EMAGE mouse gene expression spatial database

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EMAGE:7559

Dpp6 dipeptidylpeptidase 6 ( MGI:94921)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004610. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004610_01	euxassay_004610_02	euxassay_004610_03	euxassay_004610_04

euxassay_004610_05	euxassay_004610_06	euxassay_004610_07	euxassay_004610_08	euxassay_004610_09

euxassay_004610_10	euxassay_004610_11	euxassay_004610_12	euxassay_004610_13	euxassay_004610_14

euxassay_004610_15	euxassay_004610_16	euxassay_004610_17	euxassay_004610_18	euxassay_004610_19

euxassay_004610_20	euxassay_004610_21	euxassay_004610_22	euxassay_004610_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7559_wholemount_strong.wlz

7559_wholemount_moderate.wlz

7559_wholemount_weak.wlz

7559_wholemount_possible.wlz

7559_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7559_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cervical intervertebral disc

strong

regional

strong expression: see section 11 12 13

lumbar intervertebral disc

strong

regional

strong expression: see section 08 09 10 11 12 13

thoracic intervertebral disc

strong

regional

strong expression: see section 10 11 12 13

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 08 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 17 18

trigeminal v nerve

strong

regional

strong expression: see section 09 17 moderate expression: see section 10

spinal cord

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 14 15

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 11 12 13 14 15 16 17

not examined

regional

not examined expression: see section 01 02 03 04 23

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 23

nasal cavity olfactory epithelium

moderate

single cell

moderate expression: see section 11 12 13 15 16 17 18

kidney pelvis

strong

regional

strong expression: see section 06 07 15

sacral vertebral cartilage condensation

strong

regional

strong expression: see section 08 09 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7687
Entity Detected:	Dpp6, dipeptidylpeptidase 6 ( MGI:94921)
Sequence:	sense strand is shown >T7687 AGCGTCTTCTTCTGCCGAGCCTGTCTTTGCTCTTTGCATTTAAAGAGCAAACTAGGCTGCTCTACCCAGC TCCCTCCCCCCACCGCCCCCCAAAAACCTGGTGATTTTGGAAGTATGGACTAGAATCGCTTTCCTCCTCA CCCTACCATTCTGATCCTGGTGCCTCCAAAGTCTCAGTCAAACCCCTTCTGTCTCTGGGGTGGCGAGTGC CCGGACAGACCATGACCACAGCCAAGGAGCCCAGCGCCTCAGGAAAATCTGTACAGCAGCAGGATCAGGA GCTGGTGGGGAGTAATCCTCCACAGAGGAACTGGAAAGGAATCGCCATTGCGCTGCTCGTCATCCTTGTC ATCTGCTCTCTGATTGTCACCTCAGTCATCCTGTTGACCCCAGCGGAAGATACCAGTCTGTCTCAGAAGA AAAAAGTGACTGTGGAAGATCTCTTCAGTGAAGACTTCAAAATCCATGACCCAGAGGCAAAGTGGATAAG CAATAAGGAATTCATCTACAGAGAACGGAAAGGGAGTGTCATACTGCGGAATGTTGAAACAAATAATTCC ACGGTGTTAATAGAAGGCAAAAAAATTGAATCCTTGAGAGCCATCAGATATGAAATATCCCCAGATAAAG AGTATGTGCTGTTCTCCTATAATGTGGAGCCGGTATACCAACACTCCCACACTGGATATTATGTTCTGAG CAAAATTCCTCATGGGGACCCTCAGAGTCTGGACCCACCAGAAGTCAGCAACGCAAAGCTTCAGTATGCA