EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7708

Lrrc61 leucine rich repeat containing 61 ( MGI:2652848)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007437. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007437_01	euxassay_007437_02	euxassay_007437_03	euxassay_007437_04

euxassay_007437_05	euxassay_007437_06	euxassay_007437_07	euxassay_007437_08	euxassay_007437_09

euxassay_007437_10	euxassay_007437_11	euxassay_007437_12	euxassay_007437_13	euxassay_007437_14

euxassay_007437_15	euxassay_007437_16	euxassay_007437_17	euxassay_007437_18	euxassay_007437_19

euxassay_007437_20	euxassay_007437_21	euxassay_007437_22	euxassay_007437_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7708_wholemount_strong.wlz

7708_wholemount_moderate.wlz

7708_wholemount_weak.wlz

7708_wholemount_possible.wlz

7708_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7708_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6930
Entity Detected:	Lrrc61, leucine rich repeat containing 61 ( MGI:2652848)
Sequence:	sense strand is shown >T6930 GCTTTAACCGGTAACAATCGCGGCCGGTGAGTTATCACGGGGCCTGTCCCATCTCTGTCCGCTCCATGTT GTCAGCCGTATCTAGGCAGCCACCCTCCACTCCTAGAGCTCATCATTCCTGAAGATCTGTTCCAAAAGAA CTTCACACTCAGTTGATGTGCACAGACCTTTGGCCCAAGCAGAATTGAGGGAGAGGAGCCAGCTGATCCT CAGAGGCACCTGCATCCCTGTGACTGACAGCACTGCCAGGGCCCAGCGGTCTGAACTGTCTTCAGTATCA TGGAGCCTCCTGGAGAGAAGCCAGGAGAGGCTGAGGCGCTGAGCATCACACCCCAGCTGCTGAAGTCCCA CTCAGGAGAATTTGCCCTGGATTCCATCCTGTTGCTGAAGCTTCGGGGTTTAGGGGTGGTGGACCTGGGT TGTTTGGGGGAGTGCCTGAACCTTGAGTGGCTTGACCTATCAGGCAATGCACTTACCCACCTGGGCCCAC TGGCATCCCTGCGCCAGCTGGCTGTGCTCAACGTCTCCAATAATCGGCTTACAGGGCTGGAGCCACTAGC AGCTTGTGAGAACCTGCAGAGCCTCAATGCTGCTGGTAACCTGCTGACCACTCCTGGTCAGCTGCAGTGT CTGGCTGGGCTGCAGGCCCTGGAGCACCTGAGGCTCCGGGACCCTTTGGCCCGGCTCAGCAACCCGCTCT GTGCAAATGCTTCCTACTGGGCTGTGGTCCGAGAGCTACTGCCTGGCCTCAAAGTCATAGATGGGGAACG AGTGAGTGGGCGGGGCAGTGAGCTGTACCAGTTATGCCGGGACCTGGACAGTTCCTTGCGCTCCGGCTCC AGCCCAGGGCCCAGAGCCATCGAGGCCCAGCCATGGGTAGAGCCCGGCTACTGGGAGTCCTGGCCCATCC GGAGCAGCTCCATTCTGGAGGAGGCCTGCCGACAGTTCCAGGACACACTGCAGGAATGCCTTGACCTGGA TCGTCAGGCCAGCGATAGCTTGGCCCAGGCCCAGCAGGCTCTCAGCCCTGCAGAAACCACCTCTTCCTTT GTCTTCTGAATGCAACTCATAGCCAAGGTGGTCTGATCGTAGACATTCCTTCCCTGCCCCATGCGCTTGC CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTCTATTTATATCTGGTCACTGACCCTGCAGA CTAGGTTGTTCATTCATCCGTTTCTTGAGAGCTCCTAACTCCTGCATCTCCTCAAGGGTGTTTCCTCCCT CCTCCCACAGCCTCAAGGACACACTTTCCAGATCGCCTCTAGAAGGGAGAGGAGATCCCGTCACTATCTG GCAAATGCACCATGCGGCCAGGCTGCAGGGGCTCTGGGCATGCTGTGCCTGAGCTCCCTCAAATGCCCAA CCTAAGGCTTTGTGGGTAAGGTTACTTGCCACCTGCTTACTCTTATGAAATAGTTCAGTGCTCATATTAT CACTTGTCTCGTCTCCAGCAAATACGCGGGGCTTCTGAACCTCTCTGCTTTGGTCCTGTGGAAGGCTCCC ACACTTGCCCTACCTGCTGCTTCCTGGTACTAAGCAGGAAGTGTGGACACACACACACACACACACACAC ACACACACACACACACACGTCTTTTTGAGCTTCCCTTATATGTACAGGTTGAGCTGAGGGAGAACAACAC AAGGTTGGAAAGGAACAGACTCAGCAATTTTATTAGCCTGGAGTTGAAAGTCTCTGGGAGACGATATGCT CCTGCTGGGTGCTGCCACAGCTGTACAGGGAGTAAGGTGAAGTCCTAGAGAAGAAGGGAAAACCAGGTTG GGGGTGCGGGAGACTGTTTGGGTCAGATTCTAGCTTTTGTCACGTTTCACAAGTCCTTTCTCAACCCAAT TTCCATGCCAAGGGCTGGCTCTCCCCATTAACCACTTACCTGTCTAGGGCTTATTTGGTTCTCAGGGCCC TGGAGAAGGCAAACTGTCCAGGTAGGAAGTAGCCGTGGGGGTCTTCGCCAGCCTGTGCTATCTTAATGCA TTGCAACTCCTGAGGATCCTGCTAGAACCAGTGTTTGCTGCAGCAGTTCTAGGAAGGGAGGCACCGCCAC AACCCCCAGGGGAATGTCCCGAGGTGGGGGTGGGGGGACCCAGCTCTTCTTTTCAGGTTTCCTGTTCTTC TCCCATAGACCCAAATTTGATCTGTCCCTCCAGGCTTCTCCCTAACCTCGTTTCCTTCTTACCTGAGGCC CTTGCTTCAGAATTGGGCAGTGGACTATCCGGCCTAGAATTTTACCCTTGGGGTCCATTTTAATGCAGGC CAGGCATTTCCGCCTTCTCTGGGGAGGAGAACAGGGATGTCAGAGTGGAGACTAGCCTCTGGCATGCTCT GGAGTGGGCGATGTAGTGATTTGAATAAGAATGTCCCCCATAGGCTGACATATTTGAAGATTTGGTCTCC AGTTGGTAGTGCTGTTTGGGGAGGTTTTGAAGGTTTGACCTTGATGGAGGAAGTACGTCAGTGGGGCAGG CTTTAAGATTTTAAAACCTCGAACCACTCCCAATTCACTCTTTCTGCGTTCTGTTTACGAGGAAGGCTGT GAGCTCTCAGCTTCCTGCTCTGGCCACCATGCCTGCTGGCATCCACATCTCCCTGCCTCCCACCATGATG GACTTTTATTCCTCTGGAACCCTAAGCCAAAATAAACCTTTCCTATTATTAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3981816 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3981816 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7710 same embryo
	EMAGE:7707 same embryo
	EMAGE:7709 same embryo
	EMAGE:7706 same embryo
	EurExpress:euxassay_007437 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source