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EMAGE:7525

Elf5 E74-like factor 5 ( MGI:1335079)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7525 EMAGE:7525 EMAGE:7525 EMAGE:7525 EMAGE:7525
"Pseudo-wholemount" of euxassay_004671. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004671_01 euxassay_004671_02 euxassay_004671_03 euxassay_004671_04
EMAGE:7525 EMAGE:7525 EMAGE:7525 EMAGE:7525 EMAGE:7525
euxassay_004671_05 euxassay_004671_06 euxassay_004671_07 euxassay_004671_08 euxassay_004671_09
EMAGE:7525 EMAGE:7525 EMAGE:7525 EMAGE:7525 EMAGE:7525
euxassay_004671_10 euxassay_004671_11 euxassay_004671_12 euxassay_004671_13 euxassay_004671_14
EMAGE:7525 EMAGE:7525 EMAGE:7525 EMAGE:7525 EMAGE:7525
euxassay_004671_15 euxassay_004671_16 euxassay_004671_17 euxassay_004671_18 euxassay_004671_19
EMAGE:7525 EMAGE:7525 EMAGE:7525 EMAGE:7525 EMAGE:7525
euxassay_004671_20 euxassay_004671_21 euxassay_004671_22 euxassay_004671_23 euxassay_004671_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7525Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7525_wholemount_strong.wlz
7525_wholemount_moderate.wlz
7525_wholemount_weak.wlz
7525_wholemount_possible.wlz
7525_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7525_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5764
Entity Detected:Elf5, E74-like factor 5 ( MGI:1335079)
Sequence:sense strand is shown

>T5764
CCACGCGTCCGGCGCGCTTGCCTTCTCTTGCCTTGAAAGCCTTCTGTCTGGACCTAGCCACCACTTGTCT
TCACGGTGATGTTGGACTCCGTAACCCATAGCACCTTCCTGCCCAACGCATCCTTCTGTGACCCCCTGAT
GCCTTGGACCGATCTGTTCAGCAATGAAGAATACTACCCTGCCTTTGAGCATCAGACAGCCTGTGATTCC
TACTGGACATCAGTGCACCCTGAATACTGGACCAAGCGCCACGTCTGGGAATGGCTCCAATTCTGCTGCG
ACCAGTACAAGCTTGATGCCAACTGCATCTCCTTCTGTCACTTCAACATCAGCGGCCTGCAGCTCTGCAG
CATGACGCAGGAGGAGTTCATTGAGGCAGCCGGCATCTGTGGGGAGTACCTGTACTTCATTCTCCAGAAC
ATTCGCTCGCAAGGTTACTCCTTTTTCAATGATGCTGAAGAAACCAAGACTGGCATCAAAGACTATGCTG
ATTCCAGTTGCTTGAAAACAAGTGGCATCAAGAGTCAAGACTGTCACAGCCGAACAAGCCTCCAAAGTTC
TCACCTGTGGGAATTTGTCAGAGACTTGCTGCTGTCCCCTGAAGAGAACTGTGGCATCCTGGAATGGGAA
GACAGGGAGCAGGGCATTTTCCGAGTGGTTAAGTCAGAAGCCCTGGCAAAGATGTGGGGACAAAGGAAGA
AGAATGACAGGATGACGTACGAGAAGCTGAGCCGAGCCCTGAGATACTACTATAAAACGGGAATTCTGGA
GCGGGTTGACCGGAGGTTAGTGTACAAATTTGGAAAGAACGCGCACGGGTGGCAGGAAGAGAAACTCTGA
TGGACACCGGACACCAGGCTCATTTGATGGATTTCTGTTGTTTGAAACAATCAGATCAAACTAGGCATTT
GAAAGTCTCCCTCCTCCTCCTCCTCCCCCTCCTTCCCCTCCTCTTCTTCCTCCCCCTCCTCCTCTTCAAA
ACCTACAAACACACTGATAAAATTTCTGCATGTCTCAGCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:2651428 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2651428 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7527 same embryo
 EMAGE:7526 same embryo
 EMAGE:7524 same embryo
 EMAGE:7523 same embryo
 EMAGE:7528 same embryo
 EurExpress:euxassay_004671 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS