EMAGE mouse gene expression spatial database

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EMAGE:7490

Tmx2 thioredoxin-related transmembrane protein 2 ( MGI:1914208)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005201. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005201_01	euxassay_005201_02	euxassay_005201_03	euxassay_005201_04

euxassay_005201_05	euxassay_005201_06	euxassay_005201_07	euxassay_005201_08	euxassay_005201_09

euxassay_005201_10	euxassay_005201_11	euxassay_005201_12	euxassay_005201_13	euxassay_005201_14

euxassay_005201_15	euxassay_005201_16	euxassay_005201_17	euxassay_005201_18	euxassay_005201_19

euxassay_005201_20	euxassay_005201_21	euxassay_005201_22	euxassay_005201_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7490_wholemount_strong.wlz

7490_wholemount_moderate.wlz

7490_wholemount_weak.wlz

7490_wholemount_possible.wlz

7490_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7490_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 05 06 07 16 17 18

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22 23

forebrain

moderate

regional

moderate expression: see section 12 14

olfactory lobe

strong

regional

strong expression: see section 06 07 08 09 10 11 13 14 15

hindbrain

strong

regional

strong expression: see section 12 13 14

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 08 09 15 16

midbrain

strong

regional

strong expression: see section 12 13 14

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 06 17 18 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 18 moderate expression: see section 17

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 16 17 18 19 20 21 moderate expression: see section 08

vagus x ganglion

strong

regional

strong expression: see section 06 07 16 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 18 moderate expression: see section 06 07 16 17

trigeminal v nerve

moderate

regional

moderate expression: see section 08 15

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 15

cervical ganglion

moderate

regional

moderate expression: see section 08 15 16

thoracic ganglion

moderate

regional

moderate expression: see section 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 06 07 15 16 17 moderate expression: see section 08

retina

moderate

regional

moderate expression: see section 01 02 21 22 23

lung

strong

regional

strong expression: see section 17 21 moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 18 19 20 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7592
Entity Detected:	Tmx2, thioredoxin-related transmembrane protein 2 ( MGI:1914208)
Sequence:	sense strand is shown >T7592 AAGATGGCTGTCCTTGCGCCTCTGATTGCTTTGGTGTACTCGGTGCCGCGGCTTTCTCGATGGCTGGCCC GACCTTATTGCCTTCTGTCTGCTCTGCTTTCCATTGCTTTCCTCCTCGTGAGGAAACTGCCACCGATTTG CAATGGTCTCCCCACGCAACGCGAAGATGGCAACCCGTGTGACTTTGACTGGAGAGAAGTGGAGATCCTG ATGTTCCTCAGTGCCATTGTGATGATGAAGAACCGCAGATCCATCACTGTGGAGCAACATGTAGGCAACA TCTTTATGTTTAGTAAAGTGGCCAACGCCATCCTTTTCTTCCGACTGGATATTCGAATGGGTCTGCTATA CCTCACACTCTGCATAGTGTTCCTGATGACCTGCAAGCCCCCGCTGTACATGGGTCCTGAGTATATCAAG TACTTCAATGATAAAACCATTGATGAGGAGCTGGAGCGAGACAAGAGGGTCACTTGGATTGTGGAGTTCT TTGCCAACTGGTCTAATGATTGCCAATCCTTTGCTCCCATCTATGCGGACTTGTCCCTCAAGTACAACTG TTCAGGGCTAAATTTTGGGAAGGTAGATGTTGGACGCTACACTGACGTTAGCACACGGTACAAAGTGAGC ACATCACCCCTCACCAGACAGCTCCCTACCCTGATTCTGTTCCAAGGCGGCAAGGAGGTCATTCGTCGGC CGCAGATTGACAAGAAAGGACGAGCTGTCTCTTGGACCTTTTCTGAGGAGAATGTGATTCGAGAATTCAA CTTGAATGAGCTATACCAACGAGCCAAGAAGCACTCAAAGGGTGGAGACATGTCAGAAGAGAAGCCTGTG GACCCTGCTCCCACTACTGTGCCAGATGGGGAAAACAAGAAGGACAAATAGGATCCTGCATTGTTTTTGC TTCCTCTCCTGTCAATTTCAGGAACCTTTGAGGCCCAGTTCCAGCTATAACCACAATCCTTGCCTGAGGC CTTTTGATTTCTGTTTGGCTGTGCCTGGGTAGGGGCACTGTGCCTGCTTCTGATTTTGAAAGAAGCATCC TGGAAACCATGAAGTATTCTACTATGAAGGGCGCATCTGCCCTGGTCAGCTGTTTCTCGAAGGGAGTGAT CTTATCGATAGAGGTAGAAATGGTTTCCCTCCAAGGTTGGGTCAATGTGTAAATTGCTATTCAACACTTG GATATTACCAGTCTGTGGTTTAATTATTCCTCCTAGTGAGCTTGCAAAGCATAGACTAAATTAAACCCTA ATGTTAGATGTCAGTATAAAGCCCTTAGAATTTTTCCTCAAGGACCCCTTCTTCCTTTAGGCCTTTCTGT CTTGATCTGTGCATTAAGAAATTTAAGTTTGGGGCTGGAGAAATGGCCCATCGATTAAGAAAGAGCACTG GTGGCTGCTTTTCCAGAGATCCTGAGTTCAATTCCCAGCAACCACATGATGGTTCATAATGGGATCTGAT GCCCTCTTCTGGCATGCAGGCAGAATACTATATATAGTAAGTAAATCGTAAAGAGAAAGGAATTTAAGGG GTTGGAGAGATGACTCAGTGGCTAAGAGAATTGGCTGTTCTTCCAGAGGACCCAGGTTTGATTCCTAGCA GCACATAGAGGCTAAACCACCTGTAACTCTAGTCTCAGGGGCTCCAACATCCGCCCTTGACCTTGGCTGG CACTGTATGCATGTAGTGCACAAACACGTGACGGAGGAACACATAAAAGGCTTGTATGTGTTATCATTTA AGGTCACTGTTCCCAATTAAAAGCCTTCAACCATGATTTTCATCATTGGAAATAGAATTGAATGGCCACC CTGTGTTGTGGGAACTGAGAAGGGCAATATAAACCTGAGAGGTTCCTTTGTGTGGTGGGGCCTGAAGAAG AAAGCCCCACGGCTGGACAGCTGTTCTCTCCTCCTATCCCACCGCTTCCCCTAATAAAGGGACTGTTCTT TTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4242418 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4242418 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7492 same embryo
	EMAGE:7493 same embryo
	EMAGE:7491 same embryo
	EMAGE:7489 same embryo
	EMAGE:7488 same embryo
	EurExpress:euxassay_005201 same experiment
	MGI:4828837 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source