EMAGE mouse gene expression spatial database

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EMAGE:7541

Cog1 component of oligomeric golgi complex 1 ( MGI:1333873)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004669. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004669_01	euxassay_004669_02	euxassay_004669_03	euxassay_004669_04

euxassay_004669_05	euxassay_004669_06	euxassay_004669_07	euxassay_004669_08	euxassay_004669_09

euxassay_004669_10	euxassay_004669_11	euxassay_004669_12	euxassay_004669_13	euxassay_004669_14

euxassay_004669_15	euxassay_004669_16	euxassay_004669_17	euxassay_004669_18	euxassay_004669_19

euxassay_004669_20	euxassay_004669_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7541_wholemount_strong.wlz

7541_wholemount_moderate.wlz

7541_wholemount_weak.wlz

7541_wholemount_possible.wlz

7541_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7541_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8641
Entity Detected:	Cog1, component of oligomeric golgi complex 1 ( MGI:1333873)
Sequence:	sense strand is shown >T8641 CCTGGCTTAGCACCATGGCGGCCGCGACCGCCTCCTCCGCGCTCAAGCGGCTGGATCTGCGCGATCCCAA CGCGCTCTTCGAGACTCATGGAGCGGAGGAGATCCGCGGGCTGGAGCGCCAGGTCCGAGCCGAGATCGAA CACAAGAAGGAGGAGCTGCGGCAGATGGTGGGCGAGCGGTACCGCGACCTGATCGAGGCGGCGGACACCA TCGGCCAGATGCGCCGCTGCGCCGAGGGCCTGGTGGACGCCGTGCAGGCCACCGATCAATACTGCGCCCG CCTCCGCCAGGCCGGCTCCGTGGCGCCCCGGGTCCCACGGGCCCCGCAGCCACAGCCGCCGTCCGAGAAG TTCTACAGCATGGCCGCCCAGATTAAGCTGCTGCTGGAGATCCCGGAGAAGATCTGGAGCGCCATGGAAG CCTCGCAGCACCTCCAGGCCACACAGCTCTACCTGCTCTGCTGCCACCTGCATAGCCTGCTGCAGCTGGA CTCCTCTAACTCCAGATACAGCCCCATCCTCTCGAGATTTCCAATCCTCATCCGCCAGGTGGCAGCAGCC AGCCACTTCCGGTCAACTATTTTGCACGAAAGCAAGATGCTGCTCAAATGCCAGGCTGTGTCGGACCAGG CTGTGGCCGAAGCCCTCTGCTCCATAATGCTCTTGGAGGAGAGCTCTCCGCGCCAAGCCCTCACAGACTT CCTGCTGGCCAGAAAGGCGACTATTCAGACGCTCCTGAACCAGTCTCATCACGGTGCTGGCATCAAGGCC CAGATTTGCTCTTTGGTGGAGCTGCTGGCTACCACTCTGAACCAGGCACATGCCCTTTTCTACACCCTAC CAGAAGGCGTGCTGCCCGACCCATCCCTGCCCTGTGGCTTGCTCTTTTCTACCCTGGAGACAGTCACAAG GCAGCATCCCACTGGAAAGGGCATCGGTGCCCTCCAAGGGGAGATGAAGCTATGCAGCTGGTTCAGACAC CTGCCCACCTCCATTATCGAGTTCCAGCCCACACTCCGAACTCTCGCACATCCCATCAGCCAGGAGTACC TGAAAGACACGCTGCAGAAATGGATCGACATGTGCAATGAAGACATTAAAAATGGGATCGGCAATCTGCT TATGTACGTGAAGAGCATGAAGGGTCTGGCAGGCATCAGAGACGCCATATGGGATTTGCTTAGCAACGAG TCTGCCAGCCACAGCTGGGAGGTGGTGTGTCAGCGGCTTCTGGAGAAGCCGCTCTTGTTCTGGGAGGACC TGATGCAGCAGCTCTTCCTGGATCGGTTACAGACACTGACCAGAGAAGGCTTTGAATCCATCTCCAACAG CTCCAAAGAGCTTCTAGTCTCAGCTCTGCAGGAGCTGGAGACCAACAACTCCACATCGAACAAGCATGTC CACTTTGAGCAGAACATGTCTTTCTTCCTCTGGTCCGAGAGCCCCAACGACCTGCCTTCTGATGCTGCCT GGGTCAGCGTAGCAAACCGGGCTCAGTTTGCCAGTAGTGGCCTCTCCATGAAAGCCCAAGCCATTAGCCC GTGTGTCCAGAACTTCTGTTCTGCCCTGGATTCTAAGCTGAAGGTTAAACTGGACGACCTCCTGGCCTAC CTTCCCTCCAGTGACACACCATTGCTCAAGGATACCACCCCCACGCACCAGCCTAAGAACTCTGCCTTTG ATCGATACGCAGATGCTGGGACCGTGCAGGACATGCTGCGTACGCAGTCCGTGGCCTGCATCAAGTCCGT CGTGGGCTGCATCCAGGCAGAGCTGTGCACCATTGAAGAAGTCACACGGGAACAAAAGGATGTCCTTCAC AGCACCAAGCTGCACGCAGTCCTCTTCATGGCCAGACTCTGCCAGTCTCTGGGAGAGCTGTGCCCTCACC TAAAGCAGTGTGTTGTGGGACAGTGCGGGGGTTCTGAGAAACCTGCCAGGGAGGCGAGGGCTCTGAAAAA GCAGGGGAAGGGGAGAGCTCAGGATGTCCTCCCCGCGCAGGCCCAGTGGCAGGGAGTGAAGGAAGTCCTG CTCCAACAGAGCGTCATGGCCTACCGGGTCTGGAGCACAGCACTTGTGAAATTTCTGATTTGTGGATTCA CTCGATCACTGCTTCTCAGGGATGCCGGCTCAGTCCTGGCCACTGCCACCAATTGGGATGAACTAGAAAT TCAAGAGGAGACAGAGTCTGGCAGCAGTGTCACGTCTAAGATCCGCCTCCCTACTCAGCCCTCCTGGTAT GTACAGTCCTTCCTGTTTAGCTTATGCCAGGAAGTTAATCGGGTTGGAGGCCATGCTTTGCCAAAGGTCA CCCTACAGGAGATGCTGAAGACCTGCATGGCCCAAGTCATAGCTGCCTATGAGCAACTCACAGAGGAAAA CCAGATAAAGAAAGAAGGGGCATTTCCAATGACCCAGAACCGAGCCCTGCAGCTCCTCTACGACCTACGC TACCTCACCATGGTTCTGAGCAGCAAGGGGGAGGAGGTGAAGAGTGGCCGGAGCAAGGCAGACTCCAGAA TGGAGAAGATGACCGAGAGGCTGGAGGCCCTCATTGACCCTTTTGACCTGGATGTCTTCACACCGCATCT CAACAGCAACCTCAACCGCCTGGTGCAGCGGACTTCAGTTCTATTTGGCTTGGTTACAGGCACAGAGAAT CAGTTTGCCTCCCGGAGCAGCACTTTCAACTCCCAGGAGCCCCATAACATACTGCCGCTGGCCTCCAGTC AGATCAGGTTTGGACTCCTTCCACTGAGCATGACAAGCACAAGAAAGGCGAGGGCAACCAGTAGAAGCGT TGAAACCCAAGCCCAGGTTGGCCCCCCGGCACTCTCCAGAGTAGGTGACCCGACGACGCATCCTGGCTCC TTATTCAGGCAGCTTGCAAGCGAGGAAGACGACTCACCTGCACCTTCATTATTCAAGCTTGCCTGGCTCT CTAGTATGACCAAGTAGCATAGGAACAATGCCCAGGCTCTCTAAATAAATACTAGCGCATTGTTTTTCCT AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5720460 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5720460 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7542 same embryo
	EMAGE:7544 same embryo
	EMAGE:7545 same embryo
	EMAGE:7546 same embryo
	EMAGE:7543 same embryo
	EurExpress:euxassay_004669 same experiment
	MGI:4823970 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source