EMAGE mouse gene expression spatial database

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EMAGE:7555

Zim1 zinc finger, imprinted 1 ( MGI:1341879)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004601. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004601_01	euxassay_004601_02	euxassay_004601_03	euxassay_004601_04

euxassay_004601_05	euxassay_004601_06	euxassay_004601_07	euxassay_004601_08	euxassay_004601_09

euxassay_004601_10	euxassay_004601_11	euxassay_004601_12	euxassay_004601_13	euxassay_004601_14

euxassay_004601_15	euxassay_004601_16	euxassay_004601_17	euxassay_004601_18	euxassay_004601_19

euxassay_004601_20	euxassay_004601_21	euxassay_004601_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7555_wholemount_strong.wlz

7555_wholemount_moderate.wlz

7555_wholemount_weak.wlz

7555_wholemount_possible.wlz

7555_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7555_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 07

forelimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 07

forelimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 06 07

hand mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05 06 08 21 22

hindlimb digit 1 metatarsal

moderate

regional

moderate expression: see section 05 06 22

hindlimb digit 2 metatarsal

moderate

regional

moderate expression: see section 04 05 06 07 08 22

hindlimb digit 2 phalanx

moderate

regional

moderate expression: see section 07 22

hindlimb digit 3 metatarsal

moderate

regional

moderate expression: see section 04 05 06 07 08 22

hindlimb digit 3 phalanx

moderate

regional

moderate expression: see section 07 22

hindlimb digit 4 metatarsal

moderate

regional

moderate expression: see section 03 05 06 07 22

hindlimb digit 4 phalanx

moderate

regional

moderate expression: see section 07 22

hindlimb digit 5 metatarsal

moderate

regional

moderate expression: see section 22

submandibular gland primordium

strong

regional

strong expression: see section 08 09 moderate expression: see section 07 10 16 17 18 19

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 12 13 14 15 16 17

telencephalon mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 17 18 19 20 21

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 09 11 12 13 15

rest of cerebellum mantle layer

strong

regional

strong expression: see section 17 18 19 20 moderate expression: see section 03 04 05 06 07 16 not examined expression: see section 10

pons mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 13 14 15

pons ventricular layer

moderate

regional

moderate expression: see section 11

midbrain mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 12 13 15 16 17

midbrain ventricular layer

moderate

regional

moderate expression: see section 11

ventral grey horn

moderate

regional

moderate expression: see section 07 08 09 10 11 12 14 weak expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7606
Entity Detected:	Zim1, zinc finger, imprinted 1 ( MGI:1341879)
Sequence:	sense strand is shown >T7606 GCCGCAGGCTGCGGTGCCTGGAGGCGAGTGCGGACGGACGGATAACCAAGCGCGAGGACGAGAGGTCTTT GGACAACTCACAACAGCCTGTTTTTTCCTACTATGTGGGTCCCAGCGATGGAACTCGAGTTATAAGGCTT GGCAGCAATCGCCTTTACCAGCTGAACCAGCTAGCTGGTCCCAGATTAAGTTTGTTCCCTGTGCCTACAA ATAGGATCATCACAAATGCCGATAACTCCAGCTCCAGGGCATCCGACATCACCATATTTCATTCTCTCAC CATGAAGAGGAGTGTCTCAGAGGTGAAGACTAAAGCCCCAGTTAATGAAGCCTCACCTTCACCAAGTCCT GATCAGACAGCAAACCAGCTCTCTGAAAAGAAGCGAAGAATTCCGATCACGTCGTTGCCAACCTGGTACA AGGAACCAGTGATCTTCAAAGATGTAGCTGTGTACTTCAGCCAGAAAGAGTGGCAGCTACTGGAGCCTGC TCAGAAGGACCTGTACAAGGATGTGATGCTGGAGAACTACGAGAACCTGATCTCTGTGGAATATTATATT TTCAAGCCAAAGTTGATCACCAGGTTGGAGCAAGGAGTTGACCTTTTTGCCAAAGAGAACGATGTTCCTG GAGACCCCCAGCAAGGTGAGGCAGGAGTCAGCAGAAGTGACACCTCTGCAGGGAAGAAGACAGGGGATAA TTCCACAAAGGCAGAAATCCGGAAGCCAGACAATTCTAAGACAACATCACTGGAGAAGCAGAAGGCGGCA GATCAAGGCCGTGGTTCCCAGAGTTTGAGAGCAGAAAAGACCTCAAAAAGTGATGACAGGCCATCTCAAA ATAAGGAGAAATGTGCCTCAACTTCGACTACAGAAGCAAGCAAAACCAGCATACCGGGCAATAAGGAGAA TGAATCTGCAATTCCAGGCACATCTTCGGGTCAAACATCAGCAGCAACTACAACACCACAGAGCGCTCCT TCGGAAAAGCCGACCTCGGGGAAAGATGTTGAGGGAAAACCACAGACCATGAGGAGCAGTTCCACAAATC CAAAGCGCAAGCCTGCCCGACAAGGTAAAAACCACTTTAAATGCAAGGAATGCGGGAAAACCTTCAACCA GACCCTCCACCTCGTGGAACATGAGCGAATTCACACAGGAGAGAAACCCCACAAGTGTGACACGTGCGGC AAATCTTTCCGGCACCTCTCATACTTCCTCACACACTACCGAATTCACACGGGAGTGAGACCCTGCAAGT GTAAGGAGTGTGGCAAAGCCTTCAACAGCAGCTCCACTCTCAACAATCACTGCAGGATCCACTCAGGGGA GAAACCCTTTAAATGTGACGAGTGCGGGAAAACCTTCAAGCAGAGCACAAAGCTCACCCGTCACCAGCGG ATTCATACCGGGGAGAAGCCCTACAAGTGTGGAGAGTGTAACAAGTGCTTCGGCCGCAGCTCATCCCTGA GGGAGCACAAGAGGATCCATACCGGAGAGAAGCCGTACTGCTGTCAGGTGTGTGGGAAGACCTTCAGGGT CAACTCACATCTTTCGGAGCATCAGCGGCTCCATTTAAAGGTGAAGCCGTACAAGTGTGACAAGTGCGGA AAACACTTCCGAAACAGCTCGTACTTGACGGAGCACAAGCAGATCCATGTGCCTGGGGCCCGAGTGGACT GCCCGGAGTGTGGGAAAGTCTTCGCTTGCAAAGTAGCCCTTCTCAAGCACCAGAAGAGACACGAGGCAAA CTCCAGGTACCGGTGCAAGGGGTGTGGAAAGACTTTCAGATGTAAGTCAAGCATCCAAAGGCATGAGAGG CTGCACGCTGGGGAGAAACCTTTCGTGTGCCACAAGTGTGACAAAGGTTTCACTGACAAAACTACATTGA ATAATCACTTGAAAATCCACTCTGGAGATAGGCCAGATCCATGCGCCCAGTGCGGGAGAACCTTTAAAAA ACTGGCTACCCTGCTGATTCACCAGAAAAAGCATATTAAAAAGAAGCCCACAGATGTATAAAAGACCTTT AAAAGCCAGAACTCACATTTGGGTCTTTGGGACAAAGTCAAAACAAGAGAAACGAGACACACATGAACAG TTCCTTCATTTCTTAGTAGCTGTTTTTCAGTAAGCCAGCAGTTTTCTAGAAATGACTGTTTTTGTAACCC TTCCCCTACTTGGAGCATTTGTGCAATATAAACAAACATTTTTGTTGTTGTTGTTACTATTCCAACAGAC AGGTACTACTATCAACTGCATCTGTGGATAGAGGCAAAGGATGACGTCAAACATCCTACAGTGTACTAAA CAGACTCCCTTGCCTTCCAATAACTAAGGAAGATCTGGTTGGCATGCAAATTTGAGGAATTCAGGTGGAC TTTTCTCTGCCAGCCACTACAGTAACCACTAATGAGGCATGGCCGTTTAAATATCAACGTTAAATAGCAA CGTGTGGTAAACACTTAAATGTAGACACTTGGATGAATCATTGACATTCCCAGTCTTCATTGGCTAGTGA GCCTCACTACAGCTAACATTTTCAACACCATGGAAGATTCTGTTTAGAACTGATGTGAATGACCAATAAT CCCTTCATGGGGTGACTCCAAGGAAAATAGGACACAGCTTTGTGAAACTGAATCTATCTGTGCTTTTTTA AAAAAATTTTTTTAACAGCATAATGTAGCATTTTGTAAGGTTTGTGAATGTGCTGGAGCCTGTGATGTTT GGAGCACTTTCAGAACTCCGACTGGCCAGTGCCCACCCGCAGGACTCCTACCCCTACGTTCGGTGGGTTA AAATAATGTCTTTGATAACTGTGAGCATTTGTTGATCGCTATCTACCTATCAAGCATGGTGCTGAACTCT TTATGTGAATTATCAGAGCTAATTTTTACCATATTCCTATGAGATAAGAACTGTTATGTACCTAATTTTC TGTTGAGAAAATCAGGGCTTTTCTTTTAGTATGTTCTCCTCCTCCTCCTTCTTTTCCTTCTTCTCCTTTC TCTTCTTCTTTGATTATTCCTATTCTTATTCTTCCCAAAGGACTCCTACCTTTATTGTGCTTTGGGCAGA ATGATCAGTAGGTAACTAACAGTATCACTGATGCATTTGAGTGTTTATTGGTGTCTTTCTACCTATCAAA TATGGTGCCAAAATCATTACATGAACTATATCAGTTAATGAATTCTACCAATCATATGAAATAGGTATTA TGTACCTAATTTTCATTTTATTTCCTTTTATTTCCCTTTACTTTTTGAGGCAGAGTCTCACTGTGTAGCC CAGACTAGCCTCAAACTTGTGACATTCTCCTGCCCCGGCTTTTTCAGAGCTGGGATGACAGGTGTGAGCC ACCATGCCCAGCTGAGCTGCTTTGTAACGATGGGTTATTTACAAGTTTGCACTTAAAGACTACATTGCTA TTTTTTATGTTGCACTCTTTCAAATAGATAAGATTTATTATATGTTAGCATAAAATATGTTTTTAAATGT GAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30430385 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30430385 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7552 same embryo
	EMAGE:7554 same embryo
	EMAGE:7553 same embryo
	EurExpress:euxassay_004601 same experiment
	MGI:4829356 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source