EMAGE mouse gene expression spatial database

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EMAGE:7557

Lrsam1 leucine rich repeat and sterile alpha motif containing 1 ( MGI:2684789)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004622. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004622_01	euxassay_004622_02	euxassay_004622_03	euxassay_004622_04

euxassay_004622_05	euxassay_004622_06	euxassay_004622_07	euxassay_004622_08	euxassay_004622_09

euxassay_004622_10	euxassay_004622_11	euxassay_004622_12	euxassay_004622_13	euxassay_004622_14

euxassay_004622_15	euxassay_004622_16	euxassay_004622_17	euxassay_004622_18	euxassay_004622_19

euxassay_004622_20	euxassay_004622_21	euxassay_004622_22	euxassay_004622_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7557_wholemount_strong.wlz

7557_wholemount_moderate.wlz

7557_wholemount_weak.wlz

7557_wholemount_possible.wlz

7557_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7557_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 15 16

pons mantle layer

moderate

regional

moderate expression: see section 07 17

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 18 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 05 06 07 08 09 17 18 19 20 21 22 moderate expression: see section 03 weak expression: see section 04

vagus x ganglion

strong

regional

strong expression: see section 08

not examined

regional

not examined expression: see section 11

dorsal root ganglion

strong

regional

strong expression: see section 07 08 15 16 17 moderate expression: see section 06 09 12 13 weak expression: see section 14

