EMAGE mouse gene expression spatial database

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EMAGE:7609

Incenp inner centromere protein ( MGI:1313288)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004695. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004695_01	euxassay_004695_02	euxassay_004695_03	euxassay_004695_04

euxassay_004695_05	euxassay_004695_06	euxassay_004695_07	euxassay_004695_08	euxassay_004695_09

euxassay_004695_10	euxassay_004695_11	euxassay_004695_12	euxassay_004695_13	euxassay_004695_14

euxassay_004695_15	euxassay_004695_16	euxassay_004695_17	euxassay_004695_18	euxassay_004695_19

euxassay_004695_20	euxassay_004695_21	euxassay_004695_22	euxassay_004695_23	euxassay_004695_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7609_wholemount_strong.wlz

7609_wholemount_moderate.wlz

7609_wholemount_weak.wlz

7609_wholemount_possible.wlz

7609_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7609_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 09 10 11 15 16

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 02 03 04

midbrain ventricular layer

strong

regional

strong expression: see section 08 09 10 14 15 16 moderate expression: see section 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8682
Entity Detected:	Incenp, inner centromere protein ( MGI:1313288)
Sequence:	sense strand is shown >T8682 CCGGTAGGTCCTCGCCTGCGGCGAGCTCGCGGTACTGGGAGTCCAGCCGGTCCGGCGTGGCGGGAAGCTT AATCTGCGGAAGCCGCGGACTGGAAAAGGAGTGGTCCGGGAAGACGCGGCGCCGCCGGACAGTGCCACCA TGGGGACCACAGCCCCAGGGCCTATTTGCCTTCTGGATCTATGTGACCAGAAACTCCTGGACTTTGTCTG CAATGTGGACAACAAGGACTTCATGTGGCTCAAGGAGATTGAGGAGGAGGCTGAGCGCATGTTTATCAGA GAATTCAGCAATGAGCCAGAGCTGATGCCCAAAACTCCTTCTCAGAAAAACCGTCGGAAGAAGAGGCGGG TGTCTAACATTCAGGATGAAAACAGAGATCCCGTGAGGAAAAGGTTATCCCGCAGAAAGTCTCGGAGCAG TCAGGTGGGTACCCGGCACCTCCGCAGCAAGCCCGTTACTATTGTTGAGGAGAATGGCTTCCCTGTGTTG CAGCGGATAACCCGGGCCACAGCTGCAGCAGCGGCAGCAGCAGCGGCAGCGTCTGTGGCATCAGCTTCTT CCTCTTCCACTGCTGGGTCTCCCACAGTGCTGACCAAGAAAGCAGTGGTGGAGATAAGCACTAGTGAACG ACTGAGTGCAGAACTACAACTCACTAAGCTCAAAGGTAGCCTTCCTCCATCTCCAGTTTCCCAAGGCACC CTGACATCAGAGGAGGAACTAACACCCAAGAAATCAGAGGCTGGAAAGCTGGACTCTGTCACAGTGAACT CCCTCAAGGCTACCCCTCAGAGCCCCAAGAACCGAGGGGTTGGGGAAGGACGTTCTGTTTCCAAGCTCAA GATTGCTCGGGCCTCCTGGGGGCTGCAGGACTCTCCTGGCTCTACAGACTCACCATGGCAAGAGCGGGTG CTTTCTCCTATCCTGCTGAATAACATCTTGCCCACAACAGCCAAGAGCCCTCTTGGGAATATTCGGTCAG TGCGGCGAAGCCTGATTTCCCAGGATTCCCAAGTGCCACTAGCCTCGAAGTATAATTTGGTGGCCAAACA AGAAAATGGCAGTCGCAGATCAAGCAGAAGGATTGCCAAGAAGGCTGGCAAAGAGCCAGAGGCCTCTGCT CGAATCATTTGTCACAGTTACCTGGAGAGGCTCCTGAATGTTGAAGTGCCTCAGAATGTTGGCCTGGAGC AGGAGCCTGTCGAGGTGGCCGAACCTGAGGAGGCAGAAGAGGAGCAAGAGGTCTCCAAGAACAGTGGGTG TCCCTCCAAACCCCGCAGTGCCACCAAGATTGCGATTAGTACGCCCACCTCGAAGCCTGCGGCCGCTGGT CAGACAACAACTGTTGAAGAGCAAGAAGCTGAGCTTGACCAAATAGATGGACACAGAGAACCACCCCAGA GTGTCAGGAGGAAACGCAGTTACAAACAGGCAATAAGTGAACCGGATGAGGAGCAACTGGAAGATGAAGA ACTGCAGCCCTGCCAGAATAAGACCCCTTCCCCACCCTGTCCAGCCAACAAGGTGGTACGGCCCCTCCGG ACTTTCCTGCACACAGTGCAGAAGAACCAGATGCTCATGACCCCCACACTGGCCTCCCGCAGCAGTGTCA TGAAGTCCTTTATTAAACGCAACACTCCGCTTCGTGTGGACCCTAAGGAGAAGGAGCGCCAGCGCCTAGA GAGCCTGCGACGGAAGGAGGAGGCCGAGCAGCGGCGCAGACAGAAGGTAGAGGAAGACAAGCGGCGGCGA CTGGAGGAGGTGAAGCTGAAGCGTGAAGAGCGCCTCCGCAAGGTGTTGCAGGCCCGTGAGAGGGTGGAAC AGATGAAGGAGGAAAAGAAGAAGCAGATTGAGCAGAAGTTTGCTCAGATTGATGAGAAGACAGAAAAGGC TAAGGAGGAGCGTCTGGCAGAGAAGGCAAAGAAGAAGGCAACTGCCAAGAAGATGGAAGAGGTAGAGGCA CGAAGGAAGCAGGAGGAGGAGGCACGCAGGCTCAGGTGGCTCCAACAGGAGGAAGAGGAGCGGCGGCATC AAGAAATGCTACAACGGAAGAAGGAGGAAGAACAGGAGCGCCGCAAAGCAGCTGAGGCCAGGCGCCTGGC AGAACAGCGGGAGCAAGAGAGGCGAAGGGAGCAGGAGCGGCGGGAGCAGGAGCGGAGGGAACAGGAGCGG AGGGAACAGGAACGGAAGGAGCAGGAGCGGCGGGAGCAGGAGCAGGAGCGTCTTCGGGCCAAGAGGGAGA TGCAGGAGAGAGAGAAAGCCCTGCGACTCCAAAAGGAACGACTTCAGAAGGAACTGGAGGAGAAGAAGAG GAAGGAAGAGCAACAGCGCCTGGCTGAGCAGCAACTGCAGGAGGAGCAGGCAAAGAAAGCTAAGGAGGTG GCAGCAGCCAGGAAAGTCCTGAACATGACTGTGGATGTGCAGTCTCCTGTTTGTACCTCATATCAAATGA CTCCACAAGGACCCAAATCCATCCCCAAGATCAGCGTAGACGATTATGGGATGGACCTAAATAGTGATGA CTCCACAGATGATGAGTCCCACCCCCGGAAACCCATCCCTTCCTGGGCCAAAGGCACCCAACTCAGCCAG GCCATTGTCCACCAGTACTACCACCCTCCAAACATTCTAGAGCTCTTCGGATCTATTCTCCCACTGGACT TGGAGGACATCTTTAAGAAGAGGAAGACTCGCTACCACAAGCGCACTAGCTCTGCTGTTTGGAACTCACC ACCCCTGAAAGCCACCATGGTCCCCAGCAGTGGGGACTAGACTATCTAGCCTGAAGAGTCCATGAGGCAG GCCCTCAGTCATTTTGGAGCACAGGGCCCATGTGTGTCTTTCTGTTTGTTTCTCTGTCTGTTTTGATGTT GGGCTGGTGCACTTCTGCCTGATATGCCATTGTTCAGGTGGCTATCAGTGTCCTCTGTTTGTCAGGTATT CCCAGACCTGTTGGGAGGATGGGCTTCATGCCTGGGGAAGAACCAGGTTGAGCACTGATGGCTCTGTAGG CAGTACTCTGTAAATAAATAGATTGTTACAGTTCAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6401188 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6401188 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7611 same embryo
	EMAGE:7608 same embryo
	EMAGE:7610 same embryo
	EMAGE:7612 same embryo
	EMAGE:7607 same embryo
	EurExpress:euxassay_004695 same experiment
	MGI:4825589 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source