EMAGE mouse gene expression spatial database

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EMAGE:7621

Ptpn12 protein tyrosine phosphatase, non-receptor type 12 ( MGI:104673)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004732. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004732_01	euxassay_004732_02	euxassay_004732_03	euxassay_004732_04

euxassay_004732_05	euxassay_004732_06	euxassay_004732_07	euxassay_004732_08	euxassay_004732_09

euxassay_004732_10	euxassay_004732_11	euxassay_004732_12	euxassay_004732_13	euxassay_004732_14

euxassay_004732_15	euxassay_004732_16	euxassay_004732_17	euxassay_004732_18	euxassay_004732_19

euxassay_004732_20	euxassay_004732_21	euxassay_004732_22	euxassay_004732_23	euxassay_004732_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7621_wholemount_strong.wlz

7621_wholemount_moderate.wlz

7621_wholemount_weak.wlz

7621_wholemount_possible.wlz

7621_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7621_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8702
Entity Detected:	Ptpn12, protein tyrosine phosphatase, non-receptor type 12 ( MGI:104673)
Sequence:	sense strand is shown >T8702 CGGAGCGGGCGCTGCGGCGCGCGGGCGGGCGGCGGGGGGGACGCGGGAGGATGGAGCAAGTGGAGATCCT GAGGAGGTTCATCCAGAGGGTCCAGGCCATGAAGAGTCCGGATCACAATGGGGAGGACAACTTCGCCCGG GACTTCATGCGATTGAGAAGATTGTCTACCAAATATAGAACAGAAAAGATTTATCCCACAGCCACTGGAG AAAAAGAAGAAAATGTTAAAAAGAACAGATATAAGGACATACTGCCATTTGATCACAGCCGAGTTAAGTT GACTTTGAAGACTCCATCCCAAGATTCAGATTATATCAATGCAAATTTTATTAAGGGTGTGTATGGGCCA AAAGCATATGTGGCAACCCAAGGGCCTTTGGCGAATACAGTCATAGACTTCTGGAGGATGATATGGGAGT ATAATGTTGTGATCATCGTGATGGCCTGTCGAGAATTTGAGATGGGAAGGAAAAAGTGTGAGCGCTACTG GCCTTTGTATGGAGAAGATCCTATAACATTTGCACCATTTAAAATTTCTTGTGAAAATGAACAAGCAAGA ACCGACTACTTCATCCGAACACTTTTACTTGAATTTCAAAATGAATCCCGTCGGCTCTATCAGTTTCATT ACGTGAACTGGCCAGACCATGATGTTCCTTCGTCATTTGATTCTATTCTGGACATGATAAGCTTAATGAG GAAATACCAAGAACATGAAGATGTGCCTATTTGTATTCATTGCAGTGCAGGCTGTGGACGAACAGGTGCT ATTTGTGCCATAGATTACACGTGGAACTTACTGAAAGCAGGGAAAATTCCAGAGGAATTTAATGTATTTA ATTTAATACAAGAAATGAGAACACAGAGGCACTCGGCAGTACAAACAAAGGAGCAGTATGAACTTGTTCA TAGGGCTATTGCTCAACTGTTTGAAAAACAGCTACAACTGTATGAAATTCATGGAGCACAGAAGATCGCT GATGGTAATGAAATTACCACTGGAACTATGGTCAGTTCCATTGATAGCGAGAAGCAAGACTCTCCTCCGC CAAAGCCACCGCGGACTCGAAGTTGCCTTGTAGAAGGGGATGCCAAGGAAGAAATACTACAGCCACCAGA ACCTCACCCGGTGCCACCCATCCTGACGCCATCACCTCCTTCAGCCTTCCCAACCGTTACCACTGTGTGG CAGGACAGTGACAGGTACCACCCAAAGCCAGTGCTGCACATGGCCTCACCAGAGCAACACCCAGCCGACC TCAACAGAAGCTATGATAAATCAGCGGACCCAATGGGGAAAAGTGAATCAGCTATTGAGCACATAGATAA GAAGTTAGAGCGCAATTTAAGTTTTGAGATTAAGAAAGTCCCTCTCCAAGAAGGGCCCAAAAGTTTTGAT GGGAACACACTCTTGAATAGGGGACATGCGATTAAAATTAAATCTGCTTCATCTTCTGTAGTTGACAGAA CCTCTAAACCACAGGAGTTAAGTGCAGGTGCCCTAAAGGTTGATGATGTATCTCAGAATTCTTGCGCGGA CTGTAGTGCGGCTCATTCACACAGAGCTGCTGAGTCGTCAGAGGAGTCCCAGAGCAACTCACACACACCT CCACGGCCAGACTGCTTGCCTCTCGATAAGAAAGGACACGTAACGTGGTCACTTCATGGACCTGAAAATG CCACACCTGTACCCGACTCACCTGACGGCAAATCCCCAGATAATCATTCTCAGACTCTGAAAACCGTGAG TTCCACACCCAACTCCACCGCAGAAGAGGAAGCCCACGATCTTACAGAGCACCACAACAGCTCCCCTCTG TTGAAAGCTCCCCTCAGCTTTACCAACCCTCTTCACTCTGACGACTCGGACTCAGACGGAGGGAGCTCTG ATGGTGCTGTGACCAGGAACAAAACTAGCATTTCAACAGCAAGTGCCACAGTGTCTCCTGCCAGTAGTGC TGAGAGTGCTTGCACTAGGAGAGTATTGCCGATGTCCATTGCCAGACAGGAAGTAGCAGGCACGCCGCAT TCAGGTGCTGAGAAAGATGCTGATGTTAGTGAGGAGTCGCCTCCTCCTTTACCTGAACGAACTCCTGAGT CTTTTGTATTAGCAGATATGCCTGTAAGACCTGAGTGGCATGAACTTCCAAATCAGGAGTGGTCTGAACA AAGGGAATCTGAAGGCTTGACAACCTCTGGAAATGAAAAACATGATGCAGGGGGCATCCACACAGAGGCT TCTGCAGACTCTCCACCTGCTTTCAGTGACAAGAAAGATCAAATAACAAAAAGTCCAGCAGAAGTCACAG ATATTGGTTTTGGTAATCGCTGTGGAAAACCTAAAGGACCAAGAGAGCCACCTTCAGAATGGACATGATG CAGGGAGTGAAAGGACACTTCACATTATACTGGAACACTCAAGTGCCACTGAAAGCCAGGCTTATAGTAT TCCATCTTTAAATGTGGGACTGATAGCAGTGTAGATTGTTACCTTAATGTTTTTGCTGGGACCATCTACC TGCCTTATACTACACTTAGGAAAAAGTATTATATTGTTTATTTTGTAACTTCAAGTATTATTGCCTTAAT GTCTCTTAACCCTGTTACAAGCTGCTTGTAGACCTGTTAATATAGTAATACTTTTATGATAAATTGAGTT AAAGGACTACTCTTTTGCTGTTTTATCATGTATGCATTATTTTGTATATGTACAGGGCAAGTAGGTATAT AATTTGATAAAGTCCTAAAATATTATTAACAGAAGATGTAAGAATTTCTGCATGGTCTAAGTCTTTGTGT ATCTTACTTGTAAATTATTTGCCCTGAAGTTTTAGAAAACAGTTTCTGAATTTTAAACTTGCTGGATTCA TGCAGCCAGCATTGCAGGTTATCAGAGATCAAAGATTGTAATAATAATACATTTTGTAAATTGTAAGCAA AAAGTTATTTTTATACTATATACTGTCTAATTGTTCATCCTAATGGTTCTTGTTTTCATCTAGTCATGGA GATTCAGTAAGTGCCTTGGAACAATATTGAATTCTCTTAGCTCGTGTGTGTTACTTTAAAATTTGAATTC AACTGGGGTTAGAAGACTATCAGAATATATGTATGTTTCAGGATATTTGACCTGCCATTAAAAAAACAAA CAGTTTTACACTGCTAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6402545 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6402545 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7620 same embryo
	EMAGE:7624 same embryo
	EMAGE:7623 same embryo
	EMAGE:7622 same embryo
	EMAGE:7619 same embryo
	EurExpress:euxassay_004732 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source