EMAGE mouse gene expression spatial database

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EMAGE:7526

Coch coagulation factor C homolog (Limulus polyphemus) ( MGI:1278313)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004668. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004668_01	euxassay_004668_02	euxassay_004668_03	euxassay_004668_04

euxassay_004668_05	euxassay_004668_06	euxassay_004668_07	euxassay_004668_08	euxassay_004668_09

euxassay_004668_10	euxassay_004668_11	euxassay_004668_12	euxassay_004668_13	euxassay_004668_14

euxassay_004668_15	euxassay_004668_16	euxassay_004668_17	euxassay_004668_18	euxassay_004668_19

euxassay_004668_20	euxassay_004668_21	euxassay_004668_22	euxassay_004668_23	euxassay_004668_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7526_wholemount_strong.wlz

7526_wholemount_moderate.wlz

7526_wholemount_weak.wlz

7526_wholemount_possible.wlz

7526_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7526_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 04 05 06 weak expression: see section 18 19 20

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 15 16 17 18

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 03 04 05 06 07 19 20

pons mantle layer

moderate

regional

moderate expression: see section 07 08 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 08 16 17 weak expression: see section 03 04 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 07 08 17

ventral grey horn

moderate

regional

moderate expression: see section 11 12 14 15 weak expression: see section 10

cochlea

strong

regional

strong expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 weak expression: see section 11 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7692
Entity Detected:	Coch, coagulation factor C homolog (Limulus polyphemus) ( MGI:1278313)
Sequence:	sense strand is shown >T7692 GGCGGATCCCACGCAGGTCCACGGAGATCCTCGCCATGCCCTCGTCCAGGATCCCTGCTCTCTGCCTCGG TGCGTGGCTGCTGCTGCTGCTGCTGCCCCGGTTCGCGCGCGCCGAGGGAGCGGTTCCCATTCCTGTCACC TGCTTTACCAGAGGCCTGGATATCCGAAAAGAGAAAGCAGATGTTCTCTGCCCAGGAGGCTGCTCTCTTG AGGAATTCTCTGTGTTTGGGAACATAGTGTATGCGTCAGTGTCCAGCATCTGCGGCGCTGCTGTCCATAG GGGAGTGATTGGCACCTCAGGGGGACCTGTGCGTGTCTACAGCCTTCCTGGTCGAGAGAACTACTCCTCG GTAGATGCCAACGGCATCCAGTCTCAGATGCTTTCCCGATGGTCCGCGTCCTTCGCTGTGACCAAAGGCA AAAGCAGTACCCAGGAAGCCACAGGACGGGCAGTGTCCACAGCCCACCCACCTTCAGGTAAAAGACTAAA GAAGACACCAGAGAAGAAGACTGGCAACAAAGACTGTAAGGCAGACATTGCATTTCTCATTGATGGAAGC TTCAATATTGGGCAGCGCCGATTTAATTTGCAGAAGAATTTTGTTGGGAAAGTGGCACTAATGTTGGGAA TTGGAACAGAAGGACCACACGTGGGTCTCGTTCAAGCCAGTGAACACCCCAAAATAGAATTTTACTTGAA AAACTTTACTTCAGCCAAAGATGTCTTGTTTGCCATAAAAGAAGTAGGTTTCCGAGGGGGTAACTCCAAC ACAGGAAAAGCCTTGAAGCACACTGCTCAGAAATTCTTTACAGCAGACACTGGTGTGAGAAAAGGAATAC CAAAAGTGGTGGTAGTGTTTATTGATGGTTGGCCCTCTGATGACATTGAGGAAGCAGGCATTGTGGCCAG AGAGTTTGGTGTCAATGTATTTATAGTTTCTGTGGCCAAGCCCATTCCTGAAGAACTGGGGATGGTTCAA GATGTTGCATTTGTTGACAAGGCTGTGTGTCGGAATAATGGCTTCTTCTCTTATCACATGCCCAACTGGT TTGGCACTACAAAATATGTGAAGCCTCTGGTGCAGAAGCTCTGTACGCACGAACAGATGATGTGCAGCAA AACCTGCTACAACTCAGTGAACATTGCCTTTCTGATTGACGGCTCCAGCAGTGTTGGAGATAGCAATTTC CGCCTCATGCTAGAATTTGTTTCTAACATAGCGAAGACATTTGAAATCTCAGACATTGGAGCCAAGATAG CTGCTGTACAGTTCACTTATGACCAGCGCACCGAGTTCAGTTTCACTGACTATAATACCAAAGAGAACGT CCTAGCTGTCCTAGCGAACATCCGCTACATGAGTGGTGGCACAGCTACTGGTGATGCCATCGCCTTTACT GTTAGAAATGTATTTGGTCCCATAAGGGACAGCCCCAACAAAAACTTCCTGGTTATTGTCACAGATGGGC AGTCCTATGATGATGTCCGAGGCCCTGCTGCAGCTGCCCATGATGCAGGTATCACCATCTTCTCTGTTGG TGTGGCTTGGGCACCGCTGGATGACCTGAGAGATATGGCCTCTAAACCCAAAGAGTCACACGCTTTCTTT ACCAGAGAGTTCACAGGGTTAGAACCAATTGTCTCTGACGTCATCAGAGGCATTTGTAGAGACTTCTTAG AATCCCAGCAATAACCGATACTCTGACAACTCAAGGAATACGTGCAAGGGGATCTAATGTGCAAATTATA TTCTCAATGCCTATGTAACTTTATAGCTTACCAGTGTCAAAAAATGCGTCCACAGCTGTTTAAAGCAAAT GAATATTCATGTGATGCTCACAATTTAGATTGGCCGAGACTTGATAATCAGGCCCTTAGAAACTCAGGAA AGAAGAGTTGTCATGGATTAACATTGGGAGTTCAAATATGCATTCAAGTGGATAGGTAAGCTACACAGCT CAATAAAAGAACCTGGCGCTTACACACAAAGCACTGTTCCCTCTTTAATCACTCTGCATTGACCATGCAA GGAAAACAGAACAGCTTTTAAACACAGATCAAGTATACATATTTTGACCCATGTGGATGTTTTCTTAAAA CCAGCCAAGAACAGACAGCTGTTATTATGTGCACTAGCCATAACTACACATTATATGGAATCATATATCA AGCTTCTTTTGTAGTGTGTTTTCATAACTTGATGGCTGAAATACCACACTGAGTAAAGGTAGGATTGCCT GGTATTTTTCTATTTATATCCTTAATTTTATGTGTATAGACAGGCATGTACTCCGAGGACTAAGAAAATG TTTAAGCAGATAACTTTTTTTTTTTGAAAAAAAAGATGTGTCAAGTATTGTAACCGAAAAAATACACAGC TTAATAGCTTGGCTGTCAGCAATAAAACTGCTAGTGACTAAGTGCCTATTTAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6306601 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6306601 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7527 same embryo
	EMAGE:7525 same embryo
	EMAGE:7524 same embryo
	EMAGE:7523 same embryo
	EMAGE:7528 same embryo
	EurExpress:euxassay_004668 same experiment
	MGI:4823969 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source