EMAGE mouse gene expression spatial database

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EMAGE:7807

Hars histidyl-tRNA synthetase ( MGI:108087)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005327. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005327_01	euxassay_005327_02	euxassay_005327_03	euxassay_005327_04

euxassay_005327_05	euxassay_005327_06	euxassay_005327_07	euxassay_005327_08	euxassay_005327_09

euxassay_005327_10	euxassay_005327_11	euxassay_005327_12	euxassay_005327_13	euxassay_005327_14

euxassay_005327_15	euxassay_005327_16	euxassay_005327_17	euxassay_005327_18	euxassay_005327_19

euxassay_005327_20	euxassay_005327_21	euxassay_005327_22	euxassay_005327_23	euxassay_005327_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7807_wholemount_strong.wlz

7807_wholemount_moderate.wlz

7807_wholemount_weak.wlz

7807_wholemount_possible.wlz

7807_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7807_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 09 10 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 08 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 17 18 19 moderate expression: see section 12 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7046
Entity Detected:	Hars, histidyl-tRNA synthetase ( MGI:108087)
Sequence:	sense strand is shown >T7046 GCGGGTTTTAGACAGCCGTCAGTTCCTGCTGGCAGGCAGGATGGCAGATCGTGCTGCTCTGGAGGAGCTG GTACGACTCCAGGGAGCGCATGTGAGGGGCCTTAAGGAGCAGAAAGCGTCCGCTGAGCAGATCGAGGAGG AGGTGACGAAACTCCTAAAACTGAAGGCACAGCTGGGCCAGGATGAAGGCAAACAGAAGTTTGTGCTCAA AACCCCCAAGGGCACGAGAGACTATAGTCCCCGGCAGATGGCAGTCCGGGAAAAGGTGTTTGATGTCATC ATCCGCTGTTTCAAACGCCATGGAGCAGAAGTGATTGATACACCTGTGTTTGAACTAAAGGAAACACTGA CTGGAAAGTACGGAGAAGACTCAAAGCTTATCTATGACTTGAAGGACCAGGGTGGGGAGCTGCTGTCCCT CCGTTATGACCTTACTGTCCCTTTTGCTCGCTATCTGGCAATGAATAAACTGACCAACATTAAACGCTAC CATATAGCAAAGGTGTATCGCCGGGACAACCCAGCTATGACCCGAGGCCGTTACCGTGAATTCTATCAAT GCGATTTTGACATTGCCGGACAGTTCGACCCCATGATTCCTGATGCAGAGTGCCTAAAGATTATGTGCGA GATCCTGAGTTCACTTCAGATCGGCAACTTCCTGGTCAAGGTAAACGACAGGCGCATCCTAGATGGGATG TTTGCTGTCTGTGGTGTTCCTGATAGCAAGTTCCGTACCATCTGCTCCTCGGTGGACAAGCTAGACAAGG TTTCCTGGGAGGAAGTGAAGAATGAGATGGTGGGAGAGAAGGGCCTTGCACCGGAAGTGGCTGATCGCAT CGGGGACTATGTCCAGCAGCATGGTGGGGTTTCTCTGGTGGAGCAGTTACTGCAGGATCCTAAACTCTCC CAAAACAAACAGGCTTTAGAGGGCTTGGGAGACCTAAAGCTGCTCTTTGAGTATCTAATCCTGTTTGGCA TTGATGACAAGATCTCCTTTGACCTGAGCCTTGCTCGGGGGCTAGACTACTATACTGGGGTCATCTATGA GGCAGTGCTGCTACAGATGCCAACCCAAGCTGGGGAAGAGCCCCTGGGTGTGGGCAGCATAGCTGCTGGA GGGCGCTACGATGGCCTGGTTGGCATGTTTGACCCCAAGGGGCGCAAGGTGCCATGCGTTGGGCTCAGTA TTGGAGTGGAGCGGATCTTTTCCATTGTAGAGCAAAGATTAGAGGCTTCAGAGGAAAAGGTACGGACCAC AGAGACACAGGTGCTTGTGGCATCTGCACAGAAGAAGCTGCTGGAGGAGAGACTGAAGCTTGTCTCAGAG TTATGGGATGCTGGGATCAAGGCTGAGCTGCTGTACAAGAAGAACCCGAAGTTACTAAACCAGTTGCAGT ACTGTGAGGAGGCAGGCATCCCACTGGTGGCCATCATTGGTGAGCAGGAACTCAAGGATGGCGTCATCAA GCTCCGCTCGGTGGCCAGCAGAGAGGAGGTGGATGTCCGAAGAGAAGACCTTGTGGAGGAAATCAGAAGA AGAACAAATCAGCCCCTCTCCATCTGCTGAGCTGAGCAAACCAGTAGAGGAAACAGGATTGATCTTAACT GAACGAAAACAAAACTGCATATGTACTTAAACAGTCTGCATATTTGCCATCCAGCAGAAGACTGAAGGGT GGTCAGAACAGAGGCTTTTTGTTTTGTTTTTGTTTTTATTATTATATTATCAGTAATCACTGGCAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4008479 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4008479 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7812 same embryo
	EMAGE:7810 same embryo
	EMAGE:7808 same embryo
	EMAGE:7809 same embryo
	EMAGE:7811 same embryo
	EurExpress:euxassay_005327 same experiment
	MGI:4825321 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source