EMAGE mouse gene expression spatial database

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EMAGE:7769

Apc adenomatosis polyposis coli ( MGI:88039)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007660. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007660_01	euxassay_007660_02	euxassay_007660_03	euxassay_007660_04

euxassay_007660_05	euxassay_007660_06	euxassay_007660_07	euxassay_007660_08	euxassay_007660_09

euxassay_007660_10	euxassay_007660_11	euxassay_007660_12	euxassay_007660_13	euxassay_007660_14

euxassay_007660_15	euxassay_007660_16	euxassay_007660_17	euxassay_007660_18	euxassay_007660_19

euxassay_007660_20	euxassay_007660_21	euxassay_007660_22	euxassay_007660_23	euxassay_007660_24

euxassay_007660_25

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7769_wholemount_strong.wlz

7769_wholemount_moderate.wlz

7769_wholemount_weak.wlz

7769_wholemount_possible.wlz

7769_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7769_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

hindbrain

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 08 18 19

trigeminal v nerve

moderate

regional

moderate expression: see section 09 16

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16

cervico-thoracic ganglion

weak

regional

weak expression: see section 09 15 16

cervical ganglion

weak

regional

weak expression: see section 08 16 17

thoracic ganglion

weak

regional

weak expression: see section 13

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 12 16 17 18

neural retina

strong

regional

strong expression: see section 01 02 03 22 23 24 25

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35939
Entity Detected:	Apc, adenomatosis polyposis coli ( MGI:88039)
Sequence:	sense strand is shown >T35939 CCTCAAGACTCCAGCCTCTAAAAGCCCCAGTGAAGGGCCGGGAGCTACCACTTCTCCTCGAGGAACTAAG CCAGCAGGAAAGTCAGAGCTTAGCCCTATCACCAGGCAAACTTCCCAAATCAGTGGGTCAAATAAGGGGT CTTCTAGATCAGGATCTAGAGACTCCACTCCCTCAAGACCTACACAGCAACCATTAAGTAGGCCAATGCA GTCTCCAGGGCGAAACTCAATTTCCCCTGGTAGAAATGGAATAAGCCCTCCTAACAAACTGTCTCAGCTG CCCAGAACATCATCTCCCAGTACTGCTTCAACTAAGTCCTCCGGTTCTGGGAAAATGTCATATACATCCC CAGGTAGACAGCTGAGCCAACAAAATCTTACCAAACAAGCAAGTTTATCCAAGAATGCCAGCAGTATCCC CAGAAGTGAGTCGGCATCTAAAGGACTGAATCAGATGAGTAACGGCAATGGGTCAAATAAAAAGGTAGAA CTTTCTAGAATGTCTTCAACTAAATCAAGTGGAAGTGAATCAGACAGCTCAGAAAGGCCTGCATTAGTAC GCCAGTCTACTTTCATCAAAGAAGCCCCAAGCCCAACCCTGAGGAGGAAACTGGAGGAATCTGCCTCATT TGAATCCCTTTCTCCATCTTCTAGACCAGATTCTCCCACCAGGTCGCAGGCACAGACCCCAGTTTTAAGC CCTTCCCTTCCTGATATG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97369. Forward Primer - name:097369_F_cDNA_Apc, sequence:CCTCAAGACTCCAGCCTCTAAA; Reverse Primer - name:097369_N_SP6_cDNA_Apc, sequence:CATATCAGGAAGGGAAGGGCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7770 same embryo
	EMAGE:7773 same embryo
	EMAGE:7774 same embryo
	EMAGE:7772 same embryo
	EMAGE:7771 same embryo
	EurExpress:euxassay_007660 same experiment
	MGI:4823172 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source