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 11 12 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7686
Entity Detected:	Lrsam1, leucine rich repeat and sterile alpha motif containing 1 ( MGI:2684789)
Sequence:	sense strand is shown >T7686 TCGAGACTTTGTCATCCTGAGTTGCGTGTCTTTCTGAAATTTAAAGTTTCGGTGCTCACTTCTATGTTTG AAGGAGACCGGACACCAGCTCAGCTTTTGGGGGCCAATGGTTTGTATCTGTGGCCAAGTCTTCGGAGTGA CTGGCCTACCTTGAGGTCCACCCAAGAATCGGAACATCGGTGGAGGACCTCCCCATCCACAGAGCCAGGG TCCAGAAGAGCTCACACCGGAGGATGCCCCTCTTCTTTCGGAAGCGGAAACCCAGTGAGGAGGCTCGAAA ACGCCTGGAGTACCAGATGTGTCTGGCAAAAGAAGCTGGGGCAGATGACATTCTCGACATCTCTAAATGT GAGCTCTCTGAGATTCCATTTGGGGCTTTTGCAACGTGCAAAGTTCTACAGAAAAAGGTGTTGATTGTCC ATACAAACCACCTCACCTCCCTGCTTCCCAAGTCCTGCAGCCTCTTGAGCCTTGTCACCATCAAGGTTCT GGATCTCCATGAGAACCAGCTGACAGCCCTTCCTGATGACATGGGGCAGCTGACAGTCCTGCAGGTATTG AATGTGGAAAGAAATCAACTCACGCATCTCCCTCGCTCTATTGGGAACCTGCTGCAGCTCCAGACGCTCA ATGTAAAAGACAACAAGCTGAAGGAGCTTCCTGACACCCTGGGGGAGCTGCGGAGCCTGCGGACACTCGA CATTAGTGAGAACGAGATTCAGAGACTTCCCCAGATGCTGGCGCACGTGCGGACCCTGGAGACGCTGAGC CTCAACGCCTTGGCAATGGTCTACCCCCCACCAGAGGTGTGTGGCGCTGGCACTGCGGCCGTGCAGCAGT TCCTCTGCAAAGAGTCAGGACTGGACTATTACCCACCTTCTCAGTACCTGCTGCCAGTCCTGGAGCAAGA TGGAGCAGAGAACACCCAAGACAGCCCCGATGGACCCGCAAGCCGATTCTCCAGGGAGGAGGCTGAATGG CAGAATCGGTTCTCCGACTACGAGAAGCGGAAGGAGCAGAAGATGCTGGAGAAGCTGGAGTTCGAGCGGC GCCTGGACCTTGGGCAGCGGGAGCACGCTGAGCTACTGCAGCAGAGCCACAGCCACAAGGACGAGATCCT GCAGACGGTCAAGCAGGAGCAGACACGGCTAGAGCAGGACCTGAGCGAGCGCCAGCGCTGTCTGGATGCA GAGCGGCAGCAGCTGCAGGAGCAGCTCAAGCAGACGGAGCAGAGCATCGCCAGCCGCATTCAGAGACTCC TGCAGGACAACCAGAGGCAAAAGAAGAGTTCTGAGATTCTGAAATCGCTGGAGAATGAGAGAATAAGAAT GGAGCAGTTGATGTCCATCACCCAGGAGGAGACAGAGAACCTCAGGCAGCGTGAGATCGCCGCCGCCATG CAGCAGATGCTGACGGAGAGCTGTAAGAGCCGGCTCATCCAGATGGCCTATGAGTCTCAGAGGCAGAGCC TGGCGCAGCAGGCCTGTTCCAGCATGGCTGAAATGGACAAGCGGTTCCAGCAGATTCTGTCTTGGCAGCA GATGGATCAGAACAAAGCCATCAGCCAGATCCTTCAGGAGAGTGTAATGCAGAAGGCTGCCTTCGAGGCT CTCCAGGTGAAGAAGGACCTGATGCATCGGCAGATCAGGAACCAGATTAGGCTAATAGAAACTGAGTTAC TGCAGCTGACACAGCTGGAGTTAAAGAGGAAGTCCCTGGACACAGAGACGCTTCAGGAGATGGTCTCAGA GCAGCGCTGGGCACTCAGCAACCTGCTCCAGCAGCTCCTGAAAGAGAAGAAGCAGCGGGAAGAGGAACTC CATGGCATCCTGGCGGAATTAGAGGCCAAGAGCGAAACGAAGCAGGAAAATTACTGGCTCATCCAGTACC AACGGCTTTTAAACCAGAAGCCTTTGTCCTTGAAACTGCAGGAAGAAGGCATGGAGCGACGGCTGGTGGC CCTGCTGGTGGAGCTTTCTGCAGAGCACTACCTGCCCCTCTTCGCCCACCACCGCATCTCACTGGACATG CTGAGCCGAATGAGCCCCGGAGATCTGGCTAAGGTGGGCGTCTCAGAAGCAGGCCTGCAACATGAGATCC TACGGAGAGCCCAGGACCTGCTAGCTGTGCCTAGGGTCCAGCCAGAGTTGAAACCACTCGAGAATGAGGT CCTTGGTGCCCTCGAGCCCCCCACAGCTCCCCGGGAGCTCCAAGAGTCAGTGCGACCGTCCGCCCCCCCA GCTGAACTGGACATGCCGACCTCAGAGTGTGTTGTGTGCCTGGAACGTGAAGCCCAGATGGTCTTCCTCA CCTGTGGCCACGTCTGCTGCTGTCAGCAGTGCTGCCAGCCTCTGCGCACCTGCCCACTGTGCCGCCAGGA GATCTCCCAGCGCCTCCGGATCTACCACAGCAGCTGAAGGCACAGCACCTACCAGCCCTGGCCCTTCCTT GGTACCATCTCCACCACTGCTGGACTCCCACCTACCCTCATTCCCCAGACTAGGGCAAGACTGCCATAGG CCTTTGTCACCCACCACCTGCCACCCCGACCCCCACTGCTCTGAGCACTAGGCAGGCAGGTGGAATGTTC CTGGCCTGTGACATAGCTATTCTGAACAGTGAACAAAAGACAGGGCAAATCTGCTGGTGGTGGAGCCTTG GGGCAGGGGGCCAAGGTCCAGCGTAGGGAGGTGGCCCTGCAGGACTCAGGAATCCATGTTAGTAGCCAAG CCTCAGCGCTACACCCAGCTGTCTGCCCCTCCTGGAGGTGTCCATATCTGTGGAATGTATCAGCCATCCC GTCCTAAGGTGCACCAGTGTCTCTGGGAAGGGGTGGGTTGTGCAAGACTCCCCAGCACCCTTTTGCTTCT TTCTGAACACCCACCCTCCCATATCTGCATCCTTAGCCTACACAGGCAGGTTGATTCAAAACAGGAGCAC CAGTAAATCTGACTCCTTGCAGAGTCACCCCTAGGTCCATGAGAACTTGGGAGGGGTAGGAGTAGGCAGA GTCCGGGGAGAGGGCAGCTCTTCTGGGGAAAAGGGCGAGAGCTGCTGCCCCTGGGTCAGAGGGAAGGCAA CAGAAACATTGCCCCATGGAGAGTTAGGCAACACCCTCTTGAAAGAGGGCGAGGGGAACAGAGCCAGAGA TGGGGGTCTCATTATTTATTTATAAGTAGCCTGTGCTCATGGGACAGGTTGTGTTCCTGCAATGATTATC CTAGGTGAGGGGGCTGCTGAGGCAACTGGAAGGCATGTTAACTTTCAGAGGGGCAGCCCACCCTCACCAG CTGTAGTGAAGGGTTCCATGTGACATGGCACCCTACTGCCCTCCCCAGTATAGTGTATATGGCCTACAGC ATGTGCTCAACAAATATTACTACTTGACTGGCTCTGGGGGAACTAGACATTAGCACCTAGCTCAGGCCTG GAGGCCACCCTCATGGTGGACACATGGGACTGATGAGCCATCAGAAGGCTAGTCACCTGAAAGCTCTATG GCCTCTCCCCTTCTTTCTGAGCCTCAGTTTCTCCCTCTAAAATAGGGGATTGTAAGAATGAGTTAGAGAA AGAGGCCTCAGTACATTAGTTGTCATTTGGAAGTCTGGCTCCCCAGATCTCACAGAGCCAGGCCAGTACA TGTAATCAAAGTACTGAGGAAGCTGAAGCCGGCAGACCCTGGGACTCACCAGTCAGCCAAGCTAACCGAG TTAGTGAGTTCCAGGCTAATGAGACCTCATCTCAAAACACGAGGCGGAATGATCCGAGATCCTTTGGCTT CCACATGCACATGTGAATTTGTACCTGTACACACGTGCAGGAACACACTCAGATCAGGGGCCTGGTAGCT CATATGATGAGCCCAAGAGATCTGGGATGAATGGATCCCTAGATACGGTAGGCCCAGATCTCCCCCACCC CAGCAGCACATGAGGAAATGAGCTTTAAATAAAGCGTTTATTGATGAGCAGAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:6306042 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6306042 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7556 same embryo
	EMAGE:7558 same embryo
	EMAGE:7559 same embryo
	EurExpress:euxassay_004622 same experiment
	MGI:4826011 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source