GGGTGGGGCCCAAAAGGCCAGCAGCTGATATTTATCTTTGAGAACAACATCTACTACTGTGCACACGTTG GAAAGCAGGCAATTCGAGTGGTCTCTACAGGGAAGGAGGGCGTGATCTACAATGGCCTCAGTGACTGGCT GTATGAAGAGGAGATTCTGAAGAGCCACATCGCTCATTGGTGGTCACCAGATGGTACAAGGCTCGCCTAT GCCACCATCAACGACTCCCGGGTTCCACTTATGGAGCTGCCAACGTACACAGGCTCTGTCTACCCCACTG TGAAGCCCTACCACTATCCTAAGGCTGGCAGTGAAAACCCCAGCATCTCGCTCCATGTCATCGGCTTGAA TGGACCCACCCACGACCTGGAGATGATGCCACCTGATGACCCGCGCATGAGGGAATATTACATCACCATG GTGAAATGGGCCACCAGCACCAAGGTCGCTGTGACCTGGCTGAACCGGGCCCAGAATGTGTCAATCCTGA CCCTCTGTGATGCCACCACGGGTGTCTGCACTAAGAAACATGAAGATGAAAGCGAAGCCTGGCTCCACAG ACAGAATGAAGAGCCTGTGTTCTCCAAAGATGGCAGGAAGTTTTTCTTTGTCAGAGCGATCCCACAGGGA GGCCGGGGTAAATTCTACCATATCACCGTATCATCATCCCAGCCCAACAGCAGCAATGACAACATCCAGT CTATCACCTCTGGAGACTGGGATGTGACCAAGATCCTGTCCTATGATGAGAAGCGGAATAAGATCTATTT CCTGAGCACAGAAGACCTGCCACGGAGACGACATCTCTACAGTGCCAACACGGTAGATGACTTCAACAGG CAGTGTCTGTCATGTGACTTGGTGGAGAACTGCACCTATGTCAGCGCTTCTTTCAGCCATAACATGGACT TCTTCCTGCTCAAGTGTGAAGGCCCTGGCGTACCCACTGTTACTGTGCATAACACCACGGACAAGAGAAG AATGTTTGACTTGGAAGCAAATGAGGAAGTTCAGAAGGCTATCAACGACCGGCAGATGCCGAAGATTGAA TATCGAAAAATTGAAGTAGAAGATTACAGCTTGCCAATGCAAATCTTGAAGCCGGCCACCTTCACCGACA CAGCTCACTACCCCTTATTGCTCGTGGTGGATGGTACCCCAGGGAGTCAGAGCGTGACAGAGAGGTTTGA GGTGACCTGGGAAACAGTGTTGGTGAGCAGCCATGGAGCCGTGGTGGTCAAGTGTGACGGCCGAGGCAGC GGCTTCCAGGGAACCAAGCTCCTACAGGAAGTACGGAGGCGGCTAGGCTTCCTGGAGGAGAAGGACCAGA TGGAAGCTGTAAGAACTATGCTGAAGGAGCAGTATATTGACAAGACTCGGGTAGCTGTGTTTGGGAAGGA TTATGGTGGGTACCTGAGCACTTACATCCTCCCAGCCAAGGGAGAAAATCAAGGTCAGACTTTCACCTGC GGCTCTGCGCTCTCTCCAATAACAGACTTCAAACTCTATGCCTCTGCATTTTCTGAGAGGTACCTTGGCC TCCATGGACTCGACAACAGAGCGTATGAGATGACCAAGCTGGCACACCGAGTGTCAGCACTGGAGGACCA GCAGTTCCTGATCATCCACGCCACGGCCGATGAAAAAATCCATTTCCAGCACACAGCTGAGCTCATCACG CAGCTGATCAAGGGAAAGGCCAATTACAGCTTACAGATCTACCCAGATGAGAGTCACTACTTCCACAGCG TGGCGCTCAAGCAGCACCTGTCCAGGTCCATCATCGGCTTCTTTGTGGAATGTTTCAGAGTCCAAGATAA GCTACCAACAGCCACAGCAAAAGAGGAGGAGGAGGAGGACTAACCACGCTTGAGACCGAAGCACAGACAT GGCTCTTTCTACAACAATATGCAACTGAGGAATCCCCACCACCACCACCCTCCCTCCAAGGGCAGTGGGA GTGGAGGTGCGGGCATTCCATAGCATGTGTCTCCATATTGAAGGCCATTTGGTGGTGGGCACCAAGCTCC AGGTTGAAACCAGTCACCACACCGCCTGGAACAACCCCACCTCAGCAGCATGGCCTACTCCTGGCCCCCA GTGACTGGCACATCCCATGTCCCTCCCCCAAGGGACAAAGCAAAGGACAGATGACAGTAAGGGCATACTT TGAACCCAAGAAAACAGGCCCTTAAGTTCAAGCTTTCTCTCTGTAACCTTGAGCCCCACCGTTTGCACTC ACGCACCGCGTTCCTGTTCCTACCTGTGAGTCCTGTCTGGCTTCCTGTCTGACAGAGCTGCACCATGGGA ACCAGACAGCATGGTTGTCTCCGCAGTGCCCAGACATGCCAGGCCTCTCTCTGTTGTTAGAAAAGCCTCC ACTGTCCCTAACTGCACCCAGAAAACAGGTCTTTTATGTACAAAGTAAGACTATAGCTCTGCCCCTGGTT AACCTTGTAGAAATGCTTTGTGTTTTTCTATGGTTTAACTGGACACCCCCCTGTCTCCCACCCTGGACTG TCTCACTCTGAGTTTCTCTCTGTCTCTGTTGCTTTACTTACCTGTTGCTTGTTTGGATAAAGAGATTATT CTGAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6306267 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6306267 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7556 same embryo
	EMAGE:7558 same embryo
	EMAGE:7557 same embryo
	EurExpress:euxassay_004610 same experiment
	MGI:4824382 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source