EMAGE mouse gene expression spatial database

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EMAGE:7628

Vhl von Hippel-Lindau tumor suppressor ( MGI:103223)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004739. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004739_01	euxassay_004739_02	euxassay_004739_03	euxassay_004739_04

euxassay_004739_05	euxassay_004739_06	euxassay_004739_07	euxassay_004739_08	euxassay_004739_09

euxassay_004739_10	euxassay_004739_11	euxassay_004739_12	euxassay_004739_13	euxassay_004739_14

euxassay_004739_15	euxassay_004739_16	euxassay_004739_17	euxassay_004739_18	euxassay_004739_19

euxassay_004739_20	euxassay_004739_21	euxassay_004739_22	euxassay_004739_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7628_wholemount_strong.wlz

7628_wholemount_moderate.wlz

7628_wholemount_weak.wlz

7628_wholemount_possible.wlz

7628_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7628_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8708
Entity Detected:	Vhl, von Hippel-Lindau tumor suppressor ( MGI:103223)
Sequence:	sense strand is shown >T8708 GCCTTCAAGTTGCCCGGAGCGCGTCTGGGACCGGCAGACCTGTCCCGGAGCGCGTAGATCCGCACGCACG CACGCGCCGCCCACGTCCAGCTTGCGAATCCGAGGGACCCGTTCCAATAATGCCCCGGAAGGCAGCCAGT CCAGAGGAGGCGGCGGGGGAGCCCGGTCCTGAGGAGATGGAGGCTGGGCGGCCGCGGCCGGTGCTGCGCT CGGTGAACTCGCGCGAGCCCTCTCAGGTCATCTTCTGCAACCGCAGCCCGCGCGTCGTGCTGCCTTTGTG GCTCAACTTCGACGGCGAGCCTCAGCCCTACCCGATCTTACCACCGGGCACCGGCCGCCGCATCCACAGC TACCGAGGTCATCTTTGGCTCTTCAGGGATGCGGGGACCCATGATGGACTTCTGGTTAACCAAACGGAGC TGTTTGTGCCATCCCTCAATGTCGATGGACAGCCTATTTTTGCCAACATCACATTGCCAGTGTATACCCT GAAAGAGCGGTGCCTTCAGGTTGTGCGGAGCCTGGTCAAGCCTGAGAACTACAGGAGACTGGACATCGTC AGGTCACTCTATGAGGATTTGGAGGACTACCCAAGTGTGCGGAAGGACATACAGCGACTGAGCCAAGAGC ACCTTGAGAGTCAGCACCTGGAAGAGGAGCCTTGAAGGAGTCCATGGAGATTAAGTGTTCCTGAGTTTCA GCCTTGATGGTCCGAGATTGATCTACACATAGGACAGGTCACTTTCTTTCAGTTTTAAAATGGTTCATTC TTGGAGTAAAACTATCCATCACGTAAAAGAAAGTTAACTAACATCCCTGGGCTTTGTAGTGTTTAAGAAT AAACATGCAAAGTGCCACTGCGTCTGCCCTTTGTAGAGCACTCACCCGAGGGAGGAAGACGTTTTCAGTT TTGCTTCCTGGTGAGGCTGGAAGTTGAGTGTAAGGATGACTGTGTAATAAGTTCAGCAGCAGGAGTTGTA CTGTGTCCTTTCATTTGAGCAGAGGGCTCTTGCTTGGGAAGGCAGAGAAGGCCCTTCCCAAACGCTGGGA CAGACCTCCTGTGGAGGCCCGCTGCCTAAAGCGTGGAGTCTTCAGTCATGAATGTTGGACGAAGAAACCA TTGGATGCTTGGTGGGAGAGTTGGGAGGGCCTGGCTCTTTGTCTGAGGAGAGCCTTAAGTGTTCATGTAA GGAACAGCTTAGCAGCTTCTGATTTCCCACGTCCCAGCACTTTCTCTCTGTTTTTTATTTTATTTTTGAG ACTTTTGTATAGCCCAGGCCAGCCTCAGTCACTCAGCAGAGGATTCCTGACCCTCTGGGATTATGTCTCC TGACATTGGGAACACATGTGTGCACACAGCCTGCTCTGCTCTTCCTTTTCCCATGTTGAGACGGGCTCCT GCTGTAGTCCTGCCTGGCCTGAGCTGGAGTCGGTCCTGCCTCCTGACCGACCGTCTGGCTGCTTTCTAAG CTGGGTAGACTTCCCATACTCACTGTTACAGACAGTTACAGCAGAGAAGAACCATCCTTGTGTAAGTCTG GTTTTGAGAGCTCCTGTCTCTGAGGAAGCTGTTGGCAGGAGCAGGGATTTCTGTTCCTTCTTGTCAGCCC CTAGCATTCCACTTTGTTTTGGCAGGCGCTGGTTCTGCTTTGTAGGTGGAAGTGAAGCTCCACTCAGCCA CAGACTCCAGACAGTGGGTCACAGAGCAGTCTGGAATGTGTAGCAGTCTTATGGATGGTCACAGCTTTTC TTACATATTCATATTCTATCAAACGTTTTGTAAAGTATAATTTTTTATTTATTTTTAATCCCCTTAGAGA AAAAGTAAAAGTAGAGAAGCCTGGGTATTGTGTACACAACAGAAAGGACAGTCAAGGAAAGGGAAGCAGA AAGGCTCTTATGTACTCTCAGCTTCATGCAACCCCTGTAACTGGCCGCATATGTAGGGCATATTTTAATT GGTTAAAGCAGTTTGTTGTGGCTGAGGTCATAAGATATATACAGTTTTGTATCCTGATTTTTCACTGAAC TTCATTGTTTTTTGTTTGCTTGCTTTTCCCTTCTTCTTTGTTTTTTGCTTTATTGAGATAGGGTTTCTCT GTAGCCCAGGCTGTCCTAGAACTCCCTTTGTAGACCAAACATCTGATTACTGGCCTCACATGGAGAGATC TGTCTCTGCTTTTCCAGCGCTGGGATTGAAGTAGGCCTAATCTTCATCTAACTTCTTAGCTTGTCTTTTC CATGTTACTACACACACTGTGTAAATACCAGTGTGCTACCCTCCTTCTTTGAGGGTGACTGTTGGGAGTA TTTGTCCAAATCCTGGGCTCCTTGAGTGCAGACTGATGCTCTAAGATCAGCAAAATGACTGAACTACATC CTAACACTTCCCAGTGTCCTCTCAGTGTCTCTTCTTATCTGCAGCATTGCAGGAGTGAAGTGGCCACAGA CCAGTGTGGAGGCCTGTGTGTGGGAAGTCAGCAGGTGCCAAGCTATTGAAACATTGGTTTTATTTTTTTA AACCGATCTCTGTCTCTAAAAGATGTGATGTTTGTGTTATTCAGCTGGAAGTTTGAATACTGTTCTCTGT GCTGTCCAACAAGTGAAGAGTTATGAAATGAGCCACTTGTCCTGGGACATCCTGTAATGAGTTCCTCGGG CTTTTCTCCTGTGAGTTTTCTGAAAAAAGCTGACCTTTTTAGTACAATAAACGGTGCTAATTGAAGGAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6402536 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6402536 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7625 same embryo
	EMAGE:7629 same embryo
	EMAGE:7630 same embryo
	EMAGE:7627 same embryo
	EMAGE:7626 same embryo
	EurExpress:euxassay_004739 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source