EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7423

Hoxd10 homeobox D10 ( MGI:96202)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004438. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004438_01	euxassay_004438_02	euxassay_004438_03	euxassay_004438_04

euxassay_004438_05	euxassay_004438_06	euxassay_004438_07	euxassay_004438_08	euxassay_004438_09

euxassay_004438_10	euxassay_004438_11	euxassay_004438_12	euxassay_004438_13	euxassay_004438_14

euxassay_004438_15	euxassay_004438_16	euxassay_004438_17	euxassay_004438_18	euxassay_004438_19

euxassay_004438_20	euxassay_004438_21	euxassay_004438_22	euxassay_004438_23	euxassay_004438_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7423_wholemount_strong.wlz

7423_wholemount_moderate.wlz

7423_wholemount_weak.wlz

7423_wholemount_possible.wlz

7423_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7423_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 23 24

hindlimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 18 19 20 21 22 23 24

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14

rectum

moderate

regional

moderate expression: see section 11 12

midgut

moderate

regional

moderate expression: see section 05 06 07 08 09

bladder

moderate

regional

moderate expression: see section 12

metanephros

moderate

regional

moderate expression: see section 06 07 08 09 10 15 16 17 18 19

ureter

moderate

regional

moderate expression: see section 07 08

tail

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7548
Entity Detected:	Hoxd10, homeobox D10 ( MGI:96202)
Sequence:	sense strand is shown >T7548 CGGACGCGTGGGTCGACCCACGCGTCCGCCCACGCGTCCGCCTCCCCAAAATGTCCTTTCCCAACAGCTC TCCTGCTGCTAATACTTTTTTAGTAGATTCCTTGATCAGTGCCTGCAGGAGTGACAGTTTTTATTCGAGC AGCGCCAGCATGTACATGCCACCACCTAGCGCAGACATGGGGACCTATGGAATGCAAACCTGTGGACTGC TCCCGTCTTTGGCCAAAAGAGAAGTGAACCACCAAAATATGGGTATGAATGTACATCCTTATATACCTCA AGTAGACAGTTGGACAGACCCGAACAGATCTTGTCGAATAGAGCAACCTGTTACACAGCAAGTCCCCACT TGCTCATTCACCGCCAACATTAAAGAAGAATCCAATTGCTGCATGTATTCTGATAAGCGCAACAAACTCA TTTCTGCCGAGGTCCCTTCGTACCAGAGGCTGGTCCCCGAGTCTTGTCCTGTTGAGAACCCCGAGGTTCC TGTCCCTGGATATTTTAGACTGAGTCAGACCTACGCCACCGGGAAAACCCAAGAGTACAATAACAGCCCC GAAGGCAGCTCCACTGTCATGCTCCAGCTCAACCCTCGTGGCGCGGCCAAGCCGCAGCTCTCGGCTGCCC AGCTGCAGATGGAAAAGAAGATGAACGAAAGCGCGAGCGGCCAGGAGCCCACTAAAGTCTCCCAAGTGGA GAGCCCCGAGGCCAAAGGCGGCCTTCCAGAAGACAGGAGCTGCCTGGCTGAGGTTTCCGTGTCCAGTCCC GAAGTGCAGGAGAAGGAAAGCAAAGAGGAAATCAAGTCTGATACTCCAACCAGCAATTGGCTCACTGCAA AGAGTGGCAGAAAGAAGAGGTGCCCTTACACCAAGCACCAAACGCTGGAATTGGAAAAAGAGTTCTTGTT CAATATGTACCTCACCCGCGAGCGCCGCCTAGAGATCAGTAAGAGCGTTAACCTCACCGACAGGCAGGTC AAGATTTGGTTTCAAAACCGCCGAATGAAACTCAAGAAGATGAGCCGAGAGAATCGAATCCGAGAACTGA CCGCCAACCTCACCTTTTCTTAGGTCTGAGGCCCATGTGAGGCCAGGATCCGAGAGGGGGCACCGAGTTC CAGGCCTGAGTGCTGGAGGACTGGCAAGGCGGAAACAAACCCTTCATTGCTCTTTGGTTTTTTTGTTTTG TTTTGTTCTGTTCTGTTCTGTTTTCCTGCTAGAATGTGACTTTGGGGTCATTCTGTTCGTGCTGCAAGTG ATCTGTAATCCCTATGAGTATACATATATATATATATTAAAAAAACTTAGCACGTGTAATTTATTTTTTT TTCATCGTAATGCAGGGTAACTATTACTGCGCATTTTCATTTGGGTCTTAACTTATTGGAACTGTAGAAC ATCCATCTAAACGTATCCATCGCATCCCACCCATCTATCCCGCCAGCAATGTGACTTTTCCATGTTTTTC CTAACATAAAAGTTCTCTGTGTGTGGTTAGCCCATGAACTCATGGCATTTTGAAAACATCCAGTATTTAA AAAAACAAACAACCTATATCTATATATATTTCAAAAGATTAAGAAAACCCACAAGCTTTGGGGGGAGGGG GACTTTAAAAAGCACATTACAATGTATCTTTTCACAAATGAACTTAGCAATTGTCCTTGGTGAAATGGAA TATTGATGACTTATGCCTTGTAGCCTTTCCTTGTGGTGCATCTGTGGTTTGGTAGAAGTACAAGCGCAAC CTGTGCTTTTCTGTGCATGTTCTGGTCGCATGTGTAATGCAATAAACTCTGGAAATGGGAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4240579 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4240579 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7422 same embryo
	EMAGE:7420 same embryo
	EMAGE:7419 same embryo
	EMAGE:7418 same embryo
	EMAGE:7421 same embryo
	EurExpress:euxassay_004438 same experiment
	MGI:4825433 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source