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EMAGE:7343

Golga1 golgi autoantigen, golgin subfamily a, 1 ( MGI:1924149)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7343 EMAGE:7343 EMAGE:7343 EMAGE:7343 EMAGE:7343
"Pseudo-wholemount" of euxassay_005280. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005280_01 euxassay_005280_02 euxassay_005280_03 euxassay_005280_04
EMAGE:7343 EMAGE:7343 EMAGE:7343 EMAGE:7343 EMAGE:7343
euxassay_005280_05 euxassay_005280_06 euxassay_005280_07 euxassay_005280_08 euxassay_005280_09
EMAGE:7343 EMAGE:7343 EMAGE:7343 EMAGE:7343 EMAGE:7343
euxassay_005280_10 euxassay_005280_11 euxassay_005280_12 euxassay_005280_13 euxassay_005280_14
EMAGE:7343 EMAGE:7343 EMAGE:7343 EMAGE:7343 EMAGE:7343
euxassay_005280_15 euxassay_005280_16 euxassay_005280_17 euxassay_005280_18 euxassay_005280_19
EMAGE:7343 EMAGE:7343 EMAGE:7343 EMAGE:7343 EMAGE:7343
euxassay_005280_20 euxassay_005280_21 euxassay_005280_22 euxassay_005280_23 euxassay_005280_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7343Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7343_wholemount_strong.wlz
7343_wholemount_moderate.wlz
7343_wholemount_weak.wlz
7343_wholemount_possible.wlz
7343_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7343_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8929
Entity Detected:Golga1, golgi autoantigen, golgin subfamily a, 1 ( MGI:1924149)
Sequence:sense strand is shown

>T8929
CATGGCCTCCGGCGCTCTACCGCGAGGGCAGTGACTTTGACCTCCATACTCTTTTAAATATAGTAGATAA
GACAGATCTTGTGTTGCCAAAATTCTTTGGCCCACATTTAAGTGGGATGCAGCTAGCCTGGAGGTGTGAA
TGAATTTCATTCACTGAGAAGTCTAGGGGTTAGTTAACCCTGAAAGATATCCACACAGCAAAAATGTTTG
CAAAACTAAAGAAGAAAATTGCAGAAGAGACTGCTGTTGCTCAGAGACCAGGCGGCACAACAAGGATCCC
ACGCTCTGTGAGCAAGGAGTCTGTGGCCTCCATGGGAGCTGACTCTGGAGATGATTTTGCTTCCGATGGT
AGCAGCTCCAGAGAAGATCTCTCATCCCAGCTTTTGAGAAGAAATGAACAGATACGGAAGTTAGAGGCCA
GACTTTCTGACTATGCTGAACAGGTCCGAAACTTGCAGAAGATAAAAGAGAAGCTTGAAATTGCATTAGA
AAAACACCAGGATTCTTCCATGCGGAAATTTCAAGAACAGAATGAAACATTCCAAGCCAGCAGAGCCAAA
ATGGCAGAAGGACTGGCTTTGGCTTTAGCCAGGAAGGACCAGGAATGGTCAGAAAAGATGGAACAGCTTG
AAAAGGATAAAAGGTTTCTAACATCTCAGCTGCAAGAAGTAAAGAACCAGAGCTTGAGCCTTTTTCAAAA
AAGAGATGAAATTGATGAATTAGAGGGGTTCCAGCAGCAGGAAATCAGCAAAGTAAAACACATGCTTTTA
AAAAAAGAAGAATGCTTGGGGAAGATGGAGCAAGAATTGGATGCACGAACCAGGGAACTTAACCGTACTC
AGGAGGAGTTGGTGACTTCTAATCAGTTGTCATCTGACTTAAACGAGCGGCTAGAAGAATTGCAGAGACA
CTGCTCAACGCTGGAAGAGCAGAGAGATCACTTGACAGCTTCAAAAGCAGGTGCAGAACATAAGATTGTA
GTCCTGGAACAAAAGGAACAAGAGCTCCAGGCGATCATCCAGCAGCATTCCATTGACCTACAAAAGGTCA
CTGCTGAAACGCAAGAAAAAGAAAAAGTCATCACACATCTACAAGAGAAGGTTATATTCTTGGAAAAGAG
ACTAGAACAGAATTTATCAGGAGAAGATCATGTACAAGAACTCTTGAAAGAGAAAACAGTTGCTGAACAG
AATCTGGAAGATACTAGGCAGCAGCTCTTGGCTGCCAGGAACAGCCACACCAAGGCACTTTACCTCCTGG
AGACTCGGGTGAAGGACCTAGAGCGGAGTCTACAGGCTGCTGAAGAACAGCTAAGCCAGAGTAGAAATGT
TGTGGCTGACCAGGAAGCCCAGATCCAGAAGCTTATCACTACAAACCAGGAGAACAGCCTTTCCCAGCAG
CAGGTTCTTGCCCTGGAACAGCATTGCAGGGAGCGCATCCATGCACTGGAGGCCCAAATTGAAGCTCTGG
AACAAACACGGGTAGCTGACCAGATAGCCTCAGAGCAGGGAATGCTACAACTGCAGCAGGAAAATGTGGC
CCTTAAAGAAAGCAGAAATGAATGTGAACATTCCTTACAACATCACCAATTGGAACTAAAGAAACTAAAG
GATGAATGGAGCCAAAGAGAAATTGTGAGTGTAGCAATGGCTCAAGCCCTGGAGGAGGTGCGGAAACAGA
GAGAAGAGTTCCAGCAACAGGCCACTGAGTTGACAGCCATCATAGAAGAGAAAAATCAGAGCCTGTGTGA
AAAGGATGAGGCACTTCTCCAGAAGGAGCAGGAACTTCGACAGCTGGAAAAAGGTCACAGTTCTGCCCTG
CTGCAGATGCACAAACTGCAGAGGGAACTAGAGGCCTTGAAGACTTGCAAGGCTCAGGAGGCAATGCCAG
CCACAACAGGAGAGGACTGCCTGCCACTGCAGGGCCAGGAGCCACTTGTCATTTCAAAAGCCATGCAGAA
TTCTGAGTATGAGCTTCCAGCTGCAGAAGGAACTCCAAACGGTGAGGTTGGAGCCAGTGATCTAAAGCAG
CTACAGAAAGAAAAGCAGGACTTAGAACAGCAACTTATCGAGAAAAATAAGATTATGAAGCAAATGCAGC
AGAGGATGCTGGAACTTAAGAAGACTCTACAGAAAGAGTTGAAAATCAGACCTGACAGTGAGCTTTTTGA
GGTCCGGGAGAAGACAGGCCCTGAGATACCAAATATGGCCCCTTCTGTCACTAATAATACTGACCTGACT
GATGCCCGAGAGATCAACTTTGAATACCTTAAACATGTTGTTTTAAAATTCATGTCCTGTCGAGAATCTG
AGGCTTTTCATCTCATAAAAGCAGTGTCAGTGCTGCTGAATTTCTCACAAGAAGAGGAGAATATGCTCAA
AGAGACTCTGGAATATAAGATGTCTTGGTTTGGATCCAAACCAACTCCCAAGGGCAGCATCCGGCCATCT
ATCTCCAACCCTCGGATACCATGGTCCTAGAGCCATCCAAGGATAGAGCTACATGGGCTGACACTCTTCC
TGTGAAAAGAATACTGACACACCAGACTGGGTGGGTTTATACATCGATGTAACTACAGTATTTTGTACAA
GTGTCTAGACAGTTTACAAGACTTTAGGCCCACCTCCCTGTAGACTGATAATGAACTTCAAAGGGAGCTA
ATTCTATCATAATGGTTATTAAATGAGAAGGAATCCTGGGCACTGCCCAGATTTCCACAGTGCTGCTAAT
ATCCCAGCTCTGGCCAGCTGCAACTGAGTCCTCTATCTTTTAACATTAGCACTTAAAGATGACGTGATTG
GCATGTCAGGTGAAAATTAATTATGAAGCAGAGGTAACAGGACTGAAGGTGGAGATAGTCCTTTCATAAA
AAGCCAACTCAAGGGTGGTCTTTGGAGTCCCCTTCCACTAGCATGTTTGGGTTTTCATGCACTGTTGCCT
CTCAAGAAAGTATCATTACACTTTGTTTCCCCAGCCACCTAGAACTTGAGATGTGTCCATCTCATAGGTT
ACGGGGCCAGTGTGGACTGATGAGTATGCAACTTTCCAGTCCTGAGAAAACTAGCAAGCAGACGGAATGA
TCTCTCCGTATAGCACCAAATACACTCAATTGCCTTCTTAATGAATGTACTCGTCTTGGATGGGTTGCTG
GTAAACCATTTTAAAACTATTTTTATAGCAAAAGTTCTTCGCTATTCTAAACATGTCTTCTGTATTATAA
AATCCATTTAAAGATGAAAATCAGAGCTTCAAGTCTTTCTAGTGCCAGGACATATGCCCCTTTGTAATTT
TTCTTGCCTTCTGTGGTACCTGCTCTCTAAAGCAGTAGTTTCTCCAGCTTTGCTCTGAATTCTCTCATAT
TACCACTGAGCAAATGCCAGTCACACTTCTACTTAAGAATAACCTGCTGAATTCAATGGAAGCAGGCTGT
CCCTCTCCTACCCTCGTTATTTCTGATGACACTACATTGATATATAGCACTCAGGCAGCCCAGCTGACTT
CCACACTTGATAGCACACATGCCTCCGTTTTAGCTGATAGAAACTTACATTCATTTTTACACTTGATCTG
GATAAGCCACATCAGATCCCAAGGGAAGCCATTTGATTGAGTTTGATGAAAGTGTTTCACTAGCGAGTAA
GCCAATAATTGGAGCTTTTAAAGTTACCATGTATCTAGGTAAATTTATGTTCTTCAGCTTAAAGGCAACA
TATGCATGTACATGCATATAATGTGTCATACAGAAATCGAAGTTTTGTAGCATTTGCCTTCTAAGTCTAA
ATCAGGATGGCATTGCTTTGGCTGTGGCTCTTCTTACAGGAGTTTCCCTTTAGGGTGCCACTTCCCATTT
AGTGGGGACATGAAACTAGAGCCCCACTGTTATGTGATTTTACAGAGGAAGATGTAGCTTCTCCAAGTAG
GATTCCTTCCCTTCCTCCTGTCCCAGGTAAGAAACCCCAGAGGACTGAACAGTCCACAGCCCTCCAGAAG
CAGCTGCAGTTCCAGCTTTGGAAGCCCTTTCTACACCTTAGGTTTCTCAGGCCTTTGAGCTGGTATGTCC
GTCTGTCTATCTGTCTCTCTCACACTCGAGCACACAGACACCCCTGCCTGCAGGTTGTGTTGTGGCTGTG
GGGCCCACTCGTTTAGAATGGCTTTCCTGCTGCATGTTGAGCTCCTCCAGCACTAGGAGAGTGCAGTAGC
TATTCCAAGTGGCTTCATGCACTTTACTTCCATAAACTTTATAAGAGACAGTGTGTAGCCCTTTATAGAT
GAGCACTGGACTGTGATCTTACTACCAATGTAAATAGGGATAGTATTATCTAAAAACTGCTGTAGCAGAA
TTATTTGTGGTGCAATACTTTTTGATTAAAGCTCTTCAAGATGCAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6418353 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6418353 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7340 same embryo
 EMAGE:7341 same embryo
 EMAGE:7342 same embryo
 EMAGE:7339 same embryo
 EMAGE:7338 same embryo
 EurExpress:euxassay_005280 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS