EMAGE mouse gene expression spatial database

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EMAGE:7776

Rnf220 ring finger protein 220 ( MGI:1913993)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007486. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007486_01	euxassay_007486_02	euxassay_007486_03	euxassay_007486_04

euxassay_007486_05	euxassay_007486_06	euxassay_007486_07	euxassay_007486_08	euxassay_007486_09

euxassay_007486_10	euxassay_007486_11	euxassay_007486_12	euxassay_007486_13	euxassay_007486_14

euxassay_007486_15	euxassay_007486_16	euxassay_007486_17	euxassay_007486_18	euxassay_007486_19

euxassay_007486_20	euxassay_007486_21	euxassay_007486_22	euxassay_007486_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7776_wholemount_strong.wlz

7776_wholemount_moderate.wlz

7776_wholemount_weak.wlz

7776_wholemount_possible.wlz

7776_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7776_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thalamus mantle layer

moderate

regional

moderate expression: see section 07 08 10 11 12 13 14 15 16 17 18

thalamus marginal layer

moderate

regional

moderate expression: see section 09

pons mantle layer

moderate

regional

moderate expression: see section 08 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9032
Entity Detected:	Rnf220, ring finger protein 220 ( MGI:1913993)
Sequence:	sense strand is shown >T9032 TGCTCTTCTCTCACACCAACAGAGTCTCTTGATCTGTACATGCAATCCCAGGCAGCTCGCGAGCACAAAC CCGGGGCCAGCCGCCTGCTGCTGCTGCTGCTGCCGCTGCCGCCACCACCGCCGCCGCTGCCCCCGCCGGC TCTGCGCACCCGGGACTTTTCATGCACCACACTCTCCGTCTTCTTACCTTGGTGTCCTGAAAAGTGCGAT CGTTCTCCCCAGGAATTTCCACGGTCTTCGAAGGGCATGATTCCCCAGTAATAATCCTCGCCCTGATCCA AGGTGCAGGTCGCCCTCCCTTCCAGGGAAGACTGCTTCTTGTGTGACACCGGCCATCGAAAGAGACTCCG ATGGACTTACACCGGGCAGCCTTCAAGATGGAGAACTCATCCTATCTTCCCAACCCTCTGGCGTCCCCAG CACTGATGGTCTTGGCGTCCACTGCCGAGGCCAGCAGGGATGCCTCCATCCCTTGTCAGCAGCCACGACC CTTTGGTGTACCTGTCTCAGTAGACAAGGACGTTCACATTCCCTTCACCAATGGCTCCTACACCTTTGCC TCTATGTACCACCGGCAAGGTGGGGTGCCGGGCACTTTTGCCAATCGTGATTTCCCCCCTTCCTTACTAC ACCTCCACCCCCAGTTTGCTCCCCCAAACCTAGATTGCACCCCAATCAGTATGCTAAATCACAGTGGTGT GGGGGCATTCCGTCCCTTCGCTTCCACGGAGGACAGGGAGAGCTATCAGTCTGCCTTTACTCCAGCCAAG AGACTCAAGAACTGCCATGACACAGAGTCTCCCCACCTGCGCTTCTCCGATGCAGATGGCAAGGAATATG ACTTTGGGACCCAGCTGCCATCCAGCTCCCCTGGCTCACTTAAGGTTGATGATACTGGGAAGAAGATCTT TGCTGTGTCTGGCCTCATCTCTGACCGGGAAACCTCCTCGAGTCCAGAGGATCGGAATGACAGATGTAAG AAGAAAGCAGTAGCGTTGTTTGACAGCCAAGCCCCGCTCTGCCCCATCTGCCAAGTCCTGCTGAGGCCCA GCGAGTTGCAGGAACATATGGAGCAGGAACTGGAACAGCTGGCCCAGCTGCCTGCCAGCAAAAATTCCCT TCTGAAGGATGCCATGGCTCCGGGCACCCCCAAGTCCCTCCTGTTGTCTGCTTCCATCAAGAGGGAAGGA GACTCTCCAACGGCTTCCCCACACTCTTCTGCCACGGAGGACCTGCACCACTCAGACAGATACCAGACCT TCCTGCGAGTGCGAGCCAACCGGCAAACCCGACTGAATGCTCGGATTGGGAAAATGAAACGGAGGAAGCA AGATGAAGGGCAGAGGGAAGGCTCCTGCATGGCTGAAGATGATGCTGTGGACATCGAGCATGCGGACAGC AACCGCTTTGAGGAATATGAGTGGTGTGGGCAGAAGCGGATACGGGCCACCACACTCCTGGAAGGTGGCT TCCGAGGCTCCGGCTTCGTCATGTGCAGTGGCAAAGAGAACCCAGACAGTGACGCTGACCTGGATGTGGA TGGAGATGACACACTGGAGTATGGGAAACCACAATACACGGAGGCTGATGTCATCCCGTGCACAGGAGAA GAGCCTGGTGAAGCCAAGGAGAGAGAGGCACTCCGGGGCGCAGTCCTAAATGGTGGCCCTCCGAGCACCC GCATCACACCTGAGTTCTCTAAATGGGCCAGTGATGAGATGCCATCCACCAGCAATGGTGAAGGCAGCAA GCAGGAGGCCATGCAGAAGACCTGCAAGAACAGCGACATTGAGAAAATCACCGAAGAGTCAGCTGTAACT ACATTTGAGGCTCTGAAGGCTCGGGTCCGGGAGCTTGAACGGCAGCTATCTCGGGGGGACCGATACAAAT GCCTCATCTGCATGGACTCTTACTCGATGCCTCTGACGTCGATCCAGTGTTGGCATGTGCACTGTGAAGA GTGTTGGCTGCGGACTCTGGGTGCCAAGAAGCTCTGCCCTCAGTGCAACACCATCACAGCCCCCGGAGAC CTGCGGAGGATCTACTTATGAGCTAGCTGTCTAGGCAGGCCTTGCCTCGAGCAACGCCCCTGCCCAGCCT CTGTGACAGTGACTCCCTCTCCCCTTGTACATACTCTCACACAGGTTCCCCTTGTACATACATGCACATA CACACCCAAACACACATAGAGGACTCTGGAGCCGCAGTGGGACAGCCCTGCCTGCTTGTTCTTCTGAACT TGGGGACCATGCCTCTTTGGGCTCTGCTTCCAGGATGGTTTGGGAAGCATAGCAGGGCACGGCTCGGCTC CTGAGATCCATCCCCTAGACTGACTGACCGACAGACACACAAACACCAGACTGAAGCACATGTAATATAG ACTGTGTATGTTTACAATGTTGTGTATAAATGGGACACTTTGCCCTCTACCACCTCCCCTTTGGTTGTAT GATTTTCTTCTTTTTTAATGACTCCTGGAAGCAGTGCCCCTTCAGAGCTGGCTGGGAGCTTGGCTCAGCC ACCTCTTAGGGTGCCTGCCTCTGCCATGGCATCCTGTCCCTTTCCCATGTGCTCGGTGTTCAGTGGTGTA TATTTCTTCTCCCAGACATGGGGCGTGTGCCCCAAGGGGTCTGATCCCCTCCTTAGTCTTAGCTCATGGG GCGGCTCATGAGGAGCTGGGGGCAGGGTAGAATGGGACCGAGCAGGAACAGAGGCTGCAGTTGGGCTGGC TGACTGAGGGTGCCTGGCTGCCCAGCCTGCGGTAGCCGTGCTCCCTGCCCTCCTGTGGCCCTATGGTGTG ATGCTGTGTCTGTATCTATGTGTCTATACAAAGGTACTTGCCCTTTCCCTTTGTAAACTACATTTGACAT GGATTAAAACAGTATAAACAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6813564 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6813564 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7778 same embryo
	EMAGE:7780 same embryo
	EMAGE:7775 same embryo
	EMAGE:7777 same embryo
	EMAGE:7779 same embryo
	EurExpress:euxassay_007486 same experiment
	MGI:4827775 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source