EMAGE mouse gene expression spatial database

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EMAGE:7809

Smad4 MAD homolog 4 (Drosophila) ( MGI:894293)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005333. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005333_01	euxassay_005333_02	euxassay_005333_03	euxassay_005333_04

euxassay_005333_05	euxassay_005333_06	euxassay_005333_07	euxassay_005333_08	euxassay_005333_09

euxassay_005333_10	euxassay_005333_11	euxassay_005333_12	euxassay_005333_13	euxassay_005333_14

euxassay_005333_15	euxassay_005333_16	euxassay_005333_17	euxassay_005333_18	euxassay_005333_19

euxassay_005333_20	euxassay_005333_21	euxassay_005333_22	euxassay_005333_23	euxassay_005333_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7809_wholemount_strong.wlz

7809_wholemount_moderate.wlz

7809_wholemount_weak.wlz

7809_wholemount_possible.wlz

7809_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7809_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 09 10 16 17

pons mantle layer

moderate

regional

moderate expression: see section 08 18

facial vii ganglion

moderate

regional

moderate expression: see section 04 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 17

dorsal root ganglion

strong

regional

strong expression: see section 09 15 16 17 18 19 moderate expression: see section 07 08 10 11 12

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7774
Entity Detected:	Smad4, MAD homolog 4 (Drosophila) ( MGI:894293)
Sequence:	sense strand is shown >T7774 CCGCTGCGGTAACGGAGCGGCTCGGGTGGCGGAGCCCGTGTTCGCGTCCGTCCGCCCGCCCGCCCGCCGT CCTCCGGAGGCCCTTCCCGCGCCGCGCTCCGCTCCGCGGCCGTCCCCGGGGCGGGAGCGCGTGACCGGAG CCGGCGCCCGCGAGCGAGGCCCCCCGCAGCGGGGCGGCTCCGGAGCTCCAGCGGCCCGGCCGGCCGGCGC GGTCCGCGGCGCGGCGGGGAGAGGGGGCCGCCTGGGCCGGACGCCGCGGGCGGGGCCCGGGAAGCGACAG CGAGGCGAGGCGCGGTGCGGCGCGGAGCCCAGGTCATCCTGCTCACCAGATGTCTTGACAGTTTTTCTTG CAACATTGGCCATTGGTTTTCACTGCCTTCAAAAGATCAAAATTACTCCAGAAATTGGAGAGTTGGATTT AAAAGAAAAAACTTGAACAAATGGACAATATGTCTATAACAAATACACCAACAAGTAACGATGCCTGTCT GAGCATTGTACATAGTTTGATGTGTCATAGACAAGGTGGGGAAAGTGAAACCTTTGCAAAAAGAGCAATT GAGAGTTTGGTAAAGAAGCTGAAAGAGAAAAAAGATGAATTGGATTCTTTAATAACAGCTATAACTACAA ATGGAGCTCATCCTAGCAAGTGTGTCACCATACAGAGAACATTGGATGGACGACTTCAGGTGGCTGGTCG GAAAGGATTTCCTCATGTGATCTATGCCCGTCTGTGGAGGTGGCCTGATCTACACAAGAATGAACTAAAG CATGTTAAATATTGTCAGTATGCGTTTGACTTAAAATGTGACAGTGTCTGTGTGAATCCATATCACTATG AGCGGGTTGTCTCACCTGGAATTGATCTCTCAGGATTAACACTGCAGAGTAATGCTCCAAGTATGTTAGT GAAGGATGAGTACGTTCACGACTTTGAAGGACAGCCGTCCTTACCCACTGAAGGACATTCGATTCAAACC ATCCAACACCCGCCAAGTAATCGCGCATCAACGGAGACGTACAGCGCCCCGGCTCTGTTAGCCCCGGCAG AGTCTAACGCCACCAGCACCACCAACTTCCCCAACATTCCTGTGGCTTCCACAAGTCAGCCGGCCAGTAT TCTGGCGGGCAGCCATAGTGAAGGACTGTTGCAGATAGCTTCAGGGCCTCAGCCAGGACAGCAGCAGAAT GGATTTACTGCTCAGCCAGCTACTTACCATCATAACAGCACTACCACCTGGACTGGAAGTAGGACTGCAC CATACACACCTAATTTGCCTCACCACCAAAACGGCCATCTTCAGCACCACCCGCCTATGCCGCCCCATCC TGGACATTACTGGCCAGTTCACAATGAGCTTGCATTCCAGCCTCCCATTTCCAATCATCCTGCTCCTGAG TACTGGTGCTCCATTGCTTACTTTGAAATGGACGTTCAGGTAGGAGAGACGTTTAAGGTCCCTTCAAGCT GCCCTGTTGTGACTGTGGATGGCTATGTGGATCCTTCGGGAGGAGATCGCTTTTGCTTGGGTCAACTCTC CAATGTCCACAGGACAGAAGCGATTGAGAGAGCGAGGTTGCACATAGGCAAAGGAGTGCAGTTGGAATGT AAAGGTGAAGGTGACGTTTGGGTCAGGTGCCTTAGTGACCACGCGGTCTTTGTACAGAGTTACTACCTGG ACAGAGAAGCTGGCCGAGCACCTGGCGACGCTGTTCATAAGATCTACCCAAGCGCGTATATAAAGGTCTT TGATCTGCGGCAGTGTCACCGGCAGATGCAGCAACAGGCGGCCACTGCGCAAGCTGCAGCTGCTGCTCAG GCGGCGGCCGTGGCAGGGAACATCCCTGGCCCTGGGTCCGTGGGTGGAATAGCTCCAGCCATCAGTCTGT CTGCTGCTGCTGGCATCGGTGTGGATGACCTCCGGCGATTGTGCATTCTCAGGATGAGCTTTGTGAAGGG CTGGGGCCCAGACTACCCCAGGCAGAGCATCAAGGAAACCCCGTGCTGGATTGAGATTCACCTTCACCGA GCTCTGCAGCTCTTGGATGAAGTCCTGCACACCATGCCCATTGCGGACCCACAGCCTTTAGACTGAGATC TCACACCACGGACGCCCTAACCATTTCCAGGATGGTGGACTATGAAATATACTCGTGTTTATAATCTGAA GATCTATTGCATTTTGTTCTGCTCTGTCTTTTCCTAAAGGGTTGAGAGATGTGTTTGCTGCCTTGCTCTT AGCAGACAGAAACTGAATTAAAACTTCTTTTCTATTTTAGAACTTTCAGGTGTGGCTCAGTGCTTGAAGA TCAGAAAGATGCAGTTCTTGCTGAGTCTTCCCTGCTGGTTCTGTATGGAGGAGTCGGCCAGTGCTGGGCG CTCAGCCCTTTAGTGTGTGCGAGCGCCTTGCATGCCGAGGAGAGTCAGAGCTGCTGATTGTAAGGCTGAG AAGTTCTCACAGTTAAGCCACCTGCCCCTTAGTGGGCGAGTTATTAAACGCACTGTGCTCACGTGGCGCT GGGCCAGCCAGCTCTACCAAGAGCAACTTTACTCTCCTTTAAAAACCTTTTAGCAACCTTTGATTCACAA TGGTTTTTGCAAGTTAAACAGTGAAGGTGAATTAAATTCATACTGTCTTGCAGACTTCAGGGTTTCTTCC CCAAGACAAAACACTAATCTGTGTGCATATTGACAATTCCTTACAATTATCAGTCAAAGAAATGCCATTT AAAATTACAATTTTTTTAATCCCTAATGGATGACCACTATCAAGATGTATACTTTGCCCTGTTAAACAGT AAATGAATTCTTCTATATTTCTAGGCACAAGGTTAGTTATTTAAAAAAAAAAAAAAAAGCCTAGGGGAGG GATTTTTCCCTTAATTCCTAGGGAGAAGGTTTTGTATAAAACACTAAAAGCAGTGTCACTCTGCCTGCTG CTTCACTGTTCTGCAAGGTGGCAGTACTTCAACTGAAATAATGAATATTTTGGAAACTGCTAAATTCTAT GTTAAATACTGTGCAGAATAATGGAAACAGTGCAGTTGGTAACAGGTGGTTTGGATATTTTTGTACTTGA TTTGATGTGTGACTTCTTTTCATATACTGTTAAAATCATGTATGTTTTGACATTGTTTAAAATTCAGTTT TTGTATCTTAGGGCAAGACTGCAGACTTTTTTATACCTTTTGGTTATAAGCCCTGTGTTTGCCATCCTTG ATCACTTGGCGGTGACTTTGTAGAGATTGAAGTGGAGGAGTTAAGACACATTGACTGTACCACAGACACA CATGTATACTTTCTACCTAGTTACTAGCGTAAATAAAACTGAGTCACTATAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:6313280 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6313280 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7812 same embryo
	EMAGE:7807 same embryo
	EMAGE:7810 same embryo
	EMAGE:7808 same embryo
	EMAGE:7811 same embryo
	EurExpress:euxassay_005333 same experiment
	MGI:4828315 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source