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EMAGE:7341

Boc biregional cell adhesion molecule-related/down-regulated by oncogenes (Cdon) binding protein ( MGI:2151153)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7341 EMAGE:7341 EMAGE:7341 EMAGE:7341 EMAGE:7341
"Pseudo-wholemount" of euxassay_005272. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005272_01 euxassay_005272_02 euxassay_005272_03 euxassay_005272_04
EMAGE:7341 EMAGE:7341 EMAGE:7341 EMAGE:7341 EMAGE:7341
euxassay_005272_05 euxassay_005272_06 euxassay_005272_07 euxassay_005272_08 euxassay_005272_09
EMAGE:7341 EMAGE:7341 EMAGE:7341 EMAGE:7341 EMAGE:7341
euxassay_005272_10 euxassay_005272_11 euxassay_005272_12 euxassay_005272_13 euxassay_005272_14
EMAGE:7341 EMAGE:7341 EMAGE:7341 EMAGE:7341 EMAGE:7341
euxassay_005272_15 euxassay_005272_16 euxassay_005272_17 euxassay_005272_18 euxassay_005272_19
EMAGE:7341 EMAGE:7341 EMAGE:7341 EMAGE:7341 EMAGE:7341
euxassay_005272_20 euxassay_005272_21 euxassay_005272_22 euxassay_005272_23 euxassay_005272_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7341Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7341_wholemount_strong.wlz
7341_wholemount_moderate.wlz
7341_wholemount_weak.wlz
7341_wholemount_possible.wlz
7341_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7341_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
hypothalamus ventricular layer
moderate moderate
regionalmoderate expression: see section 12 13 14
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 12 13 14
telencephalon mantle layer
strong strong
regionalstrong expression: see section 22 moderate expression: see section 04 05 23 24
olfactory cortex ventricular layer
strong strong
regionalstrong expression: see section 10 11 15 16 17
telencephalon ventricular layer
strong strong
regionalstrong expression: see section 09 10 11 15 16 17 18 19 20 21 22 moderate expression: see section 04 05 06 07 08 12 14 23 24
medulla oblongata basal plate mantle layer
strong strong
single cellstrong expression: see section 10 11 16 17
medulla oblongata basal plate ventricular layer
strong strong
single cellstrong expression: see section 11 12 15 16 weak expression: see section 13 14
rest of cerebellum marginal layer
strong strong
gradedstrong expression: see section 18 19 20 21 22
rest of cerebellum ventricular layer
strong strong
gradedstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 moderate expression: see section 05
pons mantle layer
strong strong
single cellstrong expression: see section 07 08 09 18 19 20 21
pons ventricular layer
strong strong
gradedstrong expression: see section 07 08 09 10 15 16 17 18 19 20
midbrain ventricular layer
strong strong
gradedstrong expression: see section 09 10 11 12 13 14 15 16 17 18
intermediate grey horn
strong strong
single cellstrong expression: see section 08 09 10 11 12 13 14 15
trachea
moderate moderate
regionalmoderate expression: see section 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8930
Entity Detected:Boc, biregional cell adhesion molecule-related/down-regulated by oncogenes (Cdon) binding protein ( MGI:2151153)
Sequence:sense strand is shown

>T8930
AGTTCAGTCATCCAAAAAAGCGTCTTCCATATGGAGGGATTGGGTGCGCTGGGCGCGGCGTCCTCTGCCT
TGCCCGCCTGCTGCCGCCGCTGCCACCACGGCTCTGGCTTCCTGCAGTGGGGACCCTGAGCTGGGTCTCG
CTGGTGTCAGCTCCGGCCGGCCGCATTCTCACACTCTCGCACCTCGTACCCGCCGCGGCCACACAAGCCC
TTCCTCCTCCGGCCGCAACTTCTGGGTCCTGGGCTCTTTTGTTGCTCCGGCTGCGGCCACTCTGCCGTGG
TGTGTGTCCTCTTGTGTCACCGCGCTGTGCGTGTGTGTGTCGGTGTGCGCGGGGGCCGTGTGGCCCTTCT
CCGCTTCACCACGCGGGACCCGGAGTCCCGGCCCCCTCTTCCCAGGCTCCGGCTGCAGGCGAGGATTTCA
GGGGACGGCGGTATCCCTACTTGTATCACACGTGGCTTATGGGACTTTGGCTCAGAACCCTCTGTGACAC
ACTGTACCTCGTGGGAAGATGACGACGTGCCGAAGAGAGCGGCCTATACTTACACTGCTTTGGATTCTCA
TGGCCACAGCAGGCTGCCTTGCTGATTTGAATGAGGTTCCTCAGGTCACAGTCCAGCCCATGTCCACTGT
CCAGAAGCTGGGAGGAACTGTGATCCTGGGCTGTGTGGTGGAGCCACCATGGATGAACGTGACTTGGCGC
TTCAACGGAAAGGAGCTAAATGGCTCTGATGATGCTCTGGGTGTCTTCATCACCCGTGGGACCCTTGTCA
TTGCTGCCCTCAACAACCACACTGTGGGACGGTACCAGTGTGTGGCACGGATGCCTGCAGGAGCTGTGGC
CAGTGTGCCAGCCACAGTGACGCTAGCCAATCTCCAGGACTTTAAATTAGATGTGCAGCATGTGATTGAA
GTAGACGAGGGGAACACAGCCGTCATTGCCTGCCACCTGCCTGAGAGCCACCCAAAAGCCCAGGTCCGGT
ACAGTGTCAAACAGGAGTGGCTGGAGGCCTCTAGAGACAACTACCTGATCATGCCATCCGGGAATCTCCA
AATTGTCAATGCCAGCCAAGAGGACGAAGGCATGTACAAGTGTGCCGCCTACAACCCGGTGACCCAGGAA
GTGAAAACCTCCGGCTCCGGCGACAGGCTGCGCGTGCGCCGGTCCACTGCTGAGGCTGCCCGCATCATCT
ACCCACTGGAAGCCCAGACCGTCATTGTCACCAAAGGCCAGAGTCTCATACTGGAGTGTGTGGCCAGTGG
AATCCCACCACCTCGAGTCACATGGGCCAAGGATGGGTCCAGCATTGCTGCCTATAACAAGACTCGCTTC
CTGCTGAGTAATTTGCTTATTGACACCACCAGCGAGGAGGACTCAGGCACCTACCGATGTATGGCCAGCA
ATGGGGTTGGGGATCCTGTGGCAGCAGTCATCCTCTACAATGTCCAGGTGTTCGAACCCCCTGAGGTCAC
GGTGGAGCTGTCCCAGCTGGTCATCCCATGGGGCCAGAGTGCAAAGCTCACCTGTGAGGTTCGAGGAAAC
CCCCCACCCTCTGTACTATGGCTGAGGAATGCAGTGCCCCTCACCTCCAGCCAGCGCCTCCGGCTGTCAC
GTAGAGCCCTGCGGGTGGTCAGTGTTGGGCCAGAGGACGAAGGCGTGTACCAGTGCATGGCTGAGAATGC
GGTTGGCAGTGCCCACGCTGTGGTCCAACTGAGGACCGCCCGGCCAGACACAACCCTGAGACCCGGGAGG
GATACCAAGCCGATTGCTGCCACACCCCCCATGCCACCCTCCAGACCCAGCAGACCTGACCAGATGCTTC
GGGAACAACCGGGGCTTGTTAAGCCCCCAACGTCGTCGGTACAGCCTACTTCCCTGAAGTGCCCGGGAGA
AGAGCAGGTAGCCCCTGCAGAGGCACCTATCATCCTCAGCTCACCCCGGACCTCCAAGACGGACTCCTAT
GAGCTGGTGTGGCGGCCTCGCCATGAGGGGAGCAGCCGGACACCCATCCTGTACTACGTAGTGAAGCATC
GTAAGGTCACGAACTCCTCTGACGACTGGACCATTTCTGGCATTCCAGCCAACCAGCACCGCCTCACCCT
GACCAGGCTTGACCCTGGAAGCTTGTACGAAGTGGAGATGGCAGCCTACAACTGTGCTGGCGAGGGCCAG
ACAGCTATGGTCACCTTCCGAACAGGACGGCGGCCCAAACCTGAGATCGTGGCCAGTAAGGAGCAGCAGA
TCCAGAGAGATGACCCTGGTGCCAGTCTCCAGAGCAGCAGCCAGCCTGACCATGGCCGCCTCTCCCCCCC
AGAAGCTCCAGACAGACCCACCATCTCCACAGCTTCTGAGACCTCCGTGTACGTAACCTGGATTCCCCGA
GGGAACGGCGGCTTCCCGATTCAGTCTTTCCGTGTAGAGTACAAGAAGCTAAAAAAAGTGGGAGATTGGA
TACTGGCTACCAGTGCCATACCTCCCTCGAGGCTCTCTGTGGAGATCACAGGCCTAGAGAAAGGTATTTC
TTACAAGTTCCGAGTTCGTGCTTTGAACATGTTAGGGGAGAGTGAGCCCAGTGCTCCCTCCCGGCCCTAC
GTGGTGTCAGGCTACAGTGGCCGTGTATATGAGAGGCCCGTGGCAGGACCTTACATCACCTTCACTGATG
CAGTCAATGAGACCACTATTATGCTCAAGTGGATGTATATCCCAGCCAGTAACAACAACACCCCAATCCA
TGGCTTCTATATCTACTACCGACCCACAGACAGTGACAATGACAGTGACTACAAGAAGGACATGGTAGAA
GGGGACAGGTACTGGCACTCCATCAGCCACCTGCAGCCAGAGACTTCCTATGACATTAAAATGCAGTGCT
TCAATGAAGGAGGGGAGAGCGAGTTCAGCAATGTCATGATCTGCGAGACCAAAGCTCGGAAATTTTCTGG
TCAGCCTGGAAGACCCCCACCCTTGACTCTAGCTCCACCACAGCCTCCGCCCCTAGAAACCATGGAACGG
CCGGTGGGCACTGGAGCCATGGTGGCACGGGCCAGCGACCTGCCCTATCTGATTGTCGGGGTTGTTCTGG
GCTCTATAGTCCTCATCATCGTCACCTTCATCCCCTTCTGCCTATGGAGGGCCTGGTCTAAGCAGAAACA
CACAACAGATCTGGGTTTTCCTCGGAGTGCCCTCCTGTCTTCTTCGTGCCAGTACACAATGGTGCCATTG
GAGGGACTTCCAGGTCACCAAGCCAACGGGCAGCCCTACCTTGGTGGCGTCAGTGGACGGGCCTGTGTCA
GTCGAGTGCACGGAAGCAGGGGCTGCCCTGCTGCTACAGTGGGCTGTCCAGGCAGGAAGCCTCAGCAGCA
CTGCCCAGGGGAGCTTGCCCAGCGGGAAGACACCAACAGCCAACTGAGGCAGCCCATTGTTAGCAACGGA
TATGACCTCCAGAACCAGCAGGTTGCCAGAGGTCCCCAGTGTGCCTCAGGAGTAGGAGCTTTCTTATACA
CGCTGCCTGATGACTCAACTCACCAGCTGCTCCAACCTCAAGACTGCTGTCACCTCCAGAAGCAACCCGT
CACCACATGCCAAACAGCAGTGAGGCGAACGTCTGAAAGTCCTGGACTAGAATCTTCATGGGACCCTCCA
TATCATTCAGGGCCCCAGTGCTGTTTAGGTCTTGTACCAGTTGAAGAAGTAGACAGTTCTGACTCCTGCC
AAGTGGGTGGAGGAGACTGGAGTTCCCAGCATCCATCAGGGACCTACACAGGACAGGAACGTGGGATGCG
GTTCTCGCCTAGCCCATCAGTTCATGTGTCCTTTGAAACACCACCTCCCACAATTTAGGCCGAAGCTAAT
ATCCCAGAAAGACTATATATTGTTTGTTTGTTTGTTTGTTGACAGAGAAAAATTGGTATTTATTTTTCTA
TTATAGCCATATTTATATATTTATGCACTTGTAAATAAATGTATATGTGTTTCTATAATTCTGGAGAGAT
ATGGAATCCTCCCCTTTCTGGCACAAGACAGAAAGCAAGCAGCTGAGTAACTGGAGACCAGTGGAAATGA
GTATCATAGATGGTCAGGCTGCAGATGGATCCCAGAACCTGCAGCTACCACGGACACCAGGAGGCCCTCG
GGCCACTTGGTGGAAGGACCCTAAGCACACTGTTCATGAAGAACACAGGGATAAAGCAAAGGCCATCATG
CCGCAGACTGGAGCCACCTGGAAGCCACTCCAATTTGGTGGCACAGGACAGGTTTCCAAGATGTCACGAG
AAAAGCCCCAGATGTGTACAGCACTCTAAGCATTAACAGTCTCCCAGAATCAATAATCGGTGGCAACATA
TCTCTGTAAAAACAAACACTGTAACTTCTAAATAAATGTTTAGTCTTCCTTGTAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6418202 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6418202 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7340 same embryo
 EMAGE:7342 same embryo
 EMAGE:7343 same embryo
 EMAGE:7339 same embryo
 EMAGE:7338 same embryo
 EurExpress:euxassay_005272 same experiment
 MGI:4823492 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS