EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:8566

Ifngr2 interferon gamma receptor 2 ( MGI:107654)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009771. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009771_01	euxassay_009771_02	euxassay_009771_03	euxassay_009771_04

euxassay_009771_05	euxassay_009771_06	euxassay_009771_07	euxassay_009771_08	euxassay_009771_09

euxassay_009771_10	euxassay_009771_11	euxassay_009771_12	euxassay_009771_13	euxassay_009771_14

euxassay_009771_15	euxassay_009771_16	euxassay_009771_17	euxassay_009771_18	euxassay_009771_19

euxassay_009771_20	euxassay_009771_21	euxassay_009771_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8566_wholemount_strong.wlz

8566_wholemount_moderate.wlz

8566_wholemount_weak.wlz

8566_wholemount_possible.wlz

8566_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8566_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 07 08

cerebral cortex marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 07 08 14 15

pons mantle layer

strong

regional

strong expression: see section 06 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 16 17 weak expression: see section 02

vagus x ganglion

moderate

regional

moderate expression: see section 06 07 17

trigeminal v nerve

moderate

regional

moderate expression: see section 16

basal columns

moderate

regional

moderate expression: see section 07 08 09 10 11 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 13 14 15 weak expression: see section 08 11 12 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30632
Entity Detected:	Ifngr2, interferon gamma receptor 2 ( MGI:107654)
Sequence:	sense strand is shown >T30632 TCGCCAGACTCGTTTTCCCAGCTTGCGGCCCCTCTGAACCCAAGGCTTCACCTGTACAATGATGAGCAGA TTCTAACTTGGGAGCCGTCACCTTCCAGCAATGACCCAAGACCAGTGGTCTACCAGGTGGAATATAGCTT CATCGATGGCTCTTGGCATAGGTTGCTGGAGCCGAACTGTACGGACATCACAGAGACAAAGTGTGACTTA ACAGGAGGCGGCCGCTTGAAGCTTTTCCCACACCCATTCACAGTCTTCCTGCGGGTGCGAGCCAAGCGAG GGAACCTCACTTCCAAGTGGGTGGGGCTGGAGCCATTTCAACACTATGAGAATGTTACTGTTGGACCTCC GAAAAACATCTCGGTGACCCCAGGAAAAGGTTCCCTCGTCATACACTTCTCCCCTCCCTTTGATGTGTTC CACGGGGCAACTTTTCAGTATCTTGTCCACTACTGGGAAAAGTCAGAAACCCAACAGGAACAGGTTGAAG GCCCTTTCAAGAGCAACTCCATTGTGCTGGGCAATCTGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6403454), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60689. Forward Primer - name:060689_F_IRAV115_g07_Ifngr2, sequence:TCGCCAGACTCGTTTTCC; Reverse Primer - name:060689_R_SP6_IRAV115_g07_Ifngr2, sequence:TTCAGATTGCCCAGCACA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8570 same embryo
	EMAGE:8567 same embryo
	EMAGE:8565 same embryo
	EMAGE:8569 same embryo
	EMAGE:8568 same embryo
	EurExpress:euxassay_009771 same experiment
	MGI:4825526 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source