EMAGE mouse gene expression spatial database

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EMAGE:7612

Fignl1 fidgetin-like 1 ( MGI:1890648)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004690. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004690_01	euxassay_004690_02	euxassay_004690_03	euxassay_004690_04

euxassay_004690_05	euxassay_004690_06	euxassay_004690_07	euxassay_004690_08	euxassay_004690_09

euxassay_004690_10	euxassay_004690_11	euxassay_004690_12	euxassay_004690_13	euxassay_004690_14

euxassay_004690_15	euxassay_004690_16	euxassay_004690_17	euxassay_004690_18	euxassay_004690_19

euxassay_004690_20	euxassay_004690_21	euxassay_004690_22	euxassay_004690_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7612_wholemount_strong.wlz

7612_wholemount_moderate.wlz

7612_wholemount_weak.wlz

7612_wholemount_possible.wlz

7612_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7612_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 11 12 weak expression: see section 13

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 weak expression: see section 13

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 09 10 15 16

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 moderate expression: see section 02 03 22 23

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 moderate expression: see section 12 13 14 15 16 weak expression: see section 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8685
Entity Detected:	Fignl1, fidgetin-like 1 ( MGI:1890648)
Sequence:	sense strand is shown >T8685 CGGTCCCGGCAGGGAGCACAGCTCATCCTGTGCTGATAACATCGAGAAGTGTTCAGTGCCTGGTAAAGTA CATAGACCTTGCTTCACTTGGAACTCGGCCTTGATTTCTGCCGTTGGTCATAATCAGCAGAGTTCTCTCT AAACCTTTGACATGGAGACGTCCAGCTCCATGTCTGTGGAGACGACTAGGTCTGTGCAGGTGGACGAATG GCAGAAGAATTACTGTGTGGTTACATCCAGCATATGTACACCAAAGCAGAAGGCCGATGCATACCGTGCA CTACTACTGCATATTCAGTATGCATATGCCAACTCCGAGATCTCTCAGGTCTTTGCTACCAACCTGTTCA AAAGGTATACAGAAAAATACTCTGCAATTATTGATTCTGACAATGTTGTAACTGGCTTGAATAACTATGC AGAGAGCATTTTTGCTTTGGCAGGATCTCGACAGGCTGACAGTAACAAGTGGCAGTCTGGATTGTCAATA GATAATGTTTTCAAAATGAGTTGTGTACAGGAGATGATGCAGGCTGGCAAGAAATTTGAAGAGTCTCTGT TGGAACCTGCTGATGCATCAGTAGTCCTGTGTAAAGAGCCCACCGCCTTTGAGGTTCCTCAGCTTAGTGT TTGTGGAGGTTCTGAAGACGCTGACATATTATCCAGTTCAGGTCATGACACAGATAAGACCCAAGCCATT CCAGGGAGCAGTCTGAGATGTTCCCCTTTTCAGAGTGCTCGGCTGCCTAAGGAAACTAATACCACTAAGA CATGCCTCACCTCCTCAACATCTTTAGGTGAGTCAGCCACTGCAGCATTTCACATGACACCATTATTTGG