EMAGE mouse gene expression spatial database

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EMAGE:8569

Rnf38 ring finger protein 38 ( MGI:1920719)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009782. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009782_01	euxassay_009782_02	euxassay_009782_03	euxassay_009782_04

euxassay_009782_05	euxassay_009782_06	euxassay_009782_07	euxassay_009782_08	euxassay_009782_09

euxassay_009782_10	euxassay_009782_11	euxassay_009782_12	euxassay_009782_13	euxassay_009782_14

euxassay_009782_15	euxassay_009782_16	euxassay_009782_17	euxassay_009782_18	euxassay_009782_19

euxassay_009782_20	euxassay_009782_21	euxassay_009782_22	euxassay_009782_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8569_wholemount_strong.wlz

8569_wholemount_moderate.wlz

8569_wholemount_weak.wlz

8569_wholemount_possible.wlz

8569_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8569_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 06 18 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 07 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 16 17 18

spinal cord

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 11 12 13 14 15 16

neural retina

moderate

regional

moderate expression: see section 01 02 03 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 14 15 16 17 18

vomeronasal organ

moderate

regional

moderate expression: see section 12 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30631
Entity Detected:	Rnf38, ring finger protein 38 ( MGI:1920719)
Sequence:	sense strand is shown >T30631 TTTGGGTGTTCCTCATCACATGTATATATGGACTATCCATCGAACTTAACCTGTGTGGCTTCCAGCCCTC CCTTTACCAAAAGGGTCAATGGACCTTTCTTTGCACTGTGTGACTTAATCAACTATAAAAGCTTACAATT AGTCTTCACAATTATGGGATTGTTATACTAACTGTGTGATTGGAACTCCAAAGACCTTTTCTTTAGCTTA ATTTTGTGTGTGCACTAACATTCCCTGGTTTTTGTGTGATCATTCCGAGTGTTGCTGCAAGATTACAGTG GACGTGATCTTTTAGCATGTGCTTTTATAAAAAGTGGTAGCTCCAGATGATATAGCAATCTACCTTATAT AGAGCCTTCAGAAACTGTGAGTGGAAGTGAGTGAAGCGTATGACTGCTGGTGATCAGTGAATCTGTGAGA GAATGTGTGCAACTACATGTGTGGGGGCATGGTAGCTACTGTGTGTGTGAGATCCTATATACCAGCATGT ACCAAGAATGTGTGTGTAGTTTTAATTATGCTGCAATGTATAATCTGGTGTGTTATTTAAACAGCACTAG TACTGTACACTGGTTTTCCCTTGTGTTTGCTGTTGCACACTGATTGCTAAGGGTGCATCCATTCTAAGCA TTGAATACTCCATCCCCTTCCCGCCCAGTGAATGGAATGAGAAAAGCCTTCTTCCTTTGCTTCAGGCAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6408623), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59942. Forward Primer - name:059942_F_IRAV115_g04_Rnf38, sequence:TTTGGGTGTTCCTCATCACA; Reverse Primer - name:059942_R_SP6_IRAV115_g04_Rnf38, sequence:GCTGCCTGAAGCAAAGGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8570 same embryo
	EMAGE:8567 same embryo
	EMAGE:8566 same embryo
	EMAGE:8565 same embryo
	EMAGE:8568 same embryo
	EurExpress:euxassay_009782 same experiment
	MGI:4827778 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source