EMAGE mouse gene expression spatial database

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EMAGE:7326

Napa N-ethylmaleimide sensitive fusion protein attachment protein alpha ( MGI:104563)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004412. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004412_01	euxassay_004412_02	euxassay_004412_03	euxassay_004412_04

euxassay_004412_05	euxassay_004412_06	euxassay_004412_07	euxassay_004412_08	euxassay_004412_09

euxassay_004412_10	euxassay_004412_11	euxassay_004412_12	euxassay_004412_13	euxassay_004412_14

euxassay_004412_15	euxassay_004412_16	euxassay_004412_17	euxassay_004412_18	euxassay_004412_19

euxassay_004412_20	euxassay_004412_21	euxassay_004412_22	euxassay_004412_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7326_wholemount_strong.wlz

7326_wholemount_moderate.wlz

7326_wholemount_weak.wlz

7326_wholemount_possible.wlz

7326_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7326_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6432
Entity Detected:	Napa, N-ethylmaleimide sensitive fusion protein attachment protein alpha ( MGI:104563)
Sequence:	sense strand is shown >T6432 GTGTCCGGGGCCTGTGAGTCGTCGCCAGGCTGTGTCGGGCCTGAGCCGAGCAGCTGCGCGACGTCATGGA CAACTCCGGGAAGCAGGCTGAGGCTATGGCGCTGCTGGCTGAAGCGGAGCGCAAGGTGAAGAACTCGCAG TCCTTCTTCTCCGGCCTCTTTGGAGGCTCATCCAAAATAGAGGAAGCATGCGAGATCTATGCCAGAGCGG CGAACATGTTCAAGATGGCCAAGAACTGGAGCGCTGCTGGGAACGCTTTCTGCCAGGCTGCCCAACTGCA CCTACAGCTCCAAAGCAAGCACGATGCAGCCACCTGCTTTGTGGACGCTGGCAATGCTTTCAAGAAAGCT GACCCCCAAGAGGCCATTAACTGTCTGATGAGAGCAATTGAGATCTATACAGACATGGGCAGATTCACAA TCGCAGCCAAGCACCACATCTCCATCGCTGAGATCTATGAGACAGAACTGGTGGATGTAGAGAAGGCCAT CGCCCACTATGAGCAATCTGCAGACTACTACAAAGGAGAAGAGTCCAACAGCTCAGCCAACAAGTGTCTG CTGAAGGTGGCTGGCTACGCCGCACAGCTGGAGCAGTACCAGAAGGCTATCGACATCTATGAGCAGGTGG GGACCAGTGCCATGGACAGTCCCCTCCTCAAGTACAGCGCCAAAGACTACTTCTTCAAGGCGGCCCTCTG CCACTTCTGCATTGACATGCTCAACGCCAAGCTCGCTGTTCAGAAGTATGAGGAGCTCTTCCCGGCCTTC TCGGATTCCCGGGAATGCAAGCTGATGAAAAAGCTGCTGGAAGCCCATGAGGAACAGAATGTGGACAGCT ACACAGAGGCGGTGAAGGAATATGACTCCATCTCCAGACTGGACCAGTGGCTTACCACCATGCTTCTGCG CATAAAGAAGACCATTCAGGGTGACGAGGAGGACCTGCGCTAGGCCTCCAGCCACTGCCTTCCACCCATC TGTCTATCACAGAGGTGGAGCCAGCACCTCCCACCAGGAGTCTGAAGGAGTGGGTGCAATGTGGGCCACA TTCTCCAGCACCAGGCGAGCTCTCCTGGATGGCTGGTCACCCGCTGCGTCCTTCACCCTGCCGTTTATTT AAGCCCCCAGAGGTGCCCTCCTGCCCTGGAAAGTCCACCTGGACAGATTCTCCAATTCAGACTTCTGTGT GCTTCAGGAACCAGAGGTCAGGGTCTGGTCCCATCTCCTGACCAGCAGTGTACTAAGGCTGTTTCCATAT CTCCTACCTTGTCCCCTAGCAGAGATAAGAACACAGGTAGCTCTGAGACTTGCTCTCCCTGGCTGGGGGT AACCTCTGCCCCTGGAGCCTTCCCTTGTTCCTACAATGCCAACCTCTCAGGTTAGACCCAGCCCTGGAGC TCATGGGGTGCCTTGGCCCTGCCCTGCACACCTGCTTCTCTCCATGCATCCAGGTCTATCGACCTGAAGC TGCAGCTGGCCCAAAAAGGACAATAAAACCTGCCACTTTTGCATGAAAAAAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:3584869 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3584869 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7328 same embryo
	EMAGE:7331 same embryo
	EMAGE:7330 same embryo
	EMAGE:7327 same embryo
	EMAGE:7329 same embryo
	EurExpress:euxassay_004412 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source