EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7279

Mical1 microtubule associated monoxygenase, calponin and LIM domain containing 1 ( MGI:2385847)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004335. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004335_01	euxassay_004335_02	euxassay_004335_03	euxassay_004335_04

euxassay_004335_05	euxassay_004335_06	euxassay_004335_07	euxassay_004335_08	euxassay_004335_09

euxassay_004335_10	euxassay_004335_11	euxassay_004335_12	euxassay_004335_13	euxassay_004335_14

euxassay_004335_15	euxassay_004335_16	euxassay_004335_17	euxassay_004335_18	euxassay_004335_19

euxassay_004335_20	euxassay_004335_21	euxassay_004335_22	euxassay_004335_23	euxassay_004335_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7279_wholemount_strong.wlz

7279_wholemount_moderate.wlz

7279_wholemount_weak.wlz

7279_wholemount_possible.wlz

7279_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7279_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5137
Entity Detected:	Mical1, microtubule associated monoxygenase, calponin and LIM domain containing 1 ( MGI:2385847)
Sequence:	sense strand is shown >T5137 CCACGCGTCCGGTCTTCCTCTGTGCTCTGCGCTGGCGCTGCGGTCCAAACTCTTTGGAACTCCCCGGTTT CTGCCACCAGGCATCTCTTGCTTTCCCTGCGCCACCCCTTCCTCTGCCCCTAGGCCTGGGACACCCTTCC GACAGGAAGCGGCTCTTCCAGCCCCAGAAACGCTGACACTGGGTTTTCTCCCAGCTGCTCCTGCTGTCAA CAGGGGCCCTACATGGCATCACCCGCCTCCACCAACCCAGCACATGACCACTTTGAGACCTTTGTGCAGG CACAGCTGTGCCAGGATGTACTGAGTAGCTTTCAGGGGCTCTGTAGAGCCCTGGGAGTGGAGTCTGGTGG GGGATTGTCCCAGTACCACAAGATCAAGGCCCAGCTCAACTACTGGAGTGCCAAGTCACTGTGGGCCAAG TTGGACAAGAGAGCGAGCCAGCCTGTGTACCAGCAAGGCCAGGCCTGTACCAACACCAAGTGTCTCGTGG TAGGTGCCGGGCCTTGCGGACTTCGGGCTGCTGTGGAGTTGGCACTGTTAGGTGCCCGAGTGGTACTTGT GGAAAAGCGTATCAAGTTCTCTAGGCACAATGTGCTCCATCTCTGGCCCTTCACCATCCATGACCTTCGG GCACTTGGGGCCAAGAAGTTCTACGGGCGCTTCTGTACTGGCACCCTGGACCATATCAGCATCCGACAGC TTCAGCTGCTTCTACTGAAGGTGGCCTTACTGTTGGGGGTGGAGATTCACTGGGGCGTCAAATTCACTGG CCTCCAGCCCCCTCCCAGAAAAGGGAGTGGCTGGCGTGCTCAGCTCCAGCCCAACCCCCCAGCCCAACTG GCCAGCTATGAGTTTGATGTCCTCATCTCAGCTGCAGGAGGCAAATTTGTCCCTGAAGGCTTCACTATAC GAGAGATGCGTGGCAAACTGGCCATTGGCATCACAGCCAACTTTGTGAATGGGCGCACGGTGGAGGAGAC ACAGGTGCCGGAGATCAGTGGTGTAGCTCGAATCTACAACCAAAAATTCTTCCAGAGCCTGCTCAAAGCC ACAGGTATTGATCTGGAGAACATTGTATACTACAAAGACGAGACCCACTACTTTGTGATGACAGCCAAGA AGCAGTGCCTGCTGAGGCTGGGGGTGCTGCGCCAGGACTTGTCAGAGACTGATCAGCTGCTGGGCAAAGC TAATGTGGTACCGGAGGCTCTGCAGCGCTTTGCCAGAGCAGCGGCTGACTTCGCCACACATGGCAAGCTT GGGAAACTGGAGTTTGCTCAGGATGCACGCGGGCGGCCTGATGTGGCGGCCTTCGACTTCACAAGCATGA TGCGGGCAGAGAGTTCCGCTCGTGTCCAAGAAAAGCATGGTGCTCGCCTACTGCTGGGGCTGGTGGGGGA CTGCCTAGTGGAGCCCTTCTGGCCTCTGGGCACTGGAGTAGCCCGAGGCTTCTTGGCAGCCTTCGATGCA GCCTGGATGGTGAAGCGGTGGGCAGAGGGCGCTGGGCCGCTAGAGGTGTTGGCTGAACGCGAGAGCTTGT ACCAGCTTCTGTCACAAACATCCCCCGAGAATATGCATCGAAATGTAGCCCAGTATGGGTTGGACCCTGC CACCCGATACCCCAACCTGAACCTCCGGGCTGTAACCCCCAATCAGGTACAGGACCTCTATGATATGATG GACAAGGAGCATGCTCAGAGGAAGAGTGACGAGCCGGATTCCAGGAAGACAACCACAGGGTCCGCAGGCA CGGAGGAGCTTCTGCACTGGTGCCAGGAGCAGACAGCTGGCTTTCCTGGAGTCCATGTCACTGACTTTTC TTCCTCGTGGGCTGATGGGCTAGCTCTGTGTGCCCTGGTACACCACCTACAGCCTGGCCTGCTGGAACCC TCGGAGCTGCAGGGCATGGGAGCTCTAGAAGCCACTACCTGGGCACTGAGGGTGGCAGAACATGAGCTAG GCATCACACCAGTGTTGTCTGCACAGGCAGTCATGGCTGGCAGTGACCCACTGGGTCTCATTGCCTACCT CAGCCACTTCCACAGTGCCTTCAAGAACACGAGCCACAGCTCAGGCCTTGTCAGCCAGCCCTCTGGAACC CCCAGTGCTATACTTTTCCTTGGCAAACTCCAGAGGAGCCTACAACGGACCCGCGCCAAGGTAGACGAGG AGACTCCGAGCACTGAGGAGCCGCCTGTCTCCGAGCCCAGTATGTCTCCAAACACTCCAGAGCTCTCTGA ACACCAGGAGGCTGGGGCAGAGGAGCTGTGTGAACTCTGTGGGAAACATCTGTACATCCTAGAACGCTTC TGTGTGGATGGCCATTTCTTTCACCGGAGCTGCTTCTGCTGCCATACCTGTGAGGCCACATTGTGGCCAG GTGGCTATGGGCAACATCCAGGAGATGGACATTTCTACTGTCTCCAGCACCTACCCCAGGAGGACCAAAA GGAGGCTGACAACAATGGAAGTCTGGAGAGCCAGGAGCTCCCAACACCAGGAGACAGCAACATGCAGCCA GACCCCTCCTCTCCTCCTGTGACGAGGGTCAGCCCTGTCCCAAGCCCCAGCCAGCCTGCACGTCGGCTGA TCCGGCTCTCCAGTTTAGAACGCCTACGGCTGTCCTCCCTGAATATCATCCCTGACTCAGGAGCGGAGCC TCCTCCCAAACCCCCAAGGAGCTGCTCAGACTTGGCCCGAGAATCCCTGAAGAGCAGCTTTGTGGGCTGG GGTGTGCCAGTCCAAGCACCACAAGTTCCTGAAGCCATAGAGAAAGGGGATGATGAGGAGGAGGAAGAGG AAGAAGAGGAGGAGGAGGAGGAGCCACTGCCACCTTTGGAGCCAGAGTTGGAGCAGACTCTGCTGACCTT GGCCAAGAACCCAGGTGCCATGACTAAGTACCCAACGTGGCGTCGAACCCTCATGCGCCGTGCCAAAGAG GAGGAGATGAAGAGGTTTTGCAAGGCCCAGGCCATCCAGCGAAGACTGAACGAGATTGAGGCCACTATGC GGGAGCTGGAGGCCGAAGGCACGAAGCTGGAGCTGGCCTTGAGGAAAGAGAGTAGCTCTCCAGAACAGCA GAAGAAACTCTGGCTGGATCAGCTGCTACGGCTCATTCAGAAGAAGAACAGCCTAGTGACTGAAGAGGCC GAGCTCATGATCACGGTGCAGGAGCTGGATCTGGAAGAGAAGCAGCGGCAGCTAGACCACGAGTTGCGGG GCTACATGAATCGGGAAGAAACTATGAAGACAGAGGCCGACCTGCAGTCTGAGAACCAGGTCCTAAGGAA GCTGTTGGAGGTGGTGAACCAGCGGGATGCTCTGATCCAATTCCAGGAGGAACGGAGGCTCAGAGAGATG CCTGCATAGATGGGAGCCCAGGGCTAGAAGATGATGGTTAGCTGCCTCCCTGCCCCGTAAGCAAGACACC ATCACAAGGGCTGTACCTCCGCCATCTGGACTGCTTAGTCAGAGGCTTCGTTGTTTACAATAAAAGGATT ATGACCATGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5343552 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5343552 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7280 same embryo
	EMAGE:7277 same embryo
	EMAGE:7276 same embryo
	EMAGE:7275 same embryo
	EMAGE:7278 same embryo
	EurExpress:euxassay_004335 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source