AAACACCGAAAAGGACACTCAAAGCTTTCCTAAAACCAGCACAGGACTAAATATGTTCTTATCTAATCTG TCTTGTGTTCCTTCTGGCTGTGAAAACCCTCAAGAAAGGAAGGCTTTTAATGACTCTGACATCATTGACA TACTTTCCAATCCAACACTGAACAAGGCTCCTAGTAAAACAGAAGACAGAGGCCGAAGGGAAGATAATAG CCTGCCTACCTTTAAAACTGCAAAAGAACAATTATGGGTAGATCAAAAGAAAAAGGGCCATCAATCCCAG CATACATCTAAATCTTCTAATGGTGTTATGAAAAAGTCTCTGGGAGCTGGGAGGTCGAGAGGGATATTTG GCAAGTTTGTTCCTCCTGTATCTAATAAGCAAGACGGAAGTGAGCAGCATGCCAAGAAGCACAAGTCTAG TAGGGCAGGGTCTGCAGAACCAGCACACCTCACTGATGATTGTCTGAAGAACGTGGAGCCAAGGATGGTT GAACTTATTATGAATGAAATTATGGACCATGGGCCTCCAGTACATTGGGACGATATTGCTGGAGTAGAAT TTGCCAAAGCCACAATAAAGGAAATCGTTGTGTGGCCCATGATGAGGCCAGATATCTTTACTGGATTGCG AGGGCCCCCTAAAGGAATTCTGCTCTTTGGCCCTCCAGGGACTGGTAAAACTCTGATTGGCAAGTGCATT GCTAGCCAGTCTGGAGCAACATTCTTCAGCATCTCTGCTTCATCGTTGACTTCTAAGTGGGTAGGTGAGG GAGAAAAAATGGTCCGTGCACTGTTTGCTGTTGCCAGGTGTCAGCAGCCAGCTGTCATATTTATTGATGA AATTGATTCTTTATTGTCTCAACGAGGAGATGGTGAACATGAATCTTCAAGAAGGATAAAAACGGAATTT TTAGTTCAGTTAGATGGAGCAACCACATCTTCTGAAGACCGGATTCTTGTGGTGGGAGCTACAAATCGGC CCCAAGAGATTGATGAAGCTGCCCGGAGAAGATTGGTGAAAAGACTTTATATTCCCCTCCCAGAAGCTTC AGCCAGGAAACAGATAGTAGGTAATCTAATGTCTAAGGAGCAATGTTGTCTCAGTGATGAAGAAACTGAT CTGGTAGTGCAGCAGTCTGATGGGTTTTCTGGCGCAGATATGACACAGCTTTGCAGAGAGGCTTCTCTTG GTCCTATTCGCAGTTTGCACGCTGCTGACATTGCTACCATAAGTCCAGATCAAGTTCGACCAATAGCTTA TATTGATTTTGAAAATGCTTTTAAAACTGTGCGACCTACTGTATCTCCAAAAGACTTGGAGCTTTATGAA AACTGGAATGAAACATTTGGTTGTGGAAAGTGAATATAGCGATTGAAAGGAGAAGCTGTTATCTAGTAGT CGTCTTTACCTTTAGCCTCGGAAGCTTGCTGTGCTACTTGTATTGTTTTGGAGTATATCCTGAATTCTGT GCCTCAGATTAGAATGATAACAGCTTGACTACTGACTGATATATTAGTATGTTGTATTTGCCTTCCAGTT ATATAATATATAAACTCATTTCATTTAAATAGAATGACCTTTTGTTTCTAAGTGAATTCAAATTAGAACT TTAGTCTGGTAATAATAAACTTTGTTAGTGTAGTATTATTATTGCTTACATTTGAAACAGCATTGCATTC TTGACTTAGAGTTAAGATCTTACCAAAAGCTAGAGTGAAAGCAACAGGGAGTAAACTACCGTAACCCGTG CTGGCTTGTAACTTGTTTTGTGGGCTTTTTTTCTATTAAAATTAACTAATAAAAACATGTTAGAAATAGC AAGATGTAATCTTGTATCAGGCATGTAGCCTGCCTCAAAAGATAAGTGTGGCTTTTTTCAAGGGATCTGG AAAGTTTTCTATTATTGTTTTGTCTGATAATTCTTAAGAGCCTATTTCAAACTAAATATTTTTAAATTAA AGAGAATAAAAGTTGTTAGTTTGTTACCATTTAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6401467 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6401467 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7611 same embryo
	EMAGE:7608 same embryo
	EMAGE:7610 same embryo
	EMAGE:7609 same embryo
	EMAGE:7607 same embryo
	EurExpress:euxassay_004690 same experiment
	MGI:4824864 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source