTAATTTTGAAAGTGATTTCCC ATAGCTCCCTATCATGTCTTTAGCTCTCAACCACCCCTTATCAATTACTTAGGGCGTTATTTATTCCAGT AGATTATGAATGCTCATACAGTATACTTTCTCACGTTGCCCACTTTCCGTACCTGGCCAGTGTGTGGATA TGTGCAGATATGTACATTTAATAAATGCTGACTTGGCTCATCCTGGTTGCACATTCTGAGAATCATTGAG AAGAGTATCCATTTCCAAGAGGCTTGGCAGATGGGCATTTATGACTAGTTTGCACCCAGCTTCTTCCTCA TGGTGAAAGGACAAGCTGTTTTTAATACTTCAAGAACGTTTGGTGTGGTTTATCTTTGTATGAAAGAGGC TCCTACCTAATGATTAGTCCTTGAGGTTTCTGTCAGAACGTGCTCATATGCTTTGAATATTGTTAGGATA TGGTTTGTCTTAAGGTTTTATGTGTTAGGAAGCATAATCCCCAATATGGCAATGGAGTTGGTGGTTGACA GGTGGAGCCTGTTGGGGGAAGAGTATGCCCTTGGGAAGGATTAATTAGTCCTCATATGTCCACTGACTAA TTCTGGCCAAGAGTGGAGTTATAAAAGGCTATCCCAGAATTGACCCTTGGGCAAAATCTCCTCTGAACTC TTGCCGTGATGTGATCCTATATACATGGTCCAAGCCATTGTCTTGTAAGCTAATGAATTACCCATTAGTT TACATATATATATGTATATATGTACATACCCCAACAGAGAATACTTTCTGTATCTGACCAGTAAATGTAT TATAGAAAAATAGCTTCCTTTCTTTTTTATAATCAATGAATACCTCATTATTTCCCAAAAATGAATCTTG TGATTTCCTATATTCATTGAGTCAAATTGCTTTAAATCTAATCTCAGTGTATCTTTATCCCTAGATGTTC TAACATCATTATTCTAAAAATAAATTGATAATCATTGTAAAAGAGAAAACTTTTAAATTAAGCTTGGTCT TTTTCTATGGAAGTATTTTAAACTGTTAAATAATGGCGTTAAATTGTTCATAATAAATTAGAAACCAATA TTGAAATAAACTCAAAATTTAGAGATGGAATAAATATCTATTTATATACTTAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:5353058 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5353058 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7274 same embryo
	EMAGE:7269 same embryo
	EMAGE:7272 same embryo
	EMAGE:7271 same embryo
	EMAGE:7270 same embryo
	EurExpress:euxassay_004366 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source