EMAGE mouse gene expression spatial database

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EMAGE:7488

Entpd5 ectonucleoside triphosphate diphosphohydrolase 5 ( MGI:1321385)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005218. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005218_01	euxassay_005218_02	euxassay_005218_03	euxassay_005218_04

euxassay_005218_05	euxassay_005218_06	euxassay_005218_07	euxassay_005218_08	euxassay_005218_09

euxassay_005218_10	euxassay_005218_11	euxassay_005218_12	euxassay_005218_13	euxassay_005218_14

euxassay_005218_15	euxassay_005218_16	euxassay_005218_17	euxassay_005218_18	euxassay_005218_19

euxassay_005218_20	euxassay_005218_21	euxassay_005218_22	euxassay_005218_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7488_wholemount_strong.wlz

7488_wholemount_moderate.wlz

7488_wholemount_weak.wlz

7488_wholemount_possible.wlz

7488_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7488_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

naris

strong

regional

strong expression: see section 10 13

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 13 14 16 17 moderate expression: see section 07 08 11 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7591
Entity Detected:	Entpd5, ectonucleoside triphosphate diphosphohydrolase 5 ( MGI:1321385)
Sequence:	sense strand is shown >T7591 GGCAGGCAGGAGCTGCTTTTTAAATTGGCCTGCGTGACCCGCCCACTTGGTGTAAAAGAAGAACCGGCCA AAGGGAGGGCCTGAAGGACCTCCACAGGAGTGTGAGCAGCACTGCTTCAGCAACAAAGCCTCAGGTCCAC ATCTTGGGAAGAATATGGCCACTTCCTGGGGGGCTGTCTTCATGCTGATCATAGCCTGCGTTGGCAGCAC TGTCTTCTACAGAGAACAGCAGACCTGGTTTGAAGGTGTCTTCTTGTCTTCCATGTGCCCCATTAATGTC AGTGCCGGCACCTTTTATGGAATTATGTTTGATGCGGGCAGCACTGGAACTCGGATTCATGTTTACACTT TTGTGCAGAAAACAGCAGGACAGCTCCCCTTTCTGGAAGGTGAAATTTTTGATTCTGTGAAGCCGGGACT TTCTGCTTTTGTGGATCAGCCCAAACAGGGTGCTGAGACTGTCCAGGAGCTCTTGGAGGTGGCCAAAGAC TCGATCCCCAGAAGCCACTGGGAAAGGACCCCGGTGGTTCTGAAAGCAACGGCCGGACTCCGTTTGCTGC CTGAGCAGAAAGCCCAGGCTCTGCTCTTGGAGGTAGAGGAGATCTTCAAGAATTCACCTTTCCTGGTCCC AGATGGCAGCGTTAGCATCATGGATGGGTCCTATGAAGGCATACTAGCCTGGGTTACCGTGAACTTTCTA ACAGGTCAGCTGCATGGTCGTGGCCAGGAGACTGTGGGGACCCTTGACCTGGGGGGTGCCTCCACCCAAA TCACGTTTCTACCCCAGTTTGAGAAAACCCTGGAACAAACACCTAGGGGCTACCTCACTTCCTTTGAGAT GTTTAACAGCACTTTTAAGCTCTATACACATAGTTACTTGGGATTTGGACTGAAAGCTGCAAGACTGGCA ACTCTGGGAGCCCTGGAAGCAAAAGGGACTGATGGACATACGTTTCGAAGTGCCTGTTTACCAAGATGGT TGGAAGCAGAGTGGATCTTTGGGGGTGTGAAATACCAGTATGGTGGTAACCAAGAAGGGGAGATGGGCTT TGAACCCTGCTATGCGGAAGTGCTGAGGGTAGTACAGGGGAAACTTCACCAGCCAGAAGAAGTCCGAGGA AGCGCCTTCTACGCTTTCTCTTACTACTACGATCGAGCCGCTGACACACACTTGATCGATTATGAAAAGG GCGGGGTTTTAAAAGTTGAAGATTTTGAAAGAAAAGCCAGAGAAGTGTGTGACAACTTGGGGAGCTTCTC CTCGGGCAGTCCTTTCCTCTGCATGGACCTCACTTACATCACAGCCCTGTTGAAAGATGGTTTTGGCTTT GCCGACGGCACCCTCTTACAGCTCACAAAGAAAGTGAACAACATAGAGACTGGTTGGGCCTTGGGGGCCA CCTTTCACCTGCTCCAGTCTCTGGGCATCACCAGCTGAGGCCAAGCTCCACCTCTGAAGCCTGCATTTCT GAACAGTTTTCTCACAGGAAGGCGTGGACTCAGAGACATTTTCTGACCTCTCTGGAAAAGCCTGGCCCGA AACCCGTTAACTGGTTTTATAAGGAGGGAGGGGTTTTTAGATGAGTCTTGCTCTTGAGCCTAGTGATTTG GGCTTCAATGATTTGCACATCTAATGTGAATAGCTCCTAACCACTTGGTGGGTGCATGGCTGGCACCAGA CTGTAAATCTTTTGGGATTCTTTGTTACAGAGTCCTGCAAAGGAAAAAAGAGAAAAGGTTTGGAACTCCA TGCTAGATTGCGAGTTCAGAGACAGGTCCCTGGGGACCAAAGAACAATCTCGTTTCAACCCTTGGATGCC TCATTGCTTTGAATGGATTCATTTTTGCTTATAAGCTGATTTACTGAAATCCCATAACCCATCAATGCTG TTAATTTTTTTCTTCCTACCCTTATTACATTCCCTACCCTAAAAGCCTGGGGGAAATACCTGGTTTTGCT TCCCATCTATAATTGAGAAAGAGGGGGGAAAAGATACTGTATTAGAATTTGTGTGATCCTGTGGCACAAT AGATCAACCAACCCATTTAAAGCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4242560 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4242560 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7492 same embryo
	EMAGE:7493 same embryo
	EMAGE:7491 same embryo
	EMAGE:7490 same embryo
	EMAGE:7489 same embryo
	EurExpress:euxassay_005218 same experiment
	MGI:4824566 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source