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EMAGE:7496

Gm13023 predicted gene 13023 ( MGI:3650478)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7496
euxassay_005225_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7496Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7496_wholemount_possible.wlz
7496_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7496_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7646
Entity Detected:Gm13023, predicted gene 13023 ( MGI:3650478)
Sequence:sense strand is shown

>T7646
AAGAGGTAATCCCTTGGAGCCTGGAGTCACAGCTTTCCCTTGCCTGAATATGGTGATCTGTGGCCATTGT
CCAGATCAGGATGAGTCTTTAGAAGAAGTCACAGAGGAATGCTATTCCCCACCCACCCTCCAGAATCTGG
CAATTCAGAGTCTACTGAGGGATGAGGCCTTGGCCATTTCTGCTCTCATGGACCTGCCCCAGAGTCTGTT
CCCAGTAATTTTTGAGGAGGCCTTCACTGATGGACATATAGGGATCTTGAAGGCCATGATACCTGAATGG
CCCTTCCCATACCTTTCTTTAGGAAAGCAGATAAATAATTGCAACCTGGAGACTTTGAAGGCTATCCTTG
AGGGACTAGATATACTGCTTGCACAAAAGGTTCAAACCAGTAGGTGCAAACTCAGAGTAATTAATTGGAG
AGATCATGACTTGAACTTATGGGCTGGATCCCATGAAGGTGAAGGCTTATCAGAATTCACTACAGAGAAG
CAGCCAATTGAGAACAGTCCTGGCTGTGAGGTGAAGAAAGAATTGAAGGTGATGACTGAAGTCCTTCGCA
TGAAGGGCAGACTTGATGAATCTACCACATACTTGTTGCAGTGGGCCCAGCAGAGAACAGATTCTATTAA
TTTATTCTGCAGAAAGCTAGTAATTGAAGGCTTAACCAAAGCCTCAGTGATAGAAATCTTCAAAACTGTA
CACGCAGACTGTATACAGGAGCTTATCCTAAGACGTATCTGCATAGAAGAGTTGGCTTTTCTTAATCCCT
ACCTGAAACTGATGAAAAGACTTTTCACACTCACACTAGATCACATCATAGGTACCTTCAGTTTTGGTGA
TTCTGAAAAGCTTGATGAGGAGATAATATTCAGCTTGATTTCTCAACTTCCCACACTCCACTGTCTCCAG
AAACTCTATGTAAATGATGTCCCTTTTATAAAAGGCAACCTGAAAGAATACCTCAGGTGCCTGAAAAAGC
CCTTGGAGACACTTTGCATCAGTAACTGTGACCTCTCACAGTCAGACTTGGATTACCTGCCCTATTGCCT
GAATATTTGTGAACTCAAACATCTGCATCTTAGTGATATATGTCTACGTGATTTACTCCTTGAGCCTCTT
GGGTTTCTCCTTGAGAGAGTTGGAGATACCCTGAAAATCCTGGAATTGGAGTCATGTTGTATAGTGGACT
TTCAGTTCAGTGCCTTGCTGCCTGCCCTAAGCCAATGTTCTCACCTCAGAGAGGTCACTTTCTATGATAA
TAATTTCTCTCTGCCTTTCTTGAAACAACTTCTACACCACACAGCCCTGCTGAGTCAGCTGATCAGTGAG
TGTTACCCTGCCCCTCCGGAGTGCTATGATGACAGTGGTGTAATACTAACACACAGATTAGAAAGTTTTT
GTCCTGAGCTCCTGGATATACTCAAAGCCAAAAGACAGCTCCAAAGTGTCTCCTTTGAAACAACCCAATG
CTCTAAATGTGGTGGGTGCTACATTTATGACCAGGAGACCCAATGTTGTCATTTTGTGGAACTACTATTA
GCTTGATTGCGAAACCGAGAAATGGAAACTTAGTATTGGGGACTGATGAAATCCTAAGTAAACGTCCACT
GCTAAATGGAGCATGAAAATGTCAATCACCTAAAAGTCTGAGATACATAGGAAAGTCAATACCTTCCTCA
GAGCTGGTGAATGGATGTTGCATCTGCAGAAAGTATCAAGCGCTTGTAGTTTGTGTGTGTTACAATAGAG
GCACCATTTTATGAAACTGGCCCAATCTGTTTTCTGTGTACAATAAATGTGTTGACTTAAAAAAAAAAAA
AAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:30478700 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30478700 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7497 same embryo
 EMAGE:7495 same embryo
 EMAGE:7498 same embryo
 EMAGE:7499 same embryo
 EMAGE:7494 same embryo
 EurExpress:euxassay_005225 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS