EMAGE mouse gene expression spatial database

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EMAGE:7486

Mapk11 mitogen-activated protein kinase 11 ( MGI:1338024)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004625. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004625_01	euxassay_004625_02	euxassay_004625_03	euxassay_004625_04

euxassay_004625_05	euxassay_004625_06	euxassay_004625_07	euxassay_004625_08	euxassay_004625_09

euxassay_004625_10	euxassay_004625_11	euxassay_004625_12	euxassay_004625_13	euxassay_004625_14

euxassay_004625_15	euxassay_004625_16	euxassay_004625_17	euxassay_004625_18	euxassay_004625_19

euxassay_004625_20	euxassay_004625_21	euxassay_004625_22	euxassay_004625_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7486_wholemount_strong.wlz

7486_wholemount_moderate.wlz

7486_wholemount_weak.wlz

7486_wholemount_possible.wlz

7486_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7486_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 17 18 19 20 21 22 weak expression: see section 08

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 17 18

trigeminal v nerve

moderate

regional

moderate expression: see section 09 16

spinal cord

weak

homogeneous

weak expression: see section 09 10 11 12 13 14 15 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 15

cervical ganglion

moderate

regional

moderate expression: see section 09 16

dorsal root ganglion

moderate

regional

moderate expression: see section 15 16 17 18 weak expression: see section 07 08 09 14

not examined

regional

not examined expression: see section 01 02 03 22 23

neural retina

moderate

regional

moderate expression: see section 01 02 03 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 16 17 weak expression: see section 10 11 14 15 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7615
Entity Detected:	Mapk11, mitogen-activated protein kinase 11 ( MGI:1338024)
Sequence:	sense strand is shown >T7615 GCGCGCGGGGATCCGGGGCGCTCGCTCCAGCTGCTTCTGTGGATATGTCGGGTCCGCGCGCGGGATTCTA CCGGCAAGAGCTGAACAAAACAGTATGGGAGGTGCCGCAGCGGCTGCAGGGCCTACGCCCGGTGGGCTCC GGCGCCTACGGCTCAGTCTGCTCGGCCTACGACGCGCGGCTGCGCCAGAAGGTGGCTGTAAAGAAGCTGT CTCGCCCTTTCCAATCGCTGATCCACGCGAGGAGGACATACCGTGAGCTGCGCCTACTCAAGCACCTGAA GCACGAGAACGTCATAGGACTTTTGGACGTCTTCACGCCGGCCACATCCATCGAGGATTTCAGCGAAGTG TACCTCGTGACGACCCTGATGGGCGCCGACCTGAATAACATCGTCAAGTGTCAGGCCCTGAGCGATGAGC ATGTTCAATTCCTTGTCTACCAGCTGCTGCGTGGGCTGAAGTATATCCACTCGGCGGGCATCATCCACCG GGACCTGAAGCCCAGCAATGTAGCGGTGAACGAGGACTGCGAGCTGAGGATCCTGGACTTTGGGCTAGCA CGCCAGGCTGATGAGGAGATGACCGGATATGTGGCCACACGGTGGTACCGGGCGCCAGAGGTCATGCTAA ACTGGATGCACTACAACCAGACAGTGGACATCTGGTCTGTGGGCTGCATCATGGCTGAACTGCTGCAAGG AAAGGCCCTCTTTCCTGGAAACGACTACATCGACCAGCTGAAGCGAATCATGGAGGTGGTGGGCACGCCC AGTCCTGAAGTTCTGGCAAAGATATCCTCGGAGCATGCCCGGACATACATCCAGTCTCTGCCTCCCATGC CCCAGAAGGACCTCAGCAGTGTCTTCCATGGAGCCAACCCCCTGGCCATAGACCTCTTTGGAAGAATGCT GGTACTAGACAGCGACCAGAGGGTCAGTGCGGCCGAAGCCTTGGCCCACGCATACTTCAGCCAGTACCAT GACCCTGACGATGAGCCAGAGGCAGAGCCCTATGATGAAAGTGTTGAGGCCAAGGAGCGCACGCTGGAGG AGTGGAAGGAGCTTACTTACCAAGAAGTCCTTAGCTTCAAGCCCCTGGAACCCTCACAGCTCCCTGGCAC CCATGAAATTGAGCAGTGAGGCATTGCCTGAAGTGGGAGACCTGAGCCTGTCCCCTCTACTCAGCTTTCC GGCTTTCCTCAAGAGGCGCCTCTCAGGCAGCCCTGACACTTAACCTGGGACCGACCCCCTTGCCTTGAGA AACTCTACACACACACACACACACACACACACACACACACACACAGAGGAATGCACGGATGTGTACATAT GCCTCCCTCCCATTATGTGCTGGAGTCTGGGCAGTAGTGTTTCTGGACCTACCTTGGTCCACCCAGGTTC TCCTTGGGACCTCCGTGTGTGGGATCTGAGTGTGTCCCTGTCCTTGTCCAGGTCTCTTCTGCTCTTGAGG ACTATCTGGATTGGGAGGAAGGCTAATGGGCCATGGTCCCTGGAGACTCTGAGGGAGGGGGAGTCACAGT GGTTGGAGATGCTCAACCAGGAAGTGAGTGGCTGAAAGCAGAGGCAAGCCCAGTGTCCCTCCTAACTGAG TGGCGTTTCCTCTGGGGATGGGAAAGCTGAGACCACCATCAGATTATCTATCTAGTCAGAAAAGCCTGCT GAAGATTTGTGGTTGCCTGTGGTATGTGGGCACAGGGGCATGAGTATGCACCCTGGGATACACATCAGAA CAAATGCAGGCATGTGCTGGTGTGTATGAATCTCTGAGCACCCAAGAGGCAGCAGCCATTTCTGGTCTGG TGCCTAAGTTGGAGATAACTCTTGCCTCCTCTCCCCCCAAGTTCCTGTTTTCTAATGGGTCTTGAATCCC AGCTGTGAACTAGACCAAAGGCTCTGAGAGGTGCCAAGATCCCTTAGTTTAAGCAAATGTAAGGGTATAG GAGCAGCAGCAGGATCCTGAGTCACAAGGGCACAATGCCAGCTGGAGAGCTGATGAAGTTGGGGGTCTAA TCTCAAGGATGCTAGGTGGCCAGCACCCACGGATGTGAGCATGGACATTCTTGCCCCGGGACCTTGATTC AAAGTTGACAAACGTCTGTCCACGTTCTACCTCCACCAACCCTTGAGGTTCTGCAGCTGGTCTGGGGTGC TATGGTTTGCAAGTCACCCCTCATCTCCCCATCCCCCAGAGAGGAGCTGACCAAGTAACTTTTGAGGTAG GGCCCTGTTTGAAAACGCGTTGGGGGTGTAACGCCTCCCTGAAGAGACAATGATCTCTTGCTTATCAGGG ACCAAGGGGCTCTGGATGTCAAGTGCCTGCACCAAGAGTGTGCACAATAAAGGGGCATCCCTCTTGCTCT TGTCTGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30112690 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30112690 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7482 same embryo
	EMAGE:7487 same embryo
	EMAGE:7485 same embryo
	EMAGE:7484 same embryo
	EMAGE:7483 same embryo
	EurExpress:euxassay_004625 same experiment
	MGI:4826087 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source