EMAGE mouse gene expression spatial database

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EMAGE:7624

Kdm2b lysine (K)-specific demethylase 2B ( MGI:1354737)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004720. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004720_01	euxassay_004720_02	euxassay_004720_03	euxassay_004720_04

euxassay_004720_05	euxassay_004720_06	euxassay_004720_07	euxassay_004720_08	euxassay_004720_09

euxassay_004720_10	euxassay_004720_11	euxassay_004720_12	euxassay_004720_13	euxassay_004720_14

euxassay_004720_15	euxassay_004720_16	euxassay_004720_17	euxassay_004720_18	euxassay_004720_19

euxassay_004720_20	euxassay_004720_21	euxassay_004720_22	euxassay_004720_23	euxassay_004720_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7624_wholemount_strong.wlz

7624_wholemount_moderate.wlz

7624_wholemount_weak.wlz

7624_wholemount_possible.wlz

7624_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7624_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

metanephros

strong

spotted

strong expression: see section 07 08 09 10 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8699
Entity Detected:	Kdm2b, lysine (K)-specific demethylase 2B ( MGI:1354737)
Sequence:	sense strand is shown >T8699 GGCGCGCGGTGGCGGCGGCGGCTCCCTCCTCGCCGCGCGGGAGCCCGAGCGCTAGCCAGGGTGAGCCGAG CCTGCTCCCTGCGGGCGGCGGCGGCGCTGCTCCAGCCATGTCAGCGCGCGCGAGGCTGGGCCCACCATGA GCCATGGCCATGTCCGTGAGCGCCGAGGACGACGACTATGAATCGGAGCCCGACCAGAACCGGGTGGTGG GTCGGCCTAAGGGCAAGTTGGGCCCGGCCTCAGCGGTGAAGTTGGCTGCCAACCGAACAACAGCAGGAGC TCGCAGGCGCCGGACGCGATGCCGCAAGTGCGAGGCCTGCCTGCGGACGGAGTGTGGAGAGTGCCACTTT TGCAAGGACATGAAGAAGTTTGGAGGTCCTGGGCGCATGAAGCAGAGCTGCATCATGCGGCAGTGCATCG CGCCAGTGCTGCCCCACACCGCCGTGTGCCTTGTGTGTGGCGAGGCAGGGAAGGAGGACACAGTGGAAGA GGAAGAAGGCAAGTTTAACCTCATGCTCATGGAATGCTCCATCTGCAACGAGATCATCCACCCTGGATGC CTTAAGATTAAGGAATCGGAGGGTGTGGTCAACGATGAGCTTCCCAACTGCTGGGAGTGTCCGAAGTGTA ACCATGCCGGCAAGACCGGGAAACAAAAGCGTGGCCCTGGCTTTAAGTATGCCTCCAACCTGCCTGGCTC CTTGCTCAAGGAGCAGAAGATGAACCGGGACAACAAGGAAGGGCAAGAGCCTGCCAAGCGGAGAAGTGAG TGTGAAGAGGCTCCCCGTCGGAGGTCAGACGAGCACCCCAAAAAGGTGCCTGCAGATGGCATCCTCCGCC GAAAGTCTGATGATGTGCACCTGAGGAGGAAGCGGAAATACGAGAAGCCCCAAGAGCTGAGTGGACGCAA GCGAGCCTCGTCGCTTCAAACGTCCCCCGGTTCCTCCTCTCACCTCTCGCCGAGGCCCCCTCTAGGCAGC AGTCTCAGCCCTTGGTGGAGATCCAGTCTCACTTACTTCCAGCAGCAGCTAAAACCTGGCAAAGAAGATA AGCTTTTCAGGAAAAAGCGGCGGTCCTGGAAGAACGCTGAGGATCGTCTGTCACTGGCCAACAAGCCCCT TCGGCGCTTTAAGCAGGAACCGGAGGACGATCTGCCTGAGGCACCTCCTAAGACCCGGGAGAGTGATCAG TCACGTTCCAGCTCACCCACTGCTGGTCCCAGCACTGAGGGAGCTGAAGGCCCAGAAGAGAAGAAAAAGG TGAAGATGCGCCGGAAGCGGCGACTTGTTAACAAGGAGCTGAGCAAAGAGCTAAGCAAGGAGCTCAACCA TGAGATCCAAAAGACGGAGAGCACCCTGGCTCACGAGAGCCAGCAGCCCATCAAGTCAGAGCCTGAGAGC GAGAACGACGAGCCCAAGAGGCCCTTAAGCCACTGCGAGCGCCCCCACCGCTTCAGCAAAGGGCTCAACG GCACACCTCGGGAGCTGCGGCACTCGCTGGGACCTGGCCTGCGTAGTCCACCTCGTGTTATGTCCCGGCC CCCGCCCTCTGCATCCCCACCCAAGTGCATCCAGATGGAGCGTCACGTGATCCGGCCACCGCCCATCAGC CCCCCACCTGACTCGCTGCCCCTGGATGATGGAGCAGCCCACGTCATGCATAGGGAGGTGTGGATGGCAG TCTTCAGCTACCTCAGCCACCGAGACCTGTGTGTCTGCATGCGGGTCTGCAGGACCTGGAACCGCTGGTG CTGCGATAAGCGGTTGTGGACCCGCATCGACCTGAACCGCTGCAAGTCCATCACACCCCTGATGCTGAGC GGTATCATCCGGCGACAGCCTGTCTCCCTTGACCTCAGTTGGACCAACATCTCCAAGAAGCAGCTGAGTT GGCTCATCAACCGGTTGCCTGGGCTCCGAGACTTGGTGCTGTCAGGCTGCTCATGGATCGCTGTCTCAGC CCTCTGTAGCTCCAGTTGTCCATTGCTCCGGACCCTGGATGTCCAGTGGGTAGAAGGACTAAAGGATGCC CAGATGAGGGATCTCCTGTCTCCACCCACAGACAACAGGCCAGGTCAGATGGACAATCGGAGCAAGCTCC GGAACATTGTAGAGCTGCGCCTAGCTGGCCTGGACATCACAGATGTCTCCCTGCGGCTCATTATTCGCCA TATGCCCCTGCTCTCGAAGCTCCAACTCAGTTACTGTAACCACATCAATGACCAGTCCATCAACCTGCTC ACTGCCGTCGGCACCACCACCCGAGACTCGCTGACAGAGGTCAACCTATCAGACTGTAATAAGGTAACTG ACCTGTGCCTGTCCTTCTTCAAACGCTGTGGAAATATCTGTCATATTGACCTGAGGTACTGCAAGCAAGT CACCAAGGAAGGCTGTGAGCAATTCATAGCTGAAATGTCTGTGAGTGTCCAATTTGGGCAAGTGGAAGAG AAACTCCTGCAAAAACTAAGTTAGTTCCAGGACAAGTATGTAAATATGGAGGGTTGGGGGGAATACAACC AGGAAGGGTGAAAGACTGCAAAATCAAGTGGGCCTATTATTTCCCTTTGGAATTTGAAACAAGAGACTAC AGGACACAGTTCTGTTTTGCAAATCTTGCCAAGGGGGAAGCCATTCAACCACCCATTTTGGGATGAGTGA GCCAGATGTTTTCCTTTGCTCTTTCTGCATCGTAACTGCACTGCTTTCTGGACCATTTCTAAGGCGGCCT TTAGAAGACATTCCAATTGGAAAGGAAGATGAGCTTTGGAGAAATCCTCTTAGAAGCCTAAGCTTGGTGT TTTTATCAGTAGTTTTTGTTTGGGGCACTTGTTCCTTGCAATGCTGAGGCTTCTGGCGTCAACATGGAGT GGACCACACACACACCATCTTCAGCCGTCGTTGATACATTTTGTTTAGTTGGTTTTGTTACATCTTACAT TATGCAGAACTATTTTTGTATAAATTGTTTAAAAATTATTTATGGGAGATTTGAATGCATACCAGTGTTT TTATTATTTAGATTGCCAACTGTCCTTCAGGTAGGAGAGTTAACCTGTACTATGTCAGCATAAAAAGCCC ATCTCCAGATGTGCCCAGGTGTGACAGGGGGCTATGACCTTCCACTTAAAAAGCATGTTTAACTGGATGC TGAGAGCCTACTGTGTATGTCAAGAAGCTACACAAGCATGTTGTGGATATGTGTAAAGTATAGTCCAGGT AAAACACTTTGCCAAGTTTCAATAGACTTCACCTCTCCCTCAGACTTGAGCCTTTACACTCAGGAGGGTT TGCTCCAAGCATGAGCTCTTGTATTCTACTTACATAGGTCTAATTTATACCAAGAATCAGGTAGAATAAA TGTGCCCACACCCTCTCCTTTGTCCTCCTCCTCTCAAGTGTTCAGTTCTCACTTTAGTTGGTAAGTTCTG CATCCCTTGTTGTTTGGTGGTGTTTCCTAACCTTTCCCTGATGCAGTTTGACCTTTGTTGGATTTATATA AACAATTGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6402440 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6402440 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7620 same embryo
	EMAGE:7623 same embryo
	EMAGE:7622 same embryo
	EMAGE:7621 same embryo
	EMAGE:7619 same embryo
	EurExpress:euxassay_004720 same experiment
	MGI:4825743 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source