EMAGE mouse gene expression spatial database

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EMAGE:8197

Tcf15 transcription factor 15 ( MGI:104664)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007448. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007448_01	euxassay_007448_02	euxassay_007448_03	euxassay_007448_04

euxassay_007448_05	euxassay_007448_06	euxassay_007448_07	euxassay_007448_08	euxassay_007448_09

euxassay_007448_10	euxassay_007448_11	euxassay_007448_12	euxassay_007448_13	euxassay_007448_14

euxassay_007448_15	euxassay_007448_16	euxassay_007448_17	euxassay_007448_18	euxassay_007448_19

euxassay_007448_20	euxassay_007448_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8197_wholemount_strong.wlz

8197_wholemount_moderate.wlz

8197_wholemount_weak.wlz

8197_wholemount_possible.wlz

8197_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8197_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8175
Entity Detected:	Tcf15, transcription factor 15 ( MGI:104664)
Sequence:	sense strand is shown >T8175 CCCGCGGCCGGGCGCCCATGGCGTTCGCGCTGCTGCGCCCGGTCGGCGCGCACGTGCTGTACCCGGACGT GAGGCTGCTGAGCGAGGACGAGGAGAACCGCAGCGAGAGCGACGCGTCGGACCAGTCGTTCGGCTGCTGC GAAGGGCTGGAGGCGGCACGGCGTGGCCCGGGTCCCGGGAGCGGGCGGCGGGCGAGCAACGGCGCGGGCC CCGTGGTGGTGGTGCGGCAGCGACAGGCGGCCAACGCGCGCGAGCGAGACCGCACGCAGAGCGTGAACAC GGCCTTCACCGCCCTGCGCACGCTCATCCCCACCGAGCCCGTGGACCGAAAGCTGTCTAAGATCGAGACG CTGCGTCTGGCGTCCAGCTACATCGCCCACCTGGCCAACGTGCTGCTGCTGGGCGACGCGGCCGACGACG GGCAGCCGTGTTTCCGCGCGGCGGGCGGTGGCAAGAGCGCGGTCCCCGCCGCCGACGGCCGTCAGCCGCG CTCCATCTGCACCTTCTGTCTCAGCAACCAGCGCAAGGGGGGCAGCCGTCGTGACCTGGGGGGCAGCTGC TTGAAAGTGAGGGGTGTAGCCCCGCTCCGAGGGCCTCGGCGATGAACCTGGATCCCTGGTTTTCTCCAAG AAGGACTCCAGAGAAAGAGGCCATGAGCCAGCCCCTAGCTGGACAGAGGAGAAGATTCCAGAAGCCCAAA CCACTCCTGCGTTGTGTAAGGACCGGAGGACAATGGCCTGGGCACAGGGCTCACCCTGGAGAGCCACAAG GACCCATCTAGCCATCCAGGCCTAGCTGGCCAGGGACAAGGCAGAAATTTTAGAAAACCAAAGACTATTT TTGAAAGTGTGTGTGTGTGTCTGTGTGTCTGTGTGAATGTAGTATGTGAGCAAATCGGGGAGGGGGTCAA AATAAAGGTATTTTTAAATAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5143248 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5143248 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8196 same embryo
	EMAGE:8195 same embryo
	EMAGE:8194 same embryo
	EMAGE:8198 same embryo
	EurExpress:euxassay_007448 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source