EMAGE mouse gene expression spatial database

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EMAGE:7441

Lpp LIM domain containing preferred translocation partner in lipoma ( MGI:2441849)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004593. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004593_01	euxassay_004593_02	euxassay_004593_03	euxassay_004593_04

euxassay_004593_05	euxassay_004593_06	euxassay_004593_08	euxassay_004593_09	euxassay_004593_10

euxassay_004593_11	euxassay_004593_12	euxassay_004593_13	euxassay_004593_14	euxassay_004593_15

euxassay_004593_16	euxassay_004593_17	euxassay_004593_18	euxassay_004593_19	euxassay_004593_20

euxassay_004593_21	euxassay_004593_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7441_wholemount_strong.wlz

7441_wholemount_moderate.wlz

7441_wholemount_weak.wlz

7441_wholemount_possible.wlz

7441_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7441_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7653
Entity Detected:	Lpp, LIM domain containing preferred translocation partner in lipoma ( MGI:2441849)
Sequence:	sense strand is shown >T7653 AAGAGGTAATCCGCCACATCCCTGCTGTGTTCTGTCCTGGGCCTGGCTTGGTGAGAGGGAGTCAGGGCAG TGAAGGCCAGCTCTCTGTGGCGGTGACCTCCTCAGTGGGAACACAGGCTCAAGGACAGAAGGAGAGAGAG CGAGCCAGGTGCTTCTGCTTTTGTTACTTTTTCACCGTTCCCCCCACTGGAGGGAAGACATTGTTCCTCA GTTGAGCTGAGCACATCGTCTGGCTAAGCCTGTGACCATTGTCCATTCCAAAAGCCAAAGGCTGCTGGAG ATTTCCACAATGTCTCACCCATCTTGGCTGCCACCCAAAAGCACTGGTGAGCCTCTTGGCCACGTGCCTG CACGGATGGAGACCACCCATTCTTTTGGGAACCCCAGTATCTCAGTGTCAACACAACAGCCACCCAAGAA GTATGCTCCAGTTGTTGCTCCGAAACCCAAGTATAACCCATACAAGCAGCCTGGAGGAGAGGGTGATCTT CTCCCACCGCCACCACCACCTCTAGAGGATCCTGGTACCATCCCATCTGGTCCCGGACACTTCCCTCCTC CTCCACCACTGGATGAAGGGGCTTTCAAAGTGCAGCAGGGAAATCCTGGAGGCAAGACCCTGGAGGAAAG GCGCTCTAGTCTAGATGCAGAGATCGACTCCTTGACCAGCATCTTGGCTGATTTGGAGTGCAGCTCACCC TACAAGCCACGTCCCCCACAGGGCTCTGCTAGTTCAATAGCCTCTCCTCCAGTTTCTACTCCTGTCACAG GACATAAGAGAATGGTCATTCCACAGCAGCCACCCCTGACAGCAACCAAGAAGTCCGCAACAAAACCACA GCCTGCACCCCAGGCTGCACCCATCCCAGTGACTCCAATTGGAACACTCAAACCCCAGCCTCAGCCAGTT CCAGCCTCCTATACTACGGCATCCACATCTTCAAGGCCCACCTTTAATGTGCAAGTGAAGTCAGCTCAGC CCAGCCCACATTACATGGCTGGTCCATCCTCTGGACAAATATATGGTCCAGGGCCTCGTGGCTATAACAA CCAGCCAGTTCCTGTATCGGGACAGTGTCCTCCTCCTCCAACCTGTGTTGGTACCGACTATGCATATATT CCACCATCTGGACATCCGCCTGAGTCTGGATATGGGTATACCTCCAACCAAGGACGCTATTATGAACCTT ACTATGCAGCAGGTCCTAGCTATGGGGGCCGAAGTGAAGGTGATACTGCTTATGGGCAACAGGTTCAACC TAATACCTGGAAGCGGGAAGCTGCGTATGCCCCTCCTGCAAGCGGGAATCAGAACCATCCTGGCATGTAT CCAGTCAGTGGCCCCAAGAAGACCTATATCACAGACCCTGTTTCAGCGCCCTGTGCACCACCACTGCAGC CAAAGGGTGGATACCCTGGGCCTATGGGGCCTCCTTCCATCCCCCCATCATTCCGCCCAGAAGATGAGCT CGAACACTTGACCAAGAAGATGCTGTATGACATGGAGAACCCACCCGCTGACGATTACTTTGGCCGTTGT GCCCGCTGTGGGGAAAATGTTGTTGGAGAAGGAACAGGATGTACTGCCATGGATCAGGTCTTCCACGTGG ATTGCTTCACCTGCATTGTCTGTGATGTCAAGCTCCGAGGGCAGCCTTTCTATGCCGTGGAGAAGAAGGC ATACTGCGAGCCCTGCTACATTAATACCCTGGAGCAGTGCAGTGTGTGTTCGAAGCCCATCATGGAGCGG ATCCTCCGTGCCACCGGGAAGGCCTACCACCCCCACTGCTTCACCTGCGTGATGTGCCACCGTAGCCTGG ACGGCATCCCATTTACTGTGGATGCTTGCGGTCTCATTCACTGCATTGAGGACTTCCACAAGAAATTTGC CCCGCGCTGTTCTGTGTGCAAGGAACCTATCATGCCAGCCCCAGGCCAGGAGGAGACTGTCCGCATTGTG GCTCTGGACCGTGATTTCCATGTGCACCGCTATCGCTGTGAGGACTGTGGTGGTCTTCTGTCCGAAGGAG ACAACCAAGGCTGCTACCCCTTGGATGGACACATTCTCTGTAAGACCTGCAACTCGGCTCGCATCAGGGT CTTGACGGCCAAGGCAAGCACTGACCTGTAGAGTCAATAGCCAGCTCAGCTGCCGAGTGCATGCCAGCAA GAAGAGCGCAACACAAGAAAAAGATAAGAGAAGAAGTAGAAAAATAGAGGCATAGCTCAACCACTAGGAT AGTCTCTGTTCTTCATGTGCTATTAACCTTTCTGTAGAAGCGCATAGAGAATACGATTTGTCTACGTTCT TACCAAATACACATTTCACATTTAATCATGCAAGGCCTTGGTGAGCCTTTGTTCCAAGGACTTCCACATT TTTGCACAGATTATACTCCGTCCCATTCACTTCTGCATTCCTGAGACTTTTAATCCTCATGTTTGTCTCA CTTCTCATGTGGTTGAATAGATTTTTAGTGTGGTATTTGCTGTCACATGATTTTTCCTGGGTGAATGTGC CAGCCTGCAGGTGCCTTAAGTCTTGTCCTGTCTGTCACTTTTCCTCTGGCTGTGGCTACAAAAGGCAGTC ATTCTGTTACCAGGTATTGAAAAGGTGAAGTTTCCCATTGCACTACACCCCTGACGTCTCTTGCTAAAAT AATGGTAGGGGGATATTTTTCTATGTTAAGCTATAAAGAGTTTATTTGAAAAAGTTAACAGGAGCCCACT TTAGTCTGCAACTATCCAAATTCAGCAGTCAAGGCTCATTGGAAAACATTGATTAGCCAATATCTAAAAT GTGGGGAAATAAAACCAAGTTTCACCACTGTGCTAAAGAGGCAGAATTCTAGGTGTATCTGGAAGGCCCG TTGTATTTCCACAATCCCACCCTGAGAGTTAGCTCACCACACTCTCTGGATATAAGACTGTCAGGCTTGC TTTTGGTACATTTATAAATCTATCCAACTCTTCATCCTTAAAGCACTGTGAAACTTCACCTAATATATAA GCATGGTTACTGCAATTAATATTTATCTTCAGAAAGATAAGTATTAAAATGAATCAAATTCTTATCTTTC TTTCTTTCACTTTATAGTGACCCAAACCTAAATCTCTTTTGCCTGAAAATTGCAAATCGGGACACATAAA ATGAAATTATAGGACCATGTTTCATCTTCTTTAAAAAAGAGAAAAAAAAGCTGGGAAAGATTGTGAAACC AGAGTTTATTATTCTTACAATCCTCTGTAACTCAAGTACATACTGCAGGAGACATTTTCCTATCATCAAC GTGACATTCACACCACACTTTCAAAGACAATCGCTGAAAACCAAATTGTCTTTCTGAGGTAAAAATATGC GGAATGCCTGCCTGGAATCTTTATGCGAACTCTTATTAGCCAGTGAAACACTTGCTTGCCAACTGGAGCC AGTTCGCTTCGTTAAGAGGAGACACCTCGCTTTGCAGTCGCAGTTGGCCATTATTGTAAAGTCAGTATCT CAGAATGAGATTTCTCAAAGCTTTCTTTAAATCTTCTACAGATATATACCTTTAAATTATGGAATACCTT AAATATTACAGAAAGGTATAAATAATAATATAAAGGTCCCTCTATAGCTAACACCGTTCTAGAAGTATAG TCACTTTAAATTTTTTAAACTCCACAAATATCTTTTTATCTCTTTTATATAGTACTGGGAGTTGAACTAG GGCCTCATGTGCTCGGCATGTGCCCTGGCACCCCAGCATCCCCAGCTCTTCCGTGTCTTTTTCTTTTTTT TTTAATCTTTATGACATGATGATTAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30743832 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30743832 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7442 same embryo
	EMAGE:7440 same embryo
	EMAGE:7439 same embryo
	EMAGE:7438 same embryo
	EMAGE:7437 same embryo
	EurExpress:euxassay_004593 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source