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EMAGE:8194

Thap1 THAP domain containing, apoptosis associated protein 1 ( MGI:1921004)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8194 EMAGE:8194 EMAGE:8194 EMAGE:8194 EMAGE:8194
"Pseudo-wholemount" of euxassay_005334. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005334_01 euxassay_005334_02 euxassay_005334_03 euxassay_005334_04
EMAGE:8194 EMAGE:8194 EMAGE:8194 EMAGE:8194 EMAGE:8194
euxassay_005334_05 euxassay_005334_06 euxassay_005334_07 euxassay_005334_08 euxassay_005334_09
EMAGE:8194 EMAGE:8194 EMAGE:8194 EMAGE:8194 EMAGE:8194
euxassay_005334_10 euxassay_005334_11 euxassay_005334_12 euxassay_005334_13 euxassay_005334_14
EMAGE:8194 EMAGE:8194 EMAGE:8194 EMAGE:8194 EMAGE:8194
euxassay_005334_15 euxassay_005334_16 euxassay_005334_17 euxassay_005334_18 euxassay_005334_19
EMAGE:8194 EMAGE:8194
euxassay_005334_20 euxassay_005334_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8194Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8194_wholemount_strong.wlz
8194_wholemount_moderate.wlz
8194_wholemount_weak.wlz
8194_wholemount_possible.wlz
8194_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8194_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8172
Entity Detected:Thap1, THAP domain containing, apoptosis associated protein 1 ( MGI:1921004)
Sequence:sense strand is shown

>T8172
GCCATCTTGAAGATGGGAGACGGGCGACAGCTGTGGTCCTTCTGCTAAAGCAAACCCCACAACGGACAGG
GTAGTCACTCCCCCACCCCAACCCCCACCCCACGGCGAGGTGATCGTCCCCGTAACTGCTGACCGACGCC
ACCGAGAGCGGCGAGCGTTATCAAGGCCGAGCGCGGCGACCCCGACGGCCCCCTTCGCCTGCCTCCCGGG
CCGAAGGGAAGTGTGGAGGGCCAGAAGGATGGTGCAGTCCTGCTCCGCCTACGGCTGCAAGAACCGCTAC
GACAAGGACAAGCCCGTCTCCTTCCACAAGTTTCCTCTTACTCGCCCCAGCCTTTGTAAGCAGTGGGAGG
CAGCTGTTAAAAGGAAAAACTTCAAGCCCACCAAGTACAGCAGCATCTGCTCGGAGCACTTCACCCCGGA
CTGCTTTAAGAGGGAGTGCAACAACAAGCTACTGAAGGAGAACGCTGTGCCCACAATATTTCTCTATATC
GAGCCACATGAGAAGAAGGAAGACCTGGAATCCCAAGAACAGCTCCCCTCTCCTTCACCCCCCGCTTCCC
AGGTTGATGCTGCTATTGGGCTGCTAATGCCCCCTCTGCAGACCCCTGATAACCTGTCGGTTTTCTGTGA
CCACAATTACACTGTGGAGGATACGATGCACCAGAGGAAGAGGATCCTGCAGCTGGAGCAGCAGGTGGAG
AAACTCAGGAAGAAGCTCAAGACGGCCCAGCAGCGGTGCCGGCGGCAGGAGAGGCAGCTCGAGAAGCTCA
AGGAAGTCGTCCACTTTCAGAGAGAGAAGGACGACGCGTCCGAGAGGGGCTACGTGATCCTACCAAATGA
CTACTTTGAAATTGTTGAAGTTCCAGCATGAAAAAATGAGATGTGTTAGTGGGACAAGACTATACACCTT
CTTTTAGCCTACATACAGGAGTTCATTTGAAAAAATAACACTTAATTACTTGTATTAAAAAAACAATATT
TTTTTAAAATAAATTAGATATATACTGTAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5118383 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5118383 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8196 same embryo
 EMAGE:8195 same embryo
 EMAGE:8198 same embryo
 EMAGE:8197 same embryo
 EurExpress:euxassay_005334 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS