EMAGE mouse gene expression spatial database

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EMAGE:7492

Cat catalase ( MGI:88271)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004429. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004429_01	euxassay_004429_02	euxassay_004429_03	euxassay_004429_04

euxassay_004429_05	euxassay_004429_06	euxassay_004429_07	euxassay_004429_08	euxassay_004429_09

euxassay_004429_10	euxassay_004429_11	euxassay_004429_12	euxassay_004429_13	euxassay_004429_14

euxassay_004429_15	euxassay_004429_16	euxassay_004429_17	euxassay_004429_18	euxassay_004429_19

euxassay_004429_20	euxassay_004429_21	euxassay_004429_22	euxassay_004429_23	euxassay_004429_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7492_wholemount_strong.wlz

7492_wholemount_moderate.wlz

7492_wholemount_weak.wlz

7492_wholemount_possible.wlz

7492_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7492_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7562
Entity Detected:	Cat, catalase ( MGI:88271)
Sequence:	sense strand is shown >T7562 CCGGGTGGAGACCGCTGCGTCCGTCCCTGCTGTCTCACGTTCCGCAGCTCTGCAGCTCCGCAATCCTACA CCATGTCGGACAGTCGGGACCCAGCCAGCGACCAGATGAAGCAGTGGAAGGAGCAGCGGGCCTCGCAGAG ACCTGATGTCCTGACCACCGGAGGCGGGAACCCAATAGGAGATAAACTTAATATCATGACCGCGGGGTCC CGAGGGCCCCTCCTCGTTCAGGATGTGGTTTTCACTGACGAGATGGCACACTTTGACAGAGAGCGGATTC CTGAGAGAGTGGTACACGCAAAAGGAGCAGGTGCTTTTGGATACTTTGAGGTCACCCACGATATCACCAG ATACTCCAAGGCAAAGGTGTTTGAGCATATTGGAAAGAGGACCCCTATTGCCGTTCGATTCTCCACAGTC GCTGGAGAGTCAGGCTCAGCTGACACAGTTCGTGACCCTCGGGGGTTTGCAGTGAAATTTTACACTGAAG ATGGTAACTGGGATCTTGTGGGAAACAACACCCCTATTTTCTTCATCAGGGATGCCATATTGTTTCCATC CTTTATCCATAGCCAGAAGAGAAACCCACAGACTCACCTGAAGGATCCTGACATGGTCTGGGACTTCTGG AGTCTTCGTCCCGAGTCTCTCCATCAGGTTTCTTTCTTGTTCAGTGACCGAGGGATTCCCGATGGTCACC GGCACATGAATGGCTATGGATCACACACCTTCAAGTTGGTTAATGCAGATGGAGAGGCAGTCTATTGCAA GTTCCATTACAAGACCGACCAGGGCATCAAAAACTTGCCTGTTGGAGAGGCAGGAAGGCTTGCTCAGGAA GATCCGGATTATGGCCTCCGAGATCTTTTCAATGCCATCGCCAATGGCAATTACCCGTCCTGGACGTTTT ACATCCAGGTCATGACTTTTAAGGAGGCAGAAACTTTCCCATTTAATCCATTTGATCTGACCAAGGTTTG GCCTCACAAGGACTACCCTCTTATACCAGTTGGCAAACTGGTTTTAAACAAAAATCCAGTTAATTACTTT GCTGAAGTTGAACAGATGGCTTTTGACCCAAGCAATATGCCCCCTGGCATCGAGCCCAGCCCTGACAAAA TGCTTCAGGGCCGCCTTTTTGCCTACCCGGACACTCACCGCCACCGCCTGGGACCCAACTATCTGCAGAT ACCTGTGAACTGTCCCTACCGCGCTCGAGTGGCCAACTACCAGCGTGATGGCCCCATGTGCATGCATGAC AACCAGGGTGGTGCCCCCAACTATTACCCCAACAGCTTCAGCGCACCAGAGCAGCAGCGCTCAGCCCTGG AGCACAGCGTCCAGTGCGCTGTAGATGTGAAACGCTTCAACAGTGCTAATGAAGACAATGTCACTCAGGT GCGGACATTCTACACAAAGGTGTTGAACGAGGAGGAGAGGAAACGCCTGTGTGAGAACATTGCCGGCCAC CTGAAGGACGCTCAGCTTTTCATTCAGAAGAAAGCGGTCAAGAATTTCACTGACGTCCACCCTGACTATG GGGCCCGCATCCAGGCTCTTCTGGACAAGTACAACGCTGAGAAGCCTAAGAACGCAATTCACACCTACAC GCAGGCCGGCTCTCACATGGCTGCGAAGGGAAAAGCTAACCTGTAACTCCGGTGCTCAGCCTCCGCTGAG GAGACCTCTCGTGAAGCCGAGCCTGAGGATCACCTGTAATCAACGCTGGATGGATTCTCCCACTCCGGAG CGCAGACTCACGCTGATGACTTTAAAACGATAATCCGGGCTTCTAGAGTGAATGATAACCATGCTTTTGA TGCCGTTTCCTGAAGGGAAATGAAAGGTTAGGGCTTAGCAATCATTTAACAGAAACATGGATCTAATAGG ACTTCTGTTTGGATTATTCATTTAAATGACTACATTTAAAATGATTACAAGAAAGGTGTTCTAGCCAGAA ACATGACTTGATTAGACAAGATAAAAATCTTGGCGAGAATAGTGTATTCTCCTATTACCTCATGGTCTGG TATATATACAATACAACACACATACCACACACACACACACATGCAATACACACACTACACACACATACAC ACACTCACACACACTCATACACACACATGAAGAGATGATAAAGATGGCCCACTCAGAATTTTTTTTTTTA TTTTTCTAAGGTCCTTATAAGCAAAACCATACTTGCATCATGTCTTCCAAAAGTAACTTTAGCACTGTTG AAACTTAATGTTTATTCCTGTGCTGTGCGGTGCTGTGCTGTGCTGTGCTGTGCAGCTAATCAGATTCTTG TTTTTTCCCACTTGGATTATGTTGATGTTAATACGCAGTGATTTCACATAGGATGATTTGTACTTGCTTA CATTTTTACAATAAAATGATCTACATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4241333 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4241333 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7493 same embryo
	EMAGE:7491 same embryo
	EMAGE:7490 same embryo
	EMAGE:7489 same embryo
	EMAGE:7488 same embryo
	EurExpress:euxassay_004429 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source