EMAGE mouse gene expression spatial database

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EMAGE:7772

Arhgap21 Rho GTPase activating protein 21 ( MGI:1918685)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007662. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007662_01	euxassay_007662_02	euxassay_007662_03	euxassay_007662_04

euxassay_007662_05	euxassay_007662_06	euxassay_007662_07	euxassay_007662_08	euxassay_007662_09

euxassay_007662_10	euxassay_007662_11	euxassay_007662_12	euxassay_007662_13	euxassay_007662_14

euxassay_007662_15	euxassay_007662_16	euxassay_007662_17	euxassay_007662_18	euxassay_007662_19

euxassay_007662_20	euxassay_007662_21	euxassay_007662_22	euxassay_007662_23	euxassay_007662_24

euxassay_007662_25

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7772_wholemount_strong.wlz

7772_wholemount_moderate.wlz

7772_wholemount_weak.wlz

7772_wholemount_possible.wlz

7772_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7772_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 15 16

pons mantle layer

moderate

regional

moderate expression: see section 07 weak expression: see section 17

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 weak expression: see section 20 21

ventral grey horn

moderate

regional

moderate expression: see section 09 10 14 weak expression: see section 13

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 15 16 17 weak expression: see section 06 07

neural retina

moderate

regional

moderate expression: see section 01 02 03 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35943
Entity Detected:	Arhgap21, Rho GTPase activating protein 21 ( MGI:1918685)
Sequence:	sense strand is shown >T35943 AAGAAGTCTGCTCGCTTCAAGTCAGACAGTGGGAGCCCCGGAGACACCAGGACTGAGAAAGAAACTCCTG CCCTGGCCAAGATGTTTGACGTCATGAAGAAAGGCAAGTCGACAGGGAGCCTCCTCACACCCAGCAGAAG TGAATCCGAAAAGCAGGAGGCCACTTGGAAAACAAAAATAGCAGATCGTTTAAAACTGAGACCCAGGGCA CCCGCAGACGACATGTTTGGAGTTGGGAATCAAAAACCTACTGCTGAGACAGCGAAAAGGAAAAACATCA AACGCAGACACACGCTTGGAGGCCACAGAGATGCTACTGAGATCAGCGTCCTGAGTTTCTGGAAAGCTCA TGAGCAGAGTGCGGACAAAGAGTCTGAACTTTCGGCTGTGAATCGGTTAAAACCCAAATGCTCAGCCCAG GACCTTTCCATCTCAGACTGGCTCGCCAGGGAGCGCGTCCGCACCAGTGCCTCCGACCTTAGCAGAGGCG AAGGCCTCGAGCCCCAGGCTGAGAGCCCCAGCGTGCTGGGAACACCCATCAGCACCCACTCACCACCGTC TCAGCAGCCCGAAGCAAGGGTTGCTGCTACCAGCACCTTGGCTTCAACCAGCCAGTCCCCTCTTTTCACA CCACCACAGTCACCTGACCAAATAAACAGAGAAAGCTTCCAGAACATGAGCCAAAATGCTAGTTCTACAG CTAACATCCACCCCCACAAACAGTCTGAAAGTCCAGACACTAAAGCAGAGACTCCTCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 76227. Forward Primer - name:076227_F_cDNA_Arhgap21, sequence:AAGAAGTCTGCTCGCTTCAAGT; Reverse Primer - name:076227_N_SP6_cDNA_Arhgap21, sequence:GGAGGAGTCTCTGCTTTAGTGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7770 same embryo
	EMAGE:7773 same embryo
	EMAGE:7774 same embryo
	EMAGE:7769 same embryo
	EMAGE:7771 same embryo
	EurExpress:euxassay_007662 same experiment
	MGI:4823218 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source