EMAGE mouse gene expression spatial database

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EMAGE:7554

Slc6a2 solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 ( MGI:1270850)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004595. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004595_01	euxassay_004595_02	euxassay_004595_03	euxassay_004595_04

euxassay_004595_05	euxassay_004595_06	euxassay_004595_07	euxassay_004595_08	euxassay_004595_09

euxassay_004595_10	euxassay_004595_11	euxassay_004595_12	euxassay_004595_13	euxassay_004595_14

euxassay_004595_15	euxassay_004595_16	euxassay_004595_17	euxassay_004595_18	euxassay_004595_19

euxassay_004595_20	euxassay_004595_21	euxassay_004595_22	euxassay_004595_23	euxassay_004595_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7554_wholemount_strong.wlz

7554_wholemount_moderate.wlz

7554_wholemount_weak.wlz

7554_wholemount_possible.wlz

7554_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7554_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

regional

strong expression: see section 06 09 15 moderate expression: see section 07 08 14 16

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 09 15

pons mantle layer

strong

regional

strong expression: see section 07 08 16 17 18

trigeminal v nerve maxillary division

moderate

regional

moderate expression: see section 04 05 06 20 21

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 10 16

cervical ganglion

strong

regional

strong expression: see section 08 09 16 17

thoracic ganglion

strong

regional

strong expression: see section 10 11

extrinsic ocular muscle

moderate

regional

moderate expression: see section 05 06 19 20

naris

moderate

regional

moderate expression: see section 04

lower lip

moderate

regional

moderate expression: see section 14 15 16 18 weak expression: see section 07 10 11 12

upper lip

moderate

regional

moderate expression: see section 08 09 14 15 16 17 18 weak expression: see section 07 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7609
Entity Detected:	Slc6a2, solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 ( MGI:1270850)
Sequence:	sense strand is shown >T7609 GCCAAACAACACTGCCCCAAAGGCCCAGCCTGCAGGCCACCAGTCACAATCCTTATCTGAAGCACCAAGT TTCCCTGTTGCTGGTGGAGAAAACTCTCACCAAAACCTCCGAAGTCCCTCTCTCCAGCCGCACCCATGCT TCTGGCGCGAATGAATCCGCAGGTGCAGCCGGAGCTCGGCGGGGCGGACCCGCTGCCTGAGCAGCCCCTG AGGCCCTGCAAAACCGCCGATCTACTAGTGGCGAAGGAACGCAACGGAGTCCAGTGCCTCTTGGCGTCCC AGGACAGCGACGCTCAGCCCCGGGAGACTTGGGGCAAGAAGATTGATTTCCTGCTGTCCGTGGTGGGCTT CGCTGTGGACCTGGCCAACGTGTGGCGGTTCCCTTATCTCTGCTACAAGAATGGTGGTGGTGCCTTCCTG ATTCCATACACGCTGTTCCTCATCATTGCTGGGATGCCTCTGTTTTACATGGAGCTGGCTCTGGGGCAAT ACAACCGGGAGGGGGCAGCCACAGTGTGGAAGATCTGCCCTTTCTTCAAAGGAGTGGGCTATGCTGTGAT CCTCATTGCCCTCTATGTCGGCTTTTACTACAATGCCATCATCGCCTGGTCACTCTACTACCTCTTTGCA TCCTTCACCTTGAACCTGCCCTGGACCAACTGCGGACACTCCTGGAACAGCCCCAACTGTACGGACCCCA AACTCCTCAACGCCTCGGTGCTGGGTGACCATACCAAATACTCCAAATACAAGTTCACACCAGCTGCGGA GTTTTATGAACGCGGAGTCCTGCACTTGCACGAGAGCAGTGGGATCCATGACATCGGCCTGCCTCAATGG CAGCTGCTGCTCTGTCTGATGGTCGTCATCGTTGTCTTGTATTTCAGCCTCTGGAAAGGAGTGAAGACAT CAGGAAAGGTTGTCTGGATCACAGCCACCCTGCCTTACTTTGTCTTGTTTGTGCTCCTGGTCCATGGCGT CACACTGCCCGGTGCCTCCAATGGCATCAATGCCTACTTGCACATTGACTTCTACCGCCTCAAGGAGGCC ACGGTATGGATTGATGCTGCAACCCAGATATTTTTTTCTCTGGGGGCTGGATTTGGAGTCTTGATTGCAT TTGCCAGTTACAATAAATTTGACAACAACTGTTACAGGGATGCTCTACTGACCAGCACCATCAACTGTGT TACCAGTTTTATTTCTGGATTTGCCATCTTCTCCATCCTTGGTTATATGGCCCATGAACATAAGGTCAAT ATTGAGGATGTTGCCACTGAAGGAGCTGGGCTTGTGTTTATCCTGTATCCAGAAGCCATCTCTACTCTGT CAGGATCCACGTTCTGGGCCGTCCTGTTCTTCTTGATGCTCCTGGCTCTGGGGCTAGATAGTTCAATGGG AGGCATGGAAGCAGTCATCACTGGACTGGCTGATGACTTCCAGGTCCTGAAGCGACACCGGAAACTCTTC ACGTGTGTTGTCACCATCAGCACCTTCCTTCTGGCATTGTTTTGCATAACAAAGGGAGGGATTTATGTCC TCACTCTGCTGGACACCTTTGCAGCAGGCACCTCCATTCTGTTTGCGGTGCTTATGGAAGCTATTGGAGT TTCCTGGTTTTATGGTGTGGACAGGTTCAGCAATAACATCCAGCAGATGATGGGGTTTAAGCCAGGCCTG TACTGGAGGCTGTGTTGGAAGTTCGTGAGCCCCGCCTTCCTCCTGTTTGTGGTGGTGGTCAGCATCATCA ACTTCAAGCCGCTTACCTATGATGACTACACCTATCCTCCCTGGGCCAACTGGGTCGGGTGGGGCATCGC CCTCTCATCCATGATCCTGGTCCCTGCCTATGTCATCTACAAGTTCCCCAGCATACGCGGCTCCCTGTGG GAGAGAGTGGCCTACGGAATCACCCCGGAGAATGAGCACCACCTGGTGGCCCAGAGAGACGTGAGACAGT TCCAGTTGCGGCACTGGCTGGCTATCTGAACCTGGCAATGTGGAGGAGGAGGAGGAACCCATGTGCCAAC GTCCGGGTCCAGGGCACCCTCTTTGCCCCCATTCGGATACCGCCTTGGGATTCCTCTCCTGGAAGACACC TTCCCCGTCGCTCCCGTTTTCTCATTTACTAATGATTCCGTGACGCTGGTTTTGTTCACCTTCTGTTCAT CTGGCCTGGAGGCAAGCGAGAGTGGAGAACTGGTGAGCCTCAAGGAAGAGGCTGGAAGGAGGAAGTGGGA CGTGAATTCTGGAACTCTGGCTTGAGGGCAGGTTTACTGCATTTGGAGCACACCTCAGCTGAGGTCCACA GCCCGGGCAGAAACCCCTCCCGGATTTGCTTGTACTTCAGATCATAAACAAAATCTTCCATTCACCCAGG CTGAGAGGAGATTGGCTTCTGGTTAGATATCAAAAGCCCAAGCAAAGCCTGACAGGGTCTGTGTGGGGGC CAAGGTGTGCAGGTTACCCCTCTCTTTCCATTTATCCTGTCACCGCTCCAGCATCTGAGCCTCTGTTTGG GAGTGGGGCTTTATTTGCACTGAAAATGCAGCTGGTAGATACTCTGAGTCTGGGGGCAGGGCGTTAGGCA GAAACTTTTACCTAGCTATCTAGTTAATCAAAGGCTGGTTTTGGTATGTTCTAGAATATTACTCTAACTT CTGATGTTTGGAGAGCCCCCAAACCATGATTTTAGGGGAAGAACGTAGCTGCATCACCAGTGAGGGAGTT CCCCAAAGTCAGATCCTTTAGAAAGGTGGTAACCGCTAGACAAATTGTGCCTGCCAAGGCCACCTGCCAG TCTTGTTCTGTCAGACATATGGGTAGTTCCCAAGACCTTCCTAGGGATTGAGACAGGGTCCCTCTAAAGC CAGATATGTATGCCCATCACAGGGATTTCTGTCCTGGGAGGCCAGGTGGGTGTTGTAGGACAACTTGATA GGCTGCCATGGTTCTTTGTGGTCTTCCCCTGGGAACCTCAGCAGACAGGGGTCCTGAGCAGTAGAGTCCA TTGGCTCTGAAAATGGCCTGTTTCCTTATTTACTTGACTTGGCACCTTGGAGGGCACGATGGGGCTTACC ACAGCCCAGCCCAGCCCAGCCTCTCAAGTCCAGGGATGGTATTCTGACTGGAGGGGACAGTCAGATGGCT TCAGGAAGTTTCTAAGTTTCACATGTGACACAAACTTGCAACTACTGCCCTCTGGTGGTTAGGTTTGGTT CATTGAGGGAAAGAATGGTTTGGTAAATTGATGGCATCTCCTTGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30431573 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30431573 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7552 same embryo
	EMAGE:7555 same embryo
	EMAGE:7553 same embryo
	EurExpress:euxassay_004595 same experiment
	MGI:4828263 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source