EMAGE mouse gene expression spatial database

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EMAGE:7546

Rnf14 ring finger protein 14 ( MGI:1929668)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004677. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004677_01	euxassay_004677_02	euxassay_004677_03	euxassay_004677_04

euxassay_004677_05	euxassay_004677_06	euxassay_004677_07	euxassay_004677_08	euxassay_004677_09

euxassay_004677_10	euxassay_004677_11	euxassay_004677_12	euxassay_004677_13	euxassay_004677_14

euxassay_004677_15	euxassay_004677_16	euxassay_004677_17	euxassay_004677_18	euxassay_004677_19

euxassay_004677_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7546_wholemount_strong.wlz

7546_wholemount_moderate.wlz

7546_wholemount_weak.wlz

7546_wholemount_possible.wlz

7546_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7546_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pons mantle layer

moderate

regional

moderate expression: see section 04 13

facial vii ganglion

moderate

regional

moderate expression: see section 01 02 15

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 03 04 14 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 01 02 03 04 05 12 13 14 15 16 17

vagus x ganglion

moderate

regional

moderate expression: see section 05 14

trigeminal v nerve

moderate

regional

moderate expression: see section 05 12

dorsal root ganglion

strong

regional

strong expression: see section 04 05 06 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8646
Entity Detected:	Rnf14, ring finger protein 14 ( MGI:1929668)
Sequence:	sense strand is shown >T8646 GGCGGTGCTCAGGTGCTGCGGCTGGTCTGCAGGCCTGGCCGGCAAGCGTCTGCCGAGGCCCAGTCAAGGG CATCCTGCGGAACTTTAGGGCCCGGCTCGGCTGAGCGGCCGGAAGATGGCAGACTTCCTGTAGTATATCG GACGCAAATCTCACATCTTGGTTTCTTATCCAACTTCAGTCTAACTTCACTGGCTAGAAAATAAATGGGA TTTGCACAAATTCCAAGCCAAAGTGAAATACAGATTGCAGCAGCATTAATATCCTTTTCTCCTGGTTGTT ACTAACAGCAAGTTCAATGTCGGCAGAAGACCTGGAAGCCCAGGAGGATGAACTACTGGCCCTGGCGAGT ATTTATGATGCGGATGAATTTAGAAAAGCTGAGTCTGTCCAAGGAGGAGAAACCAGGATCTATTTGGATC TGCCACAGAATTTTAAGATATTCGTGAGCGGCAATTCAAATGAGTCTCTCCAGAATAGTGGCTTTGAATA CACCATTTGCTTTCTGCCACCACTTGTGCTGAACTTTGAACTGCCACCAGATTATCCATCCTCCTCCCCA CCTTCATTCACACTTAGTGGCAAATGGCTGTCACCAACTCAGCTCTCTGCTCTCTGCAAGCACCTAGACA ACCTGTGGGAAGAACACCGAGGCAGAGTGGTGCTGTTTGCCTGGATGCAGTTTCTTAAGGAGGAGACCCT CACCTACCTGAATATTGTCTCTCCTTTTGAGCTCAAGATGGGGTCTCAGAAAAAAGTTCAGAGAAGGGCA ACAGCCCAGGCCTCTTCCAGCACAGAGCTTGGTGTGGGAGGAGCTGCTGCGGCGGATGTAGACCAAGAAG AAACTGTGGATGAGAGAGCTGTACAGGATGTAGAGTCATTGTCAAGTCTGATCCAGGAGATCTTGGACTT CAATCAAGCTCGGCAGACGAAGTGCTTCAATAGTAAACTGTTCTTGTGCAGCATCTGTTTCTGTGAGAAG CTGGGTAGTGATTGCATGTACTTCTTGGAGTGCAAGCATGTATACTGCAAAGCCTGTCTGAAGGATTACT TTGAAATCCAGATCAAAGATGGTCAAGTTAAATGCCTCAACTGTCCAGAGCCACAGTGCCCTTCAGTGGC GACTCCTGGTCAGGTCAAAGAGCTGGTAGAAGCAGACTTGTTTGCTCGCTATGACCGTCTCCTCCTGCAG TCTACCTTGGATCTAATGGCAGATGTGGTGTATTGTCCCCGTCCATGCTGCCAGCTCCCTGTGATGCAAG AGCCTGGTGGTACCATGGCTATCTGCTCCAGCTGCAATTTTGCCTTCTGTACCTTATGCAGACTGACCTA TCACGGGCTTTCTCCATGTAAGGTAACTGCAGAGAAATTAATAGACTTACGAAATGAATACCTACAAGCA GATGAAGCTACTAAGAGGTTCTTGGAACAGAGGTATGGCAAGAGGGTGATTCAGAAGGCCCTGGAGGAGA TGGAAAGTAAAGACTGGCTGGAGAAGAACTCCAAGAGCTGCCCCTGCTGTGGCACACCCATACAGAAATT AGACGGCTGCAACAAGATGACTTGCACTGGCTGTATGCAGTATTTCTGCTGGATTTGCATGGGTTCTCTC TCTCGAGCAAATCCTTACAGACATTTCACTGATTCTGAGTCTCCATGCTTTAACAGGTTGTTCCATGCCG TGGATATTAATGGTGATATGTGGGAAGATGAAATTGAGGAGGACGACGATGATGAAGACGACGACGACGA CTAGTTCATTCCTTCTGCTCACAATATAGAAGTGGAATGGTTTGCCCTGATCTTCTGTCAAGTACACAAA GAGATCTTGCAGGATATTTAGGGGCCGTCTATTCACTCTTCCAGTGTAGAAGGAAGAACAGGGTTATATT TTCCTTTGGTTCTACTGATTAAGGCACATACATTTTCCATGTAACATAATCTGATAAAATTATATGCCAA AGGATCAGAAATGAAAACTATAGGAGATTAAATATTATAATATGCCCAAACTGTGAATAGTTAAAAAGTA AATATTTATTTTCTCCCCCAAGCTTTAGGTTAGGGAGAGGGTCAAGAGTTTAGCTTTTAGACCATGTCCA AGCAGCCTCACGTTGTTGGAGCACTCTCAGTTCCATCCCTCAGCTGGGGACAGTCACAGCCCCAGCCACA CTGTCCTGGGAGCCTGATCCATTCTCTGTACACTGCTCACTCCCATCCGTGCTGTGGAGGAGCCTGTGCT CTGTAACTGACCAGTTGTCCCAGTGTCGTTCTGTTCTAGTGCTTGCCAGAATAGAAGTCTATAGTACATT AAAAGTATTTTTAGTGCCACAATCCTTGTGGGTCACTTGATATTTATGAATAGTGTATTGAATCCACTGT TTTAGAGCCAAATGTAGAATTTAGGATTCTGGCTCAGAATTCCTCCAGATACAGAGTCAGAGCTGTGTCT GCCCCATTGTGTTCTCAACTTTCTCCCTTTGCCTCCCACCTCTTGTGGGCAAGGACAGTTTTAATGTTGA TCCCAGCAGTGTGTGTCTTTATTTCCTCACTAGAGTAGGAAAAAGCCCAGTGTATTCCTCATGAATGCTC TCTAAACCTGTGAAGAGCATCATGGCTCTACCCTGACCTTCCGAACCCTCGGGTCCTCTCTAGTCACCTA CAGCAACTGGGCTTCCTTCCCTCCCTTCCCCTCCCCTCGTAAACAGCAGTGTCTCGAGTGATAATAGTAG CTTGATAGCTGGATTCCTAATTCTGAGTTTTATTGGCTTTTTGAAAAACTGTCTTCTTCATGATTCGGTA AGTGGCTTGGTAGTTACTTGGTGGATGTTTTGAAGGGACAGTAATTCACCTGTAGCTTGTGAACATGTGT TTTCTACTTTGACACTGAAAATTAAGGAATTGCTTAACTGTCAGCTCAGACTGCTTCTGGATTGTCAATG AGGATGCTTAATCTGAACAATAAAGTAATTTAAGATCATCCTGTAATTGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5720753 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5720753 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7542 same embryo
	EMAGE:7541 same embryo
	EMAGE:7544 same embryo
	EMAGE:7545 same embryo
	EMAGE:7543 same embryo
	EurExpress:euxassay_004677 same experiment
	MGI:4827763 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source