EMAGE mouse gene expression spatial database

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EMAGE:7709

Hmgn2 high mobility group nucleosomal binding domain 2 ( MGI:96136)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007442. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007442_01	euxassay_007442_02	euxassay_007442_03	euxassay_007442_04

euxassay_007442_05	euxassay_007442_06	euxassay_007442_07	euxassay_007442_08	euxassay_007442_09

euxassay_007442_10	euxassay_007442_11	euxassay_007442_12	euxassay_007442_13	euxassay_007442_14

euxassay_007442_15	euxassay_007442_16	euxassay_007442_17	euxassay_007442_18	euxassay_007442_19

euxassay_007442_20	euxassay_007442_21	euxassay_007442_22	euxassay_007442_23	euxassay_007442_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7709_wholemount_strong.wlz

7709_wholemount_moderate.wlz

7709_wholemount_weak.wlz

7709_wholemount_possible.wlz

7709_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7709_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9078
Entity Detected:	Hmgn2, high mobility group nucleosomal binding domain 2 ( MGI:96136)
Sequence:	sense strand is shown >T9078 GCAGCGAGGACGACCCCCGGACGGAGCAAACCCGCGCGCCGCTGTATCCCCGTGCTCAGCGCCGACGTCC CCTGCCGCCACCATGCCCAAAAGAAAGGCTGAAGGGGATGCTAAAGGAGACAAAACCAAGGTGAAGGACG AGCCACAGAGAAGATCTGCGAGGTTGTCTGCTAAACCTGCTCCTCCAAAGCCAGAGCCCAAACCTAAAAA GGCCCCTGCGAAGAAGGGAGAGAAGGTACCCAAGGGGAAGAAGGGGAAAGCTGACGCCGGGAAGGATGCG AATAATCCTGCAGAAAATGGAGATGCCAAAACAGACCAGGCACAGAAAGCTGAAGGTGCTGGAGATGCCA AGTGATGTGTGTGCATTTTTGATAACTGTGTACTTCTGGTGACTGTACAGTTTGAAATACTATTTTTTAT CAAGTTTTATAAAAATGCAGAATTTCGTTTTACTTTTTTTTTTTAAGCTATATTGTTAGCACACAGAACA CTTCATTACTGGGTGGGGGAAGGATCATGTGTCAGTAACAAAATCTCTCCCAAGCTGGATTGAGGACAGA AAACCTCTTTCCCTGATAATTTTGGAAGGCTCCTGTTGGCTCCCAGGAGAGAGATCCTGGTCTTGACCTA GGTGGCCACCAAGGCACAACAATGCCTTGTGGTCTGGGGAAACTATAAATTCACTTTTATATCCTCTTCC CCCTGTACTATCAACATAGACTTAATTCCCTTAAAAACCAGAGACCTGTTGGAACCGGGCCCCCAAAATT GGTTTCCCAGTCCATTGAGTGATGGGGACTTTGCAGTGACTTCATTGAGTGTTCTCAAAAGAGCACTGGT TCCTTTTATAAAAGATTGTGGATCTTCAGATTGATAATTCTGCCTAAAAGTCAGGGTCGGCTTGTGAAAA GTTGTTAAAACAACATCCTTAATGTGAAATGTCAACCCTCACTCTAAGCTACTTCCCCCTTTTCAAAGCA TTGAATGAAGACTTCATTGGGTTTTATAGTGGCTTTCTGATTTTGGTAGTCTATACAAGAAGGGAATTTG GAAGTTCTTGTATATTGTTGATTGTCTGCCCATGTCCTGCCTGAAATACCATGATTGTTTATGAAAAGAA TCTTTAATAAAGCTGGTTACAGTTAGGCTTGGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6819800 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6819800 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7708 same embryo
	EMAGE:7710 same embryo
	EMAGE:7707 same embryo
	EMAGE:7706 same embryo
	EurExpress:euxassay_007442 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source