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EMAGE:7707

Foxj3 forkhead box J3 ( MGI:2443432)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7707
euxassay_007440_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7707Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7707_wholemount_moderate.wlz
7707_wholemount_weak.wlz
7707_wholemount_possible.wlz
7707_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7707_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7789
Entity Detected:Foxj3, forkhead box J3 ( MGI:2443432)
Sequence:sense strand is shown

>T7789
GGCTGCTCCCGCCGCCGCTGCCGCCGCCGCCGCCACTCTCGGCGCCGCTCCGGGCCTGGGCCGCTCTCTG
AGGGCGCCGTTGGGACCTTTCGCGGGGAACGGAGACGCACGCAGCGGCCGGAGGCTCGGCCGGGCCGTCC
CGGCCTGCGTAGCCTGAGATTCTCTTTGTGGCCAGATGGGTTTGTATGGACAAGCTTGTCCATCTGTAAC
TTCATTAAGGATGACATCTGAACTGGAGAGCAGCCTAACATCTATGGACTGGTTACCCCAGCTCACCATG
AGAGCAGCCATCCAGAAATCTGATGCTACACAAAATGCACATGGAACGGGAATTTCTAAGAAGAATGCAC
TCCTTGACCCAAATACAACACTGGACCAGGAAGAAGTCCAACAGCACAAAGATGGAAAACCTCCGTACAG
CTATGCCAGCCTCATCACATTTGCAATTAATAGCTCACCTAAAAAGAAGATGACATTAAGTGAGATTTAC
CAATGGATTTGTGATAACTTTCCGTATTATAGAGAAGCTGGCAGTGGTTGGAAGAATTCTATACGACATA
ATCTGTCACTGAACAAATGTTTCCTTAAAGTGCCTCGGTCCAAGGATGATCCCGGAAAGGGTTCTTACTG
GGCAATAGACACCAATCCGAAGGAAGACACGCTGCCTACTCGGCCAAAGAAGAGGGCAAGATCCGTAGAA
CGGGCCTCAACTCCATATAGCATAGATTCAGATTCTTTGGGAATGGAGTGTATTATTTCGGGAAGTGCCT
CTCCAACTCTGGCAATCAACACTGTGACTAACAAAGTAACATTGTACAACGCTGACCAGGATGGTAGTGA
TAGCCCTCGAAGCAGCCTTAACAACAGTCTCTCAGACCAGAGTTTGGCGTCTGTTAATTTGAACAGTGTT
GGAAGTGTACATAGTTATACACCGGTCACTAACCATCCAGAACCAGTCTCTCAGCCATTAACACCTCAGC
AGCAGCAGCAGCCACAGTACAACCTTCCAGAACGAGAAAAACAACTCCTTTTCACAGAATACAATTTTGA
AGATCTCAGTGCCTCATTTCGGAGTCTTTATAAGTCAGTTTTTGAGCAGTCACTTAGTCAACAAGGTTTA
ATGAGCATCCCTTCGGAATCTTCCCAACAGTCCCACACTTCATGTTCCTATCAACACTCTCCTAGCAGTA
CAGTGACCTCTCACCCACACAGCAACCAAAGCAGCCTGCCCAACAATCACAGTGGCCTCAGTGCCACAGG
TAGTAATTCGGTTGCACAGGTCTCGCTGTCCCACCCCCAGATGCACCCCCAGCCCTCTCCACATACACCC
CATAGACCGCATGGTTTACCCCAGCACCCACAGCGTCCCCAGCACCCAGCACCACACCCACAGCAACACA
GCCAGCTACAGCCGCCTCACTCCCAGCACCCCCCTCCACATCAGCACATACAACACCATCCGAACCATCA
GCATCAGACATTAGCACATCAGCCACCGCCACCCCCGCAACAGGTATCCTGTAATTCTGGTGTTTCAAGT
GATTGGTATGCAACACTCGATATGCTGAAGGAAAGCTGTCGAATTGCCAGCAGTGTTAACTGGTCAGATG
TAGACCTTTCACAGTTCCAAGGTCTGATGGAGAGTATGAGGCAGGCGGATCTCAAGAACTGGTCATTAGA
TCAGGTTCAGTTCGCTGATCTCTGTTCCTCTCTTAATCAGTTCTTCACACAGACTGGCCTTATCCACTCG
CAGAGTAATGTTCCGCAGAATGTCTGTCACGGTGCCATGCACCCAGCAAAGCCTTCCCAACACATCGGGG
CAGGAAATTTGTACATAGATTCCAGGCAAAGTCTCCCTCCCTCAGTGATGCCACCCCCTGGTTATCCTCA
TATTCCACAGGCGCTCAACACTCCAGGAACTACGATGGCAGGTCATCACGGAGCCATGAACCAGCAGCAC
ATGATGCCTTCCCAAGCCTTCCCGATGCGGCGCCCTCTGCCTCCAGATGACATCCAGGATGACTTTGATT
GGGATTCAATAGTGTAGGCTTGTTTCTGCAAGACAGCAGACTCCAGGATTCTTTTTGTTCAGTGAAAGGA
CGGATTAGAGGCCGGTTGAGAAAACAAAGCTATGTTACCAGTGCTATCTAAATTCCTTTCGAAGACAATC
AGAGATTCTAGCTGGGTAACTTACTGCTTTTATCCAACCCAAGCAAGAACTACATGTTTGTCTCCCTGCC
AGTACCCTCTGTAGCTCCTAACTGTTGTGATTTGGACAGCTTTTTGCATATTTGTGTCAGTTTGATGTTG
ACCACAAGTGCCAGACTGATTTTTCAGACAGAGCCTATTTTGCTGCGAGCAGTTTATAGATACATATGTG
TAAATATGTGTACAAATATTACTGAAAGGCTTCAAGTTTTTTCTAATTGGATTATTGTATCTTGAAAAGA
AAGTTACTGTCAGTTTTTATTCCTTGTTAGGCCATTTTCTGCAGGATGCTTTCAAATGATACAGAGAGCT
GAAAGGAAATCAGTTTTTCAAAGCCCAGTTTCCTTTATTTAATCTTCAGAAATAGGAGTAATCATGAATC
CACCGAGTAAGTCAAAGAATCTGGAGTTGGTACTCAAAAACAGTGCAGAGGGCCGTTGTTCCTGAGCAAG
CTTGTCACTTGTCATTCGGTGTTGTAGAGAGAGTGTTGTACCTGTCAGTGTGATTCCTCAGCTGCATCTT
GGCAGCTCTGAAATGTGTGGGCTGACTGAAACTGGGTATTGCTTGGCTCTTCAAACAAACCAATGTCTCC
ATAGTCACTTATGTATTTATTATTCAAAAGTGTTATTTTAATATTTATTGCTCTGTGAATGCACAGTTCT
GTTGGATTCACTAGGGAGGGATTTGTCTGAAAGCATGGTTATTTTTCTGGATAAAGTTTACAGACAAAGA
CAATCAGAAGCATTGGTCCTTCTCTTTTATCATCACTCCTTACCAGGTGAGCGTGCTTCCTGTCTTGCTT
AGCCTTCCTGCTGGGGGAGACTTAATAGCTACATAGGAATTTCCTGGAATTTGAATCAGTCTATTTAGGC
AGCAAGATACAGAGGCTGGGTTTATACAGCAGTGTCCCTGCTGTTGGTAACGCTAGCAGGGGCTGCATGC
AGCTCCAGAGTCTTATCTGCTTCAGTCTTGAGTGCAGTGACTGACTGCGTGACCTGCTGTTGGGTCTGTG
TGTCCCCAACATGAAGCACCTCAGAGCTTTGATCAGTTGCTTTGTTTTGTGTCATGTCACAGCTAGCATG
GGTTGAATGTGTTGATTTTATAATGAAAGTAAAGAAAGGAGATTCTCCTCCCCACAGTGAATAGATCGTT
AATTGGTTCTTTACAGAGAGGGAAATCCATTTTAATGAGAAAATAGATTCTGTCAAATCAAGAAGAAATA
ACTGGTCACCAGTTGCTTGTTAGTGTCTCTGGGCTAAGCAGTGCTTAATCACATTGACTAACCTCAGCGA
GTCTGTGGATGAGTAATCTCTACTGGAACAAAGGTTTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
TGTGTGTGTGTGTGTGTAAGTGCAAGAGATCATGAAAGAGACGGGGCACGGGGAGAGAATGAGAATATCT
GTGTATGTGTTAATGGAGTGTTGTCTTGAATGAATCCCTGGGAAGCCATGGGGAGTGCAGGGAAGAAGGG
AGAGCCTCGGGCTTCGCTGTTGCTTGGACCCCCACTTGGCATGAGAGAAAGAAGCTCAGGTACAGAGGAT
TCTCAACCAATAGCCAACAGGCCTCACTCGGAAGGACTGGGCAAGATGAGATGGCTGTCTGCAGAGAAAA
AGCTTGGGAAAATGAGGTCCTCAGTAGTTTGCTCAAGTCTCTGTTTTCTAGACACCAAAATGGCTGCTCT
GTGACTAGGATTGCTCCGCAGCAGTGTGCACTTTAAATGATTGGGTGTTGGAGTATCGCAGTCTGAAACA
GCATGGCTTGATGAATTTACAGATTATGTGAAGCTAACTTCCCATTTGACAATTACTGATTTGCAGTGAT
CGATATATTTATGAATGTTTAAACTGACCAAGAATGGCACTTGAGGCCACTCACCACTCTTCTGTGATTC
CCTGTTTCCCTGGGGCTCAGCAAGCCTGAGTGCCTGGGCAGGGAAGGCTACCTGGTGGGCGCAGAGTCCC
TCTAGTGGCCATTTCTGATTTATTTGATGACACACAGCAAGTTCTCTAGGGCTGAAATTAACTGGAGACT
TGGGGTAAGGACTAACAAACATACATACACAGATGGGGCGCTCTGTCCAGGAGAAGAGTCCGACTGGCAT
TTGTGGCAGTGTTTGAAATGTAAATGTACTCCTGTGTGTTCCTGTAAATATTTAATATGTGTCTCAGACG
TCCTTTGAAGTGGGAGGGGATCGATCTTGGGATAATTTCAAATGGAATAGAGTATTTTGATATGTTCATT
GAAAGGGTGATGTGTACATACTTATATTGTATATATGTGGTGACATGCATTGGCTTTTGTGCAGAAACAA
CCTGCTCTTGTGCTGCTGTTGGACAGTCAAGTGTTTTAATGTTTCTACAGTTTTCCTATTGCACGATTTC
ATATTCTGCCAATGGTGAGTGGCACCCATTCTTTGTAACCGTATAGTGCTGTAAAGAAATATTCCAAGTG
TCATTAGGATTGTCGCTGCCAGAATTGATATGCATGGATGGCACTTAAAATAAATATATTATGATAACTG
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6314981 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6314981 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7708 same embryo
 EMAGE:7710 same embryo
 EMAGE:7709 same embryo
 EMAGE:7706 same embryo
 EurExpress:euxassay_007440 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS