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EMAGE:7890

Col25a1 collagen, type XXV, alpha 1 ( MGI:1924268)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7890 EMAGE:7890 EMAGE:7890 EMAGE:7890 EMAGE:7890
"Pseudo-wholemount" of euxassay_012820. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012820_01 euxassay_012820_02 euxassay_012820_03 euxassay_012820_04
EMAGE:7890 EMAGE:7890 EMAGE:7890 EMAGE:7890 EMAGE:7890
euxassay_012820_05 euxassay_012820_06 euxassay_012820_07 euxassay_012820_08 euxassay_012820_09
EMAGE:7890 EMAGE:7890 EMAGE:7890 EMAGE:7890 EMAGE:7890
euxassay_012820_10 euxassay_012820_11 euxassay_012820_12 euxassay_012820_13 euxassay_012820_14
EMAGE:7890 EMAGE:7890 EMAGE:7890 EMAGE:7890 EMAGE:7890
euxassay_012820_15 euxassay_012820_16 euxassay_012820_17 euxassay_012820_18 euxassay_012820_19
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euxassay_012820_20 euxassay_012820_21 euxassay_012820_22 euxassay_012820_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7890Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7890_wholemount_strong.wlz
7890_wholemount_moderate.wlz
7890_wholemount_weak.wlz
7890_wholemount_possible.wlz
7890_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7890_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38196
Entity Detected:Col25a1, collagen, type XXV, alpha 1 ( MGI:1924268)
Sequence:sense strand is shown

>T38196
GGTGATAAGGGAGAACAAGGTGATCAGGGACCTCGGATGGTGTTTCCTAAAATCAATCACGGCTTTCTCT
CTGCTGATCAGCAGCTCATTAAACGCCGGCTGATTAAGGGTGACCAAGGACAGGCAGGGCCTCCAGGACC
TCCAGGCCCTCCTGGTCCAAGAGGCCCACCTGGGGACACAGGAAAGGACGGCCCCCGAGGAATGCCAGGA
GTACCTGGTGAACCAGGAAAACCAGGAGAACAAGGCTTGATGGGACCTCTGGGGCCTCCAGGACAAAAGG
GTTCCATTGGAGCACCTGGGACCCCAGGCATGGATGGGCAAAAGGGTGAGCCTGGATCACCTGGAGCAGC
CGGGCAGAGTGGACTACCAGGACCTAAGGGAGAACCTGGAAAAGAAGGAGAAAAGGGAGATGCTGGAGAA
AATGGTCCCAAAGGTGATACAGGAGAAAAGGGTGACCCTGGATCATCTGCTGCAGGAATTAAGGGAGAAC
CTGGAGAATCTGGCCGCCCGGGGCAGAAGGGTGAACCAGGGCTGCCTGGGCTGCCTGGACTTCCGGGAAT
AAAGGGAGAACCAGGCTTCATTGGTCCTCAAGGAGAACCAGGGTTACCAGGGCTACCAGGAACAAAAGGT
GATCGTGGGGAGGCGGGGCCTCCTGGAAGAGGTGAACGAGGAGATCCTGGAGCCCCGGGGCCAAAGGGGA
AGCAAGGTGAATCAGGAGCTAGAGGCCCGAAGGGGTCAAAGGGTGATCGTGGAGACAAAGGAGACTCTGG
CGCTCTGGGACCACGGGGTCCACCTGGACAAAAGGGGGATCCAGGAGCCACAGAGATCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 104352. Forward Primer - name:104352_F_cDNA_Col25a1, sequence:GGTGATAAGGGAGAACAAGGTG; Reverse Primer - name:104352_N_SP6_cDNA_Col25a1, sequence:TGATCTCTGTGGCTCCTGGAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7888 same embryo
 EMAGE:7892 same embryo
 EMAGE:7891 same embryo
 EMAGE:7889 same embryo
 EurExpress:euxassay_012820 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS