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EMAGE:8397

Ptcd1 pentatricopeptide repeat domain 1 ( MGI:1919049)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8397 EMAGE:8397 EMAGE:8397 EMAGE:8397 EMAGE:8397
"Pseudo-wholemount" of euxassay_009535. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009535_01 euxassay_009535_02 euxassay_009535_03 euxassay_009535_04
EMAGE:8397 EMAGE:8397 EMAGE:8397 EMAGE:8397 EMAGE:8397
euxassay_009535_05 euxassay_009535_06 euxassay_009535_07 euxassay_009535_08 euxassay_009535_09
EMAGE:8397 EMAGE:8397 EMAGE:8397 EMAGE:8397 EMAGE:8397
euxassay_009535_10 euxassay_009535_11 euxassay_009535_12 euxassay_009535_13 euxassay_009535_14
EMAGE:8397 EMAGE:8397 EMAGE:8397 EMAGE:8397 EMAGE:8397
euxassay_009535_15 euxassay_009535_16 euxassay_009535_17 euxassay_009535_18 euxassay_009535_19
EMAGE:8397 EMAGE:8397 EMAGE:8397 EMAGE:8397 EMAGE:8397
euxassay_009535_20 euxassay_009535_21 euxassay_009535_22 euxassay_009535_23 euxassay_009535_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8397Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8397_wholemount_strong.wlz
8397_wholemount_moderate.wlz
8397_wholemount_weak.wlz
8397_wholemount_possible.wlz
8397_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8397_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36913
Entity Detected:Ptcd1, pentatricopeptide repeat domain 1 ( MGI:1919049)
Sequence:sense strand is shown

>T36913
GGAGCTGGAAGTCAGTCTCCTAAGTCTTGGAACTCTCTCCCCCGCTGTGGTCTCATTTGGGACAGTGGCC
ACACCTGCTGATAGGCTGGCGTTGATGGGGGGCCTGGAAGGCTTCCTGGGCAAGATGACCGAACACGGGC
TCCAGCCAGACATCAAAACCCTCACTCTGCTGGCTGAGGTGGTGGAGCCTGGGAGCGCTGCAGAATCCTC
GCTGCTTTCCGTCCTGGACAGGCATAGGGTGGAGGCCGACGTAACCTTCTTCAACACGCTGATAAGGAAA
AAGAGCAAACTGGGAGACCTGGAGGGAGCCAAGGCACTGTTGCCAATCCTGGCAAAGAAGGGAATTGTCC
CCAATCTTCGGACTTTCTGCAATCTGGCCATTGGTTGCCATCGGCCCAGGGATGGTATGCAGCTGCTTGC
AGACATGAAGAAGTCCCAGGTGAGCCCTAACATCCACATCTACAGCACCCTTATCAACGCAGCCCTCAAG
AAGTTGGACTACACCTATCTCATCAGCATCCTGAAGGACATGAGGCAGAACAGCGTCCCAGTAAACGAGG
TGGTCGTCCGCCAGCTGGAGTTTGCAGCCGAGTACCCTCCCACCTTTGACCGGTACAAAGGAAAAAACAC
CTACTTGGAGAAGATTGATGGCTTTCGGGCTTATTACAAGCAGTGGTTGAAAGCCATGCCAGCAGAGGAA
GCCCCCCACCCCTGGCAGGAGTTCCAGAACAAGCCTGTTGGGGACCAAGACACCACTGACAAGGCTGGCG
GCCTTAGGGACGGATGACGGAGCACGCGGCTATGACAAGTGCCCAGCCTTCACACTGCTGGGGGAGCCTG
GGAGTCCTTTCAACTTCAGAGGCCAAGAATATTCCAGAGGCACCACAGCACTTTACAGGCAAATGCTGGG
CTCAGGGTGCCCAGATCATAGCAGGTCCCACACCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 92404. Forward Primer - name:092404_F_cDNA_Ptcd1, sequence:GGAGCTGGAAGTCAGTCTCCTA; Reverse Primer - name:092404_N_SP6_cDNA_Ptcd1, sequence:TGGTGTGGGACCTGCTATGAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8395 same embryo
 EMAGE:8398 same embryo
 EMAGE:8394 same embryo
 EMAGE:8393 same embryo
 EMAGE:8396 same embryo
 EurExpress:euxassay_009535 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS