EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7625

Eef1d eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) ( MGI:1913906)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004717. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004717_01	euxassay_004717_02	euxassay_004717_03	euxassay_004717_04

euxassay_004717_05	euxassay_004717_06	euxassay_004717_07	euxassay_004717_08	euxassay_004717_09

euxassay_004717_10	euxassay_004717_11	euxassay_004717_12	euxassay_004717_13	euxassay_004717_14

euxassay_004717_15	euxassay_004717_16	euxassay_004717_17	euxassay_004717_18	euxassay_004717_19

euxassay_004717_20	euxassay_004717_21	euxassay_004717_22	euxassay_004717_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7625_wholemount_strong.wlz

7625_wholemount_moderate.wlz

7625_wholemount_weak.wlz

7625_wholemount_possible.wlz

7625_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7625_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8710
Entity Detected:	Eef1d, eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) ( MGI:1913906)
Sequence:	sense strand is shown >T8710 TGAGCTCGCAGTTCCAGGTTTTGGTTGCTGCGTCCTTGGCTGGCGTGAGGCCAAAGCAAAAATGAGGAGT GGGAAGGCCTCTTGTGCACTGGAGACCGTCTGGGAGGACAGGCACAAGTATGAGGGAGCCGAGCGGCGCT TCCACGAGCAGGAGGCCACACAAGTCGTCGCCGCCGCCGCCACTGCCGCCGCCACTGCCTCCGCCTCTGT CCAGCAGCTCCTGGACGAAGTGCCAGCTGTGAACGGGCCCAGCCAGGAGGACACAGAGGACACTGAGGAG GCAGAGGCCCCAAACACCAGCAGCAGGAGTGATCCCGGGAAGAGCCACGAGTGCAAGAAGCCGATACAGA AAAAGAGGAAGCGCTCCCCGAAAAGTTGGCTGGGCCAGGCAGACCTGGCCCTTGTGGGCCTCTCGGCTGA CCACGTATGGTTAGACAAGCCACTCTTCGACCAGGCAGAAAGCTCCTACCGTCAGAGGCTGGCAGATGTA GCTGCCCAGGCAGCCCAGCCGCCTGCTCTGGCCCCTCGGGGGCCCTGTACACATGGAAGCCATGTGGCAT GCCACCATGTAACCTGGGGGATCTGGGTCAACAAGTCTTGCTTTGATCAAGCCGAGCGGGCTTTTGTGGA GTGGTCTCAGTCCTTGCTGCTGGCTGCAGAGGGGAGCCATAGGCAGGGGACTCCTGACACAGGCCAGCAG GCTGTCACTCCTGACCTGGCCTTGGCCTGCCAGCCTTGCCCACCAGCCAATGGCCAGCCTCCTCTGGGCA GCCTGCAGGCACTGGTGCGGGAGGTATGGCTGGAGAAGCCCCGGTACGATGCCGCTGAGAGGGGCTTCTA TGAGGCCCTGTTTGATGGTCATCCCCCAGGGAAAGTGCGCCTGCAGGAGCGAGCCAGTCAGGCAGAGGGT ACCCGGAGGGGCCGCAGAGACCATCGGAGCTGCAATAACGTAGGAAACAAGAGAGCTGGGTCAAAACGGG CCAACGGGGAGGCTCCTCCTGCTTTTCCCTACTGGTACTTCCTACACAAGGACGCAGAGGCCCCCTGGCT CAGCAAGCCTACCTACGACAGCGCTGAGTGCCGCCACCATGCCGTTGAGGCCCTGCGCATAGCCTGGCGC CTAGAAGCTGCCTCCCTGGCTCACCGACCCACACCCCGTTCTGGCCCATCCATGTCCAGCCTGAGACCCA ACAGAAAAATGGCTACAAACTTTCTAGCGCATGAGAAGATCTGGTTTGACAAGTTTAAATATGATGATGC AGAAAGGAGATTCTATGAGCAGATGAACGGGCCTGTGACCTCCGGCTCCCGCCAGGAGAATGGTGCCAGC GTGATCCTCCGAGACATTGCAAGAGCCAGAGAGAACATCCAGAAATCCTTGGCTGGAAGCTCAGGCCCTG GAGCCTCCAGTGGACCTGGTGGAGACCACAGTGAGCTCATTGTGAGGATTACCAGTCTGGAAGTGGAGAA CCAGAACCTTCGAGGCGTGGTGCAAGATTTGCAGCAGGCCATTTCCAAGTTGGAGGCCCGGCTGAGCTCT CTAGAGAAGAGTTCACCTACTCCCCGAGCCACGGCCCCACAGACCCAACATGTCTCTCCTATGCGTCAAG TGGAGCCCCCAACCAAGAAAGGAGCCACACCAGCAGAGGACGATGAGGACAAGGACATTGACCTGTTCGG CAGTGACGAGGAGGAAGAAGATAAGGAGGCTGCCCGACTACGGGAGGAGAGGCTACGCCAGTACGCAGAG AAGAAGGCCAAGAAGCCCACACTGGTGGCCAAATCCTCCATCCTTTTGGATGTTAAACCTTGGGATGATG AGACTGACATGGCCCAGCTAGAGACTTGTGTGCGTTCCATCCAATTGGACGGGCTGGTTTGGGGGGCCTC CAAGCTTGTGCCTGTTGGCTATGGCATCCGGAAGCTGCAGATCCAGTGTGTGGTGGAGGATGACAAAGTG GGCACCGACTTGCTCGAGGAGGAGATCACCAAATTTGAGGAGCATGTGCAGAGTGTCGACATCGCAGCTT TCAACAAGATCTGAATGTCCGAGAGAGTGTGGTTTCATGTGTGTGAGGACCTGCTGAGATTAAAGACTGA GACTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:6403006 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6403006 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7629 same embryo
	EMAGE:7630 same embryo
	EMAGE:7627 same embryo
	EMAGE:7626 same embryo
	EMAGE:7628 same embryo
	EurExpress:euxassay_004717